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Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods.

Xue C, Chen Y, Huang Y, Zhu P - Nanoscale Res Lett (2015)

Bottom Line: Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method.The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line.The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, 225009, China.

ABSTRACT
Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

No MeSH data available.


Related in: MedlinePlus

ALP activity images of MG-63 cells co-cultured with nanorods: a HA; b CHA1; c CHA2; d CHA3
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Fig6: ALP activity images of MG-63 cells co-cultured with nanorods: a HA; b CHA1; c CHA2; d CHA3

Mentions: As shown in Fig. 6a–d, after co-culturing for 4 days with a 60-μg/ml concentration of nanorods, alkaline phosphatase (ALP) is expressed in large amounts in the cell cytosol of MG-63 cells. Alkaline phosphatase expression is indicative of osteogenesis [14]. The ALP activity results show that all synthesized CHA nanorods with different carbonate contents had similar impacts on the growth and osteogenic differentiation MG-63 cells.Fig. 6


Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods.

Xue C, Chen Y, Huang Y, Zhu P - Nanoscale Res Lett (2015)

ALP activity images of MG-63 cells co-cultured with nanorods: a HA; b CHA1; c CHA2; d CHA3
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526508&req=5

Fig6: ALP activity images of MG-63 cells co-cultured with nanorods: a HA; b CHA1; c CHA2; d CHA3
Mentions: As shown in Fig. 6a–d, after co-culturing for 4 days with a 60-μg/ml concentration of nanorods, alkaline phosphatase (ALP) is expressed in large amounts in the cell cytosol of MG-63 cells. Alkaline phosphatase expression is indicative of osteogenesis [14]. The ALP activity results show that all synthesized CHA nanorods with different carbonate contents had similar impacts on the growth and osteogenic differentiation MG-63 cells.Fig. 6

Bottom Line: Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method.The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line.The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, 225009, China.

ABSTRACT
Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

No MeSH data available.


Related in: MedlinePlus