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DNA Sequence Variants in the Five Prime Untranslated Region of the Cyclooxygenase-2 Gene Are Commonly Found in Healthy Dogs and Gray Wolves.

Safra N, Hayward LJ, Aguilar M, Sacks BN, Westropp JL, Mohr FC, Mellersh CS, Bannasch DL - PLoS ONE (2015)

Bottom Line: Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy.We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs.Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.

ABSTRACT
The aim of this study was to investigate the frequency of regional DNA variants upstream to the translation initiation site of the canine Cyclooxygenase-2 (Cox-2) gene in healthy dogs. Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy. A role for Cox-2 DNA variants in genetic predisposition to canine renal dysplasia has been proposed and dog breeders have been encouraged to select against these DNA variants. We sequenced 272-422 bases in 152 dogs unaffected by renal dysplasia and found 19 different haplotypes including 11 genetic variants which had not been described previously. We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs. Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

No MeSH data available.


Related in: MedlinePlus

Alignment of DNA sequences upstream of the ATG of the Cox-2 gene from Human, Horse, Cow, Wolf, CanFam3.1 assembly, Mouse and Chicken.The presumed translation initiation ATG is boxed. A DNA variant (deletion of 12 nucleotides) is observed in CanFam3.1 sequence when compared to other organisms (double headed arrow).
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pone.0133127.g002: Alignment of DNA sequences upstream of the ATG of the Cox-2 gene from Human, Horse, Cow, Wolf, CanFam3.1 assembly, Mouse and Chicken.The presumed translation initiation ATG is boxed. A DNA variant (deletion of 12 nucleotides) is observed in CanFam3.1 sequence when compared to other organisms (double headed arrow).

Mentions: We next compared Cox-2 5’UTR sequences across species. mRNA sequences from Human (Homo sapiens; accession: U04636), Horse (Equus caballus; AF027334), Cow (Bos taurus; DT845401), Mouse (Mus musculus; BB653429), and Chicken (Gallus gallus; NM_001167719) were aligned together with genomic sequences from Wolf (Canis lupus; KP296709), and CanFam3.1 sequence (Canis lupus familiaris; canFam3_dna range = chr7:19674506–19674874). The alignment, shown in Fig 2, demonstrates that a conserved regional sequence includes ~12 additional nucleotides when compared to the CanFam3.1 assembly.


DNA Sequence Variants in the Five Prime Untranslated Region of the Cyclooxygenase-2 Gene Are Commonly Found in Healthy Dogs and Gray Wolves.

Safra N, Hayward LJ, Aguilar M, Sacks BN, Westropp JL, Mohr FC, Mellersh CS, Bannasch DL - PLoS ONE (2015)

Alignment of DNA sequences upstream of the ATG of the Cox-2 gene from Human, Horse, Cow, Wolf, CanFam3.1 assembly, Mouse and Chicken.The presumed translation initiation ATG is boxed. A DNA variant (deletion of 12 nucleotides) is observed in CanFam3.1 sequence when compared to other organisms (double headed arrow).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526539&req=5

pone.0133127.g002: Alignment of DNA sequences upstream of the ATG of the Cox-2 gene from Human, Horse, Cow, Wolf, CanFam3.1 assembly, Mouse and Chicken.The presumed translation initiation ATG is boxed. A DNA variant (deletion of 12 nucleotides) is observed in CanFam3.1 sequence when compared to other organisms (double headed arrow).
Mentions: We next compared Cox-2 5’UTR sequences across species. mRNA sequences from Human (Homo sapiens; accession: U04636), Horse (Equus caballus; AF027334), Cow (Bos taurus; DT845401), Mouse (Mus musculus; BB653429), and Chicken (Gallus gallus; NM_001167719) were aligned together with genomic sequences from Wolf (Canis lupus; KP296709), and CanFam3.1 sequence (Canis lupus familiaris; canFam3_dna range = chr7:19674506–19674874). The alignment, shown in Fig 2, demonstrates that a conserved regional sequence includes ~12 additional nucleotides when compared to the CanFam3.1 assembly.

Bottom Line: Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy.We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs.Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.

ABSTRACT
The aim of this study was to investigate the frequency of regional DNA variants upstream to the translation initiation site of the canine Cyclooxygenase-2 (Cox-2) gene in healthy dogs. Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy. A role for Cox-2 DNA variants in genetic predisposition to canine renal dysplasia has been proposed and dog breeders have been encouraged to select against these DNA variants. We sequenced 272-422 bases in 152 dogs unaffected by renal dysplasia and found 19 different haplotypes including 11 genetic variants which had not been described previously. We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs. Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

No MeSH data available.


Related in: MedlinePlus