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Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.

Huang S, Liu X, Lao W, Zeng S, Liang H, Zhong R, Dai X, Wu X, Li H, Yao Y - BMC Infect. Dis. (2015)

Bottom Line: The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample.Drug resistance varied among different serotypes and age groups.Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, China. hsongyin@126.com.

ABSTRACT

Background: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.

Methods: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.

Results: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.

Conclusion: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

No MeSH data available.


Related in: MedlinePlus

Serotype distribution and PCV immunization coverage among the S. pneumoniae isolates. a. Serotypes and immunization coverage rates of PCVs among all age groups. b. Serotypes and immunization coverage rates of PCVs among children ≤ 5 years. c. Serotypes and immunization coverage rates of PCVs among patients ≤ 51 years
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Fig2: Serotype distribution and PCV immunization coverage among the S. pneumoniae isolates. a. Serotypes and immunization coverage rates of PCVs among all age groups. b. Serotypes and immunization coverage rates of PCVs among children ≤ 5 years. c. Serotypes and immunization coverage rates of PCVs among patients ≤ 51 years

Mentions: Of the 94 S. pneumoniae isolates analyzed, 54 were identifiable by capsular serotyping. Of the remaining 40 isolates, 26 were identified using multiplex PCR, and 14 isolates (14.9 %) could not be serotyped. The serotype distribution of these S. pneumoniae isolates is shown in Fig. 2a and Additional file 1: Table S4. The most commonly isolated serotypes were 19F (n = 40, 42.6 %), 19A (n = 8, 8.5 %), 3 (n = 8, 8.5 %), 6B (n = 7, 7.4 %), and 23F (n = 4, 4.3 %). These five serotypes accounted for 71.3 % of the isolates. Serotypes 19F and 19A were the two dominant types. Interestingly, we did not find any recently discovered serotype 6C and 6D strains that were reportedly isolated from [18, 19].Fig. 2


Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.

Huang S, Liu X, Lao W, Zeng S, Liang H, Zhong R, Dai X, Wu X, Li H, Yao Y - BMC Infect. Dis. (2015)

Serotype distribution and PCV immunization coverage among the S. pneumoniae isolates. a. Serotypes and immunization coverage rates of PCVs among all age groups. b. Serotypes and immunization coverage rates of PCVs among children ≤ 5 years. c. Serotypes and immunization coverage rates of PCVs among patients ≤ 51 years
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526307&req=5

Fig2: Serotype distribution and PCV immunization coverage among the S. pneumoniae isolates. a. Serotypes and immunization coverage rates of PCVs among all age groups. b. Serotypes and immunization coverage rates of PCVs among children ≤ 5 years. c. Serotypes and immunization coverage rates of PCVs among patients ≤ 51 years
Mentions: Of the 94 S. pneumoniae isolates analyzed, 54 were identifiable by capsular serotyping. Of the remaining 40 isolates, 26 were identified using multiplex PCR, and 14 isolates (14.9 %) could not be serotyped. The serotype distribution of these S. pneumoniae isolates is shown in Fig. 2a and Additional file 1: Table S4. The most commonly isolated serotypes were 19F (n = 40, 42.6 %), 19A (n = 8, 8.5 %), 3 (n = 8, 8.5 %), 6B (n = 7, 7.4 %), and 23F (n = 4, 4.3 %). These five serotypes accounted for 71.3 % of the isolates. Serotypes 19F and 19A were the two dominant types. Interestingly, we did not find any recently discovered serotype 6C and 6D strains that were reportedly isolated from [18, 19].Fig. 2

Bottom Line: The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample.Drug resistance varied among different serotypes and age groups.Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, China. hsongyin@126.com.

ABSTRACT

Background: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.

Methods: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.

Results: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.

Conclusion: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

No MeSH data available.


Related in: MedlinePlus