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Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity.

Di Luccia B, Crescenzo R, Mazzoli A, Cigliano L, Venditti P, Walser JC, Widmer A, Baccigalupi L, Ricca E, Iossa S - PLoS ONE (2015)

Bottom Line: Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups.The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples.The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University "Federico II" of Naples, Naples, Italy.

ABSTRACT
A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk of diabetes and cardiovascular diseases. Diet is also known to alter the microbial composition of the gut, although it is not clear whether such alteration contributes to the development of metabolic syndrome. The aim of this work was to assess the possible link between the gut microbiota and the development of diet-induced metabolic syndrome in a rat model of obesity. Rats were fed either a standard or high-fructose diet. Groups of fructose-fed rats were treated with either antibiotics or faecal samples from control rats by oral gavage. Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups. A 16S DNA-sequencing approach was used to evaluate the bacterial composition of the gut of animals under different diets. The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples. The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome. Our data indicate that in rats fed a fructose-rich diet the development of metabolic syndrome is directly correlated with variations of the gut content of specific bacterial taxa.

No MeSH data available.


Related in: MedlinePlus

Taxonomical units restored by faecal transplantation.The histograms show the restoration of two genera and one family distributions after faecal trasplantation. Their increase, due to the fructose-rich diet, is rescued by the faecal treatment. Values are reported as means±SEM of six different rats. * P< .05 (one-way ANOVA followed by Tukey post-test). C = control, F = fructose-fed, FT = fructose-fed+faecal samples.
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pone.0134893.g007: Taxonomical units restored by faecal transplantation.The histograms show the restoration of two genera and one family distributions after faecal trasplantation. Their increase, due to the fructose-rich diet, is rescued by the faecal treatment. Values are reported as means±SEM of six different rats. * P< .05 (one-way ANOVA followed by Tukey post-test). C = control, F = fructose-fed, FT = fructose-fed+faecal samples.

Mentions: For these eight genera and one family we then analyzed data of the FT group and observed that the representativeness of two genera, Coprococcus and Ruminococcus, and of the Coriobacteriaceae family was restored to levels similar to those observed in the C group (P < .05) (Fig 7). These results suggest a direct correlation between the number of members of the Coprococcus and Ruminococcus genera and of the Coriobacteriaceae family present in the gut and the development of the metabolic syndrome.


Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity.

Di Luccia B, Crescenzo R, Mazzoli A, Cigliano L, Venditti P, Walser JC, Widmer A, Baccigalupi L, Ricca E, Iossa S - PLoS ONE (2015)

Taxonomical units restored by faecal transplantation.The histograms show the restoration of two genera and one family distributions after faecal trasplantation. Their increase, due to the fructose-rich diet, is rescued by the faecal treatment. Values are reported as means±SEM of six different rats. * P< .05 (one-way ANOVA followed by Tukey post-test). C = control, F = fructose-fed, FT = fructose-fed+faecal samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526532&req=5

pone.0134893.g007: Taxonomical units restored by faecal transplantation.The histograms show the restoration of two genera and one family distributions after faecal trasplantation. Their increase, due to the fructose-rich diet, is rescued by the faecal treatment. Values are reported as means±SEM of six different rats. * P< .05 (one-way ANOVA followed by Tukey post-test). C = control, F = fructose-fed, FT = fructose-fed+faecal samples.
Mentions: For these eight genera and one family we then analyzed data of the FT group and observed that the representativeness of two genera, Coprococcus and Ruminococcus, and of the Coriobacteriaceae family was restored to levels similar to those observed in the C group (P < .05) (Fig 7). These results suggest a direct correlation between the number of members of the Coprococcus and Ruminococcus genera and of the Coriobacteriaceae family present in the gut and the development of the metabolic syndrome.

Bottom Line: Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups.The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples.The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University "Federico II" of Naples, Naples, Italy.

ABSTRACT
A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk of diabetes and cardiovascular diseases. Diet is also known to alter the microbial composition of the gut, although it is not clear whether such alteration contributes to the development of metabolic syndrome. The aim of this work was to assess the possible link between the gut microbiota and the development of diet-induced metabolic syndrome in a rat model of obesity. Rats were fed either a standard or high-fructose diet. Groups of fructose-fed rats were treated with either antibiotics or faecal samples from control rats by oral gavage. Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups. A 16S DNA-sequencing approach was used to evaluate the bacterial composition of the gut of animals under different diets. The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples. The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome. Our data indicate that in rats fed a fructose-rich diet the development of metabolic syndrome is directly correlated with variations of the gut content of specific bacterial taxa.

No MeSH data available.


Related in: MedlinePlus