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Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol).

Rocha Caldas GF, Oliveira AR, Araújo AV, Lafayette SS, Albuquerque GS, Silva-Neto Jda C, Costa-Silva JH, Ferreira F, Costa JG, Wanderley AG - PLoS ONE (2015)

Bottom Line: CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion.This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound.This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of the essential oil of Hyptis martiusii.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Universidade Federal de Pernambuco, 50740-521, Recife, PE, Brazil.

ABSTRACT
Recently, our research group identified and reported 1,8-cineole (CIN), a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM), as well as characterized the gastroprotective action of this oil. The aim of this study was to investigate the mechanisms of action involved in the antiulcer and healing activity of CIN, in order to confirm its correlation with the gastroprotective effect of EOHM. Wistar rats were exposed to different protocols (acute ulceration, gastrointestinal motility and antisecretory activity). In addition, were determinated the involvement of nitric oxide and sulphydryl groups; the levels of gastric mucus, lipid peroxidation, sulphydryl groups and myeloperoxidase activity. The healing ability was evaluated by acetic acid-induced chronic ulcer and histological and immunohistochemical analysis (PCNA, Ki-67 and BrdU). The treatment with CIN inhibited ethanol-, ethanol/HCl- and indomethacin-induced gastric lesions. The highest doses of CIN inhibited gastric emptying, but did not affect intestinal transit. CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion. CIN increased levels of mucus (89.3%), prevented depletion of -SH groups (62.6%) and reduced the level of lipid peroxidation (55.3%) and myeloperoxidase activity (59.4%) in the gastric mucosa. In chronic ulcer model, CIN reduced in 43.1% the gastric area lesion, promoted significant regeneration and restoration of the levels of mucus in glandular cells as confirmed by histological analysis; and promoted increase in cell proliferation as evidenced by reactivity for PCNA, Ki-67 and BrdU. This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound. This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of the essential oil of Hyptis martiusii.

No MeSH data available.


Related in: MedlinePlus

Effect of 1,8-cineole (CIN) on quantification of mucus (A), levels of sulfhydryl groups (B) levels of malondialdehyde (C) and myeloperoxidase activity (D) in the gastric ulcers model induced by ethanol.The non-injured control group (CN) received no treatment. The experimental groups received 1% Tween-80 aqueous solution (CL, injured control), pantoprazole (P, 40 mg/kg), N-acetylcysteine (NAC, 750 mg/kg) or CIN (100 mg/kg). Results are expressed as mean ± S. E. M for 6 animals. ANOVA followed by Tukey’s test (*p < 0.05 vs. non-injured control group-CN and #p < 0.05 vs. injured control group-CL).
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pone.0134558.g001: Effect of 1,8-cineole (CIN) on quantification of mucus (A), levels of sulfhydryl groups (B) levels of malondialdehyde (C) and myeloperoxidase activity (D) in the gastric ulcers model induced by ethanol.The non-injured control group (CN) received no treatment. The experimental groups received 1% Tween-80 aqueous solution (CL, injured control), pantoprazole (P, 40 mg/kg), N-acetylcysteine (NAC, 750 mg/kg) or CIN (100 mg/kg). Results are expressed as mean ± S. E. M for 6 animals. ANOVA followed by Tukey’s test (*p < 0.05 vs. non-injured control group-CN and #p < 0.05 vs. injured control group-CL).

Mentions: As shown in Fig 1A, the ethanol-injured control group animals showed a significant decrease in the levels of gastric mucus (2.8 ± 0.4 μg of Alcian Blue/g of tissue) compared to the non-injured control group (CN, 6.6 ± 0.4 μg of Alcian Blue/g of tissue). Treatment with CIN at a dose of 100 mg/kg was able to increase mucus production significantly in 89.3% (5.3 ± 0.5 μg of Alcian Blue/g of tissue) compared to the injured control group. Pantoprazole also caused a significant increase in the levels of gastric mucus (6.4 ± 0.5 μg of Alcian Blue/g of tissue).


Gastroprotective Mechanisms of the Monoterpene 1,8-Cineole (Eucalyptol).

Rocha Caldas GF, Oliveira AR, Araújo AV, Lafayette SS, Albuquerque GS, Silva-Neto Jda C, Costa-Silva JH, Ferreira F, Costa JG, Wanderley AG - PLoS ONE (2015)

Effect of 1,8-cineole (CIN) on quantification of mucus (A), levels of sulfhydryl groups (B) levels of malondialdehyde (C) and myeloperoxidase activity (D) in the gastric ulcers model induced by ethanol.The non-injured control group (CN) received no treatment. The experimental groups received 1% Tween-80 aqueous solution (CL, injured control), pantoprazole (P, 40 mg/kg), N-acetylcysteine (NAC, 750 mg/kg) or CIN (100 mg/kg). Results are expressed as mean ± S. E. M for 6 animals. ANOVA followed by Tukey’s test (*p < 0.05 vs. non-injured control group-CN and #p < 0.05 vs. injured control group-CL).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526535&req=5

pone.0134558.g001: Effect of 1,8-cineole (CIN) on quantification of mucus (A), levels of sulfhydryl groups (B) levels of malondialdehyde (C) and myeloperoxidase activity (D) in the gastric ulcers model induced by ethanol.The non-injured control group (CN) received no treatment. The experimental groups received 1% Tween-80 aqueous solution (CL, injured control), pantoprazole (P, 40 mg/kg), N-acetylcysteine (NAC, 750 mg/kg) or CIN (100 mg/kg). Results are expressed as mean ± S. E. M for 6 animals. ANOVA followed by Tukey’s test (*p < 0.05 vs. non-injured control group-CN and #p < 0.05 vs. injured control group-CL).
Mentions: As shown in Fig 1A, the ethanol-injured control group animals showed a significant decrease in the levels of gastric mucus (2.8 ± 0.4 μg of Alcian Blue/g of tissue) compared to the non-injured control group (CN, 6.6 ± 0.4 μg of Alcian Blue/g of tissue). Treatment with CIN at a dose of 100 mg/kg was able to increase mucus production significantly in 89.3% (5.3 ± 0.5 μg of Alcian Blue/g of tissue) compared to the injured control group. Pantoprazole also caused a significant increase in the levels of gastric mucus (6.4 ± 0.5 μg of Alcian Blue/g of tissue).

Bottom Line: CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion.This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound.This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of the essential oil of Hyptis martiusii.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Universidade Federal de Pernambuco, 50740-521, Recife, PE, Brazil.

ABSTRACT
Recently, our research group identified and reported 1,8-cineole (CIN), a monoterpene that naturally occur in many aromatic plants, as one of the major constituent of the essential oil from leaves of Hyptis martiusii (EOHM), as well as characterized the gastroprotective action of this oil. The aim of this study was to investigate the mechanisms of action involved in the antiulcer and healing activity of CIN, in order to confirm its correlation with the gastroprotective effect of EOHM. Wistar rats were exposed to different protocols (acute ulceration, gastrointestinal motility and antisecretory activity). In addition, were determinated the involvement of nitric oxide and sulphydryl groups; the levels of gastric mucus, lipid peroxidation, sulphydryl groups and myeloperoxidase activity. The healing ability was evaluated by acetic acid-induced chronic ulcer and histological and immunohistochemical analysis (PCNA, Ki-67 and BrdU). The treatment with CIN inhibited ethanol-, ethanol/HCl- and indomethacin-induced gastric lesions. The highest doses of CIN inhibited gastric emptying, but did not affect intestinal transit. CIN (100 mg/kg) reduced the volume of basal but not stimulated acid secretion. CIN increased levels of mucus (89.3%), prevented depletion of -SH groups (62.6%) and reduced the level of lipid peroxidation (55.3%) and myeloperoxidase activity (59.4%) in the gastric mucosa. In chronic ulcer model, CIN reduced in 43.1% the gastric area lesion, promoted significant regeneration and restoration of the levels of mucus in glandular cells as confirmed by histological analysis; and promoted increase in cell proliferation as evidenced by reactivity for PCNA, Ki-67 and BrdU. This findings demonstrate the role of 1,8-cineole as an important ulcer healing agent and indicate the involvement of antioxidant and cytoprotective mechanisms in the gastroprotective effect of compound. This study also provides evidence that 1,8-cineole is related to the gastroprotective effect of the essential oil of Hyptis martiusii.

No MeSH data available.


Related in: MedlinePlus