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Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods.

Xue C, Chen Y, Huang Y, Zhu P - Nanoscale Res Lett (2015)

Bottom Line: Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method.The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line.The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, 225009, China.

ABSTRACT
Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

No MeSH data available.


Related in: MedlinePlus

Raman spectra of synthesized nanorods: a in the region 300–1200 cm-1, b in the region 3400–3700 cm-1
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Fig5: Raman spectra of synthesized nanorods: a in the region 300–1200 cm-1, b in the region 3400–3700 cm-1

Mentions: Raman spectra of all nanorods are shown in Fig. 5a, b. The characteristic peaks at 428 and 588 cm−1 were assigned to υ2 and υ4 mode, respectively [28]. As the carbonate content increases, the strongest symmetric stretch υ1 mode of PO43− at 960 cm−1 becomes broader, indicating the decrease of crystallinity of apatite lattices [1, 29]. The peak at 1070 cm−1 can be assigned to the B-type υ1 CO32− mode [30, 31]. Figure 5b shows the decrease in intensity of the O–H stretch at about 3571 cm−1 (normalized to the intensity of the 960 cm−1 peak) with increasing carbonate content. The O–H peak position slightly shifts upfield. The substitution of PO43− ions by CO32− ions may alter the chemical environment of OH ions to cause a shift in the vibrational frequency of O–H groups. As carbonate content increases, OH peak becomes broader due to the decreasing crystallinity, which is consistent with the broadening of the 960 cm−1 peak.Fig. 5


Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods.

Xue C, Chen Y, Huang Y, Zhu P - Nanoscale Res Lett (2015)

Raman spectra of synthesized nanorods: a in the region 300–1200 cm-1, b in the region 3400–3700 cm-1
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526508&req=5

Fig5: Raman spectra of synthesized nanorods: a in the region 300–1200 cm-1, b in the region 3400–3700 cm-1
Mentions: Raman spectra of all nanorods are shown in Fig. 5a, b. The characteristic peaks at 428 and 588 cm−1 were assigned to υ2 and υ4 mode, respectively [28]. As the carbonate content increases, the strongest symmetric stretch υ1 mode of PO43− at 960 cm−1 becomes broader, indicating the decrease of crystallinity of apatite lattices [1, 29]. The peak at 1070 cm−1 can be assigned to the B-type υ1 CO32− mode [30, 31]. Figure 5b shows the decrease in intensity of the O–H stretch at about 3571 cm−1 (normalized to the intensity of the 960 cm−1 peak) with increasing carbonate content. The O–H peak position slightly shifts upfield. The substitution of PO43− ions by CO32− ions may alter the chemical environment of OH ions to cause a shift in the vibrational frequency of O–H groups. As carbonate content increases, OH peak becomes broader due to the decreasing crystallinity, which is consistent with the broadening of the 960 cm−1 peak.Fig. 5

Bottom Line: Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method.The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line.The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, 225009, China.

ABSTRACT
Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.

No MeSH data available.


Related in: MedlinePlus