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DNA Sequence Variants in the Five Prime Untranslated Region of the Cyclooxygenase-2 Gene Are Commonly Found in Healthy Dogs and Gray Wolves.

Safra N, Hayward LJ, Aguilar M, Sacks BN, Westropp JL, Mohr FC, Mellersh CS, Bannasch DL - PLoS ONE (2015)

Bottom Line: Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy.We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs.Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.

ABSTRACT
The aim of this study was to investigate the frequency of regional DNA variants upstream to the translation initiation site of the canine Cyclooxygenase-2 (Cox-2) gene in healthy dogs. Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy. A role for Cox-2 DNA variants in genetic predisposition to canine renal dysplasia has been proposed and dog breeders have been encouraged to select against these DNA variants. We sequenced 272-422 bases in 152 dogs unaffected by renal dysplasia and found 19 different haplotypes including 11 genetic variants which had not been described previously. We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs. Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

No MeSH data available.


Related in: MedlinePlus

DNA sequence variants identified within the 5’UTR of the Cox-2 gene in 152 dogs.Base positions upstream of the ATG translation initiation site (position zero) are noted on the left. *The exact base position for the duplicated sequence could not be determined. The sequence variant most commonly found in gray wolves is boxed.
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pone.0133127.g001: DNA sequence variants identified within the 5’UTR of the Cox-2 gene in 152 dogs.Base positions upstream of the ATG translation initiation site (position zero) are noted on the left. *The exact base position for the duplicated sequence could not be determined. The sequence variant most commonly found in gray wolves is boxed.

Mentions: The first coding ATG, according to dog mRNA sequence (GenBank: AY044905) represents position zero. The sequence variants identified upstream to position zero are described based on standard mutation nomenclature recommended by the Human Genome Variation Society (HGVS) [33], and in relation to CanFam3.1 sequence assembly. However, because of the repetitive high-GC content of the 5’UTR sequence of the Cox-2 gene, the exact base positioning of certain sequence variants could not be determined (Fig 1, S1 Table).


DNA Sequence Variants in the Five Prime Untranslated Region of the Cyclooxygenase-2 Gene Are Commonly Found in Healthy Dogs and Gray Wolves.

Safra N, Hayward LJ, Aguilar M, Sacks BN, Westropp JL, Mohr FC, Mellersh CS, Bannasch DL - PLoS ONE (2015)

DNA sequence variants identified within the 5’UTR of the Cox-2 gene in 152 dogs.Base positions upstream of the ATG translation initiation site (position zero) are noted on the left. *The exact base position for the duplicated sequence could not be determined. The sequence variant most commonly found in gray wolves is boxed.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526539&req=5

pone.0133127.g001: DNA sequence variants identified within the 5’UTR of the Cox-2 gene in 152 dogs.Base positions upstream of the ATG translation initiation site (position zero) are noted on the left. *The exact base position for the duplicated sequence could not be determined. The sequence variant most commonly found in gray wolves is boxed.
Mentions: The first coding ATG, according to dog mRNA sequence (GenBank: AY044905) represents position zero. The sequence variants identified upstream to position zero are described based on standard mutation nomenclature recommended by the Human Genome Variation Society (HGVS) [33], and in relation to CanFam3.1 sequence assembly. However, because of the repetitive high-GC content of the 5’UTR sequence of the Cox-2 gene, the exact base positioning of certain sequence variants could not be determined (Fig 1, S1 Table).

Bottom Line: Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy.We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs.Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.

ABSTRACT
The aim of this study was to investigate the frequency of regional DNA variants upstream to the translation initiation site of the canine Cyclooxygenase-2 (Cox-2) gene in healthy dogs. Cox-2 plays a role in various disease conditions such as acute and chronic inflammation, osteoarthritis and malignancy. A role for Cox-2 DNA variants in genetic predisposition to canine renal dysplasia has been proposed and dog breeders have been encouraged to select against these DNA variants. We sequenced 272-422 bases in 152 dogs unaffected by renal dysplasia and found 19 different haplotypes including 11 genetic variants which had not been described previously. We genotyped 7 gray wolves to ascertain the wildtype variant and found that the wolves we analyzed had predominantly the second most common DNA variant found in dogs. Our results demonstrate an elevated level of regional polymorphism that appears to be a feature of healthy domesticated dogs.

No MeSH data available.


Related in: MedlinePlus