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Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods.

Xue C, Chen Y, Huang Y, Zhu P - Nanoscale Res Lett (2015)

Bottom Line: Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method.The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line.The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, 225009, China.

ABSTRACT
Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

No MeSH data available.


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Viability of MG-63 cells co-cultured with different nanorod concentrations
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Fig7: Viability of MG-63 cells co-cultured with different nanorod concentrations

Mentions: The in vitro biocompatibility of CHA nanorods was also assessed by MTT assay on MG-63 cell line. The MG-63 cells were co-cultured with CHA nanorods for 3 days at the concentration of 0, 20, 40, 60 μg/ml. As shown in Fig. 7, at the concentration of 20 μg/ml, the cell viability of CHA groups was higher than or equal to the HA group. However, at the concentration of 40 and 60 μg/ml, the cell viability of all CHA nanorods is a bit lower than the HA nanorod except CHA2 at a 40-μg/ml concentration, indicating that the carbonate contents have an impact on biocompatibility of nanorods. Moreover, even at the concentration of 60 μg/ml, all cell viability was still maintained above 75 %, proving that these CHA nanorods are biological apatites and biocompatible with human osteosarcoma MG-63 cell line.Fig. 7


Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods.

Xue C, Chen Y, Huang Y, Zhu P - Nanoscale Res Lett (2015)

Viability of MG-63 cells co-cultured with different nanorod concentrations
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526508&req=5

Fig7: Viability of MG-63 cells co-cultured with different nanorod concentrations
Mentions: The in vitro biocompatibility of CHA nanorods was also assessed by MTT assay on MG-63 cell line. The MG-63 cells were co-cultured with CHA nanorods for 3 days at the concentration of 0, 20, 40, 60 μg/ml. As shown in Fig. 7, at the concentration of 20 μg/ml, the cell viability of CHA groups was higher than or equal to the HA group. However, at the concentration of 40 and 60 μg/ml, the cell viability of all CHA nanorods is a bit lower than the HA nanorod except CHA2 at a 40-μg/ml concentration, indicating that the carbonate contents have an impact on biocompatibility of nanorods. Moreover, even at the concentration of 60 μg/ml, all cell viability was still maintained above 75 %, proving that these CHA nanorods are biological apatites and biocompatible with human osteosarcoma MG-63 cell line.Fig. 7

Bottom Line: Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method.The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line.The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, 225009, China.

ABSTRACT
Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

No MeSH data available.


Related in: MedlinePlus