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Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.

Huang S, Liu X, Lao W, Zeng S, Liang H, Zhong R, Dai X, Wu X, Li H, Yao Y - BMC Infect. Dis. (2015)

Bottom Line: The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample.Drug resistance varied among different serotypes and age groups.Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, China. hsongyin@126.com.

ABSTRACT

Background: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.

Methods: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.

Results: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.

Conclusion: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

No MeSH data available.


Related in: MedlinePlus

Antibiotic susceptibility of S. pneumoniae isolates. a. Antibiotic susceptibility. b. Comparison of antibiotic resistance between patients ≤ 5 years and patients ≤ 51 years. c. Comparison of antibiotic resistance between serotype 19F and other serotypes. d. Comparison of antibiotic resistance between invasive and non-invasive S. pneumoniae isolates. * P < 0.05
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Fig3: Antibiotic susceptibility of S. pneumoniae isolates. a. Antibiotic susceptibility. b. Comparison of antibiotic resistance between patients ≤ 5 years and patients ≤ 51 years. c. Comparison of antibiotic resistance between serotype 19F and other serotypes. d. Comparison of antibiotic resistance between invasive and non-invasive S. pneumoniae isolates. * P < 0.05

Mentions: Antimicrobial susceptibilities determined for the clinical isolates analyzed in this study are shown in Fig. 3a and Additional file 1: Table S6. The percentage of these isolates that conferred resistance to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole was 91.2, 80.2, and 63.8 %, respectively; the percentage of isolates that conferred resistance to penicillin and amoxicillin was 47.3 and 34.1 %, respectively; and the percentage of isolates that conferred resistance to ceftriaxone and cefotaxime was 19.8 and 18.7 %, respectively. All isolates were susceptible to ertapenem, quinolone, linezolid and vancomycin, with the exception of one isolate (1.1 %) that was intermediately susceptible to ofloxacin, and 4 (4.4 %) isolates that were intermediately susceptible to ertapenem.Fig. 3


Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.

Huang S, Liu X, Lao W, Zeng S, Liang H, Zhong R, Dai X, Wu X, Li H, Yao Y - BMC Infect. Dis. (2015)

Antibiotic susceptibility of S. pneumoniae isolates. a. Antibiotic susceptibility. b. Comparison of antibiotic resistance between patients ≤ 5 years and patients ≤ 51 years. c. Comparison of antibiotic resistance between serotype 19F and other serotypes. d. Comparison of antibiotic resistance between invasive and non-invasive S. pneumoniae isolates. * P < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526307&req=5

Fig3: Antibiotic susceptibility of S. pneumoniae isolates. a. Antibiotic susceptibility. b. Comparison of antibiotic resistance between patients ≤ 5 years and patients ≤ 51 years. c. Comparison of antibiotic resistance between serotype 19F and other serotypes. d. Comparison of antibiotic resistance between invasive and non-invasive S. pneumoniae isolates. * P < 0.05
Mentions: Antimicrobial susceptibilities determined for the clinical isolates analyzed in this study are shown in Fig. 3a and Additional file 1: Table S6. The percentage of these isolates that conferred resistance to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole was 91.2, 80.2, and 63.8 %, respectively; the percentage of isolates that conferred resistance to penicillin and amoxicillin was 47.3 and 34.1 %, respectively; and the percentage of isolates that conferred resistance to ceftriaxone and cefotaxime was 19.8 and 18.7 %, respectively. All isolates were susceptible to ertapenem, quinolone, linezolid and vancomycin, with the exception of one isolate (1.1 %) that was intermediately susceptible to ofloxacin, and 4 (4.4 %) isolates that were intermediately susceptible to ertapenem.Fig. 3

Bottom Line: The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample.Drug resistance varied among different serotypes and age groups.Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, China. hsongyin@126.com.

ABSTRACT

Background: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.

Methods: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.

Results: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.

Conclusion: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

No MeSH data available.


Related in: MedlinePlus