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Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity.

Di Luccia B, Crescenzo R, Mazzoli A, Cigliano L, Venditti P, Walser JC, Widmer A, Baccigalupi L, Ricca E, Iossa S - PLoS ONE (2015)

Bottom Line: Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups.The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples.The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University "Federico II" of Naples, Naples, Italy.

ABSTRACT
A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk of diabetes and cardiovascular diseases. Diet is also known to alter the microbial composition of the gut, although it is not clear whether such alteration contributes to the development of metabolic syndrome. The aim of this work was to assess the possible link between the gut microbiota and the development of diet-induced metabolic syndrome in a rat model of obesity. Rats were fed either a standard or high-fructose diet. Groups of fructose-fed rats were treated with either antibiotics or faecal samples from control rats by oral gavage. Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups. A 16S DNA-sequencing approach was used to evaluate the bacterial composition of the gut of animals under different diets. The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples. The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome. Our data indicate that in rats fed a fructose-rich diet the development of metabolic syndrome is directly correlated with variations of the gut content of specific bacterial taxa.

No MeSH data available.


Related in: MedlinePlus

Relative Operational Taxonomic Units (OTUs) abundance at the Phylum (A) or Class (B) level in control (C), control+antibiotic (CA), fructose-fed (F), fructose-fed+antibiotic (FA) and fructose-fed+faecal samples (FT) rats.The bar plot shows how the caecal microbiota distribution of each sample changes in rats of the various groups.
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pone.0134893.g004: Relative Operational Taxonomic Units (OTUs) abundance at the Phylum (A) or Class (B) level in control (C), control+antibiotic (CA), fructose-fed (F), fructose-fed+antibiotic (FA) and fructose-fed+faecal samples (FT) rats.The bar plot shows how the caecal microbiota distribution of each sample changes in rats of the various groups.

Mentions: The identified phylotypes show that Firmicutes were the most abundant bacteria in all five diet groups (97–77%) while Bacteroidetes, Proteobacteria and Tenericutes were always less represented (19–2%, 2–0.5% and 0.2–0.07%, respectively) (Fig 4A). A significant increase in the number of Bacteroidetes was observed in the gut of animals treated with antibiotics (Fig 4A). At the class level, Clostridia are the most abundant organisms in all five groups, followed by Bacilli in groups C, F and FT and by Bacteroidia in CA and FA (Fig 4B). Independently from the diet regimen, Bacilli were almost totally absent in the gut of animals treated with antibiotics (Fig 4B).


Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity.

Di Luccia B, Crescenzo R, Mazzoli A, Cigliano L, Venditti P, Walser JC, Widmer A, Baccigalupi L, Ricca E, Iossa S - PLoS ONE (2015)

Relative Operational Taxonomic Units (OTUs) abundance at the Phylum (A) or Class (B) level in control (C), control+antibiotic (CA), fructose-fed (F), fructose-fed+antibiotic (FA) and fructose-fed+faecal samples (FT) rats.The bar plot shows how the caecal microbiota distribution of each sample changes in rats of the various groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526532&req=5

pone.0134893.g004: Relative Operational Taxonomic Units (OTUs) abundance at the Phylum (A) or Class (B) level in control (C), control+antibiotic (CA), fructose-fed (F), fructose-fed+antibiotic (FA) and fructose-fed+faecal samples (FT) rats.The bar plot shows how the caecal microbiota distribution of each sample changes in rats of the various groups.
Mentions: The identified phylotypes show that Firmicutes were the most abundant bacteria in all five diet groups (97–77%) while Bacteroidetes, Proteobacteria and Tenericutes were always less represented (19–2%, 2–0.5% and 0.2–0.07%, respectively) (Fig 4A). A significant increase in the number of Bacteroidetes was observed in the gut of animals treated with antibiotics (Fig 4A). At the class level, Clostridia are the most abundant organisms in all five groups, followed by Bacilli in groups C, F and FT and by Bacteroidia in CA and FA (Fig 4B). Independently from the diet regimen, Bacilli were almost totally absent in the gut of animals treated with antibiotics (Fig 4B).

Bottom Line: Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups.The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples.The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University "Federico II" of Naples, Naples, Italy.

ABSTRACT
A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk of diabetes and cardiovascular diseases. Diet is also known to alter the microbial composition of the gut, although it is not clear whether such alteration contributes to the development of metabolic syndrome. The aim of this work was to assess the possible link between the gut microbiota and the development of diet-induced metabolic syndrome in a rat model of obesity. Rats were fed either a standard or high-fructose diet. Groups of fructose-fed rats were treated with either antibiotics or faecal samples from control rats by oral gavage. Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups. A 16S DNA-sequencing approach was used to evaluate the bacterial composition of the gut of animals under different diets. The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples. The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome. Our data indicate that in rats fed a fructose-rich diet the development of metabolic syndrome is directly correlated with variations of the gut content of specific bacterial taxa.

No MeSH data available.


Related in: MedlinePlus