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Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.

Huang S, Liu X, Lao W, Zeng S, Liang H, Zhong R, Dai X, Wu X, Li H, Yao Y - BMC Infect. Dis. (2015)

Bottom Line: The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample.Drug resistance varied among different serotypes and age groups.Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, China. hsongyin@126.com.

ABSTRACT

Background: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.

Methods: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.

Results: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.

Conclusion: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

No MeSH data available.


Related in: MedlinePlus

Serotype distribution among clinical specimens and age groups. a. Proportion and serotype distribution of S. pneumoniae isolates collected from different specimen sources. b. Proportion and serotype distribution of S. pneumoniae isolates among the different age groups
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Fig1: Serotype distribution among clinical specimens and age groups. a. Proportion and serotype distribution of S. pneumoniae isolates collected from different specimen sources. b. Proportion and serotype distribution of S. pneumoniae isolates among the different age groups

Mentions: A total of 94 S. pneumoniae isolates were prospectively collected from patients with pneumococcal infections at one Chinese hospital from 2011 to 2013. Of these, the most prevalent specimen sources were sputum and tracheal/bronchial aspirates (lower respiratory tract specimens) (n = 57, 60.6 %), followed by ear secretions (n = 14, 14.9 %), blood (n = 10, 10.6 %), CSF (n = 6, 6.4 %), and pleural fluid (n = 7, 7.4 %) (Fig. 1a, Additional file 1: Table S2). The rate of pneumococcal infections was higher in male patients (68 %) than in female patients (32 %), but this difference was not statistically significant (P = 0.61). Chaïbou et al. reported similar infection rates among males in Burkina Faso (257/476, 55 %) and findings by Bos et al. were similar among males in Mozambique (150/177, 85 %) [16, 17]. In contrast to patient sex, the serotype distribution displayed some age-dependent variation (Fig. 1b, Additional file 1: Table S3). Serotype proportions were significantly different between all three defined age groups (≤5 years, 6 ~ 50 years, and ≥ 51 years), but these differences may have been related to the limited number of serotypes present. Among the 94 isolates, 42 (44.7 %) were isolated from children (≤5 years) and 44 (46.8 %) were isolated from patients ≥ 51 years.Fig. 1


Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected at a Chinese hospital from 2011 to 2013.

Huang S, Liu X, Lao W, Zeng S, Liang H, Zhong R, Dai X, Wu X, Li H, Yao Y - BMC Infect. Dis. (2015)

Serotype distribution among clinical specimens and age groups. a. Proportion and serotype distribution of S. pneumoniae isolates collected from different specimen sources. b. Proportion and serotype distribution of S. pneumoniae isolates among the different age groups
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526307&req=5

Fig1: Serotype distribution among clinical specimens and age groups. a. Proportion and serotype distribution of S. pneumoniae isolates collected from different specimen sources. b. Proportion and serotype distribution of S. pneumoniae isolates among the different age groups
Mentions: A total of 94 S. pneumoniae isolates were prospectively collected from patients with pneumococcal infections at one Chinese hospital from 2011 to 2013. Of these, the most prevalent specimen sources were sputum and tracheal/bronchial aspirates (lower respiratory tract specimens) (n = 57, 60.6 %), followed by ear secretions (n = 14, 14.9 %), blood (n = 10, 10.6 %), CSF (n = 6, 6.4 %), and pleural fluid (n = 7, 7.4 %) (Fig. 1a, Additional file 1: Table S2). The rate of pneumococcal infections was higher in male patients (68 %) than in female patients (32 %), but this difference was not statistically significant (P = 0.61). Chaïbou et al. reported similar infection rates among males in Burkina Faso (257/476, 55 %) and findings by Bos et al. were similar among males in Mozambique (150/177, 85 %) [16, 17]. In contrast to patient sex, the serotype distribution displayed some age-dependent variation (Fig. 1b, Additional file 1: Table S3). Serotype proportions were significantly different between all three defined age groups (≤5 years, 6 ~ 50 years, and ≥ 51 years), but these differences may have been related to the limited number of serotypes present. Among the 94 isolates, 42 (44.7 %) were isolated from children (≤5 years) and 44 (46.8 %) were isolated from patients ≥ 51 years.Fig. 1

Bottom Line: The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample.Drug resistance varied among different serotypes and age groups.Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, China. hsongyin@126.com.

ABSTRACT

Background: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China.

Methods: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction.

Results: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older.

Conclusion: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.

No MeSH data available.


Related in: MedlinePlus