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Stimulation of osteoblast differentiation with guided ultrasound waves.

Macione J, Long D, Nesbitt S, Wentzell S, Yokota H, Pandit V, Kotha S - J Ther Ultrasound (2015)

Bottom Line: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells.In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034).The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180 USA.

ABSTRACT

Background: Ultrasound induces mechanical vibration and heat, causing differentiation and proliferation in osteoblasts. All known in vitro evaluations of ultrasound are, however, performed with longitudinal ultrasound waves. We addressed a question: Do other forms of ultrasound waves, such as guided waves (longitudinal and guided flexural) transduced at a remote location, enhance differentiation of osteoblast cells?

Methods: In this study, we employed guided Lamb waves that were induced in a borosilicate glass slide (cortical bone mimic). An average energy of 10-30 mW/cm(2) for 20 min per day was applied to MC3T3 osteoblast-like cells, which were placed 30-75 mm distant from the transducer.

Results: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells. In particular, guided waves elevated mRNA expression levels of bone formation-related genes such as alkaline phosphatase, osteopontin, osteocalcin, osteoprotegerin, and bone sialoprotein on days 8 and 16. In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034). The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

Conclusion: We demonstrate herein that ultrasound in a form of guided Lamb waves is capable of inducing osteoblast differentiation in vitro, and it may enable the stimulation of osteoblasts in vivo over a distance from the site of ultrasound application.

No MeSH data available.


Related in: MedlinePlus

a A schematic of a short section of a plate. b Exaggerated symmetric and antisymmetic flexural waves propagating through a plate at three closely related time points. c Both types of Lamb waves produce leaky waves which propagate out of the medium with respective directions. The symmetric half of the figure is from Lee and Kuo [6]
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Fig5: a A schematic of a short section of a plate. b Exaggerated symmetric and antisymmetic flexural waves propagating through a plate at three closely related time points. c Both types of Lamb waves produce leaky waves which propagate out of the medium with respective directions. The symmetric half of the figure is from Lee and Kuo [6]

Mentions: As the Lamb wave propagates, at a level of particle motion, there is flexion around its midline and allows energy to escape (Fig. 5). The flexion and extension create different types of Lamb waves which are classified as antisymmetric and symmetric modes, respectively. The wave motions of the antisymmetic and symmetric waves have different propagation velocities, a property that depends on d as well as the transducer frequency used to induce it.Fig. 5


Stimulation of osteoblast differentiation with guided ultrasound waves.

Macione J, Long D, Nesbitt S, Wentzell S, Yokota H, Pandit V, Kotha S - J Ther Ultrasound (2015)

a A schematic of a short section of a plate. b Exaggerated symmetric and antisymmetic flexural waves propagating through a plate at three closely related time points. c Both types of Lamb waves produce leaky waves which propagate out of the medium with respective directions. The symmetric half of the figure is from Lee and Kuo [6]
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526286&req=5

Fig5: a A schematic of a short section of a plate. b Exaggerated symmetric and antisymmetic flexural waves propagating through a plate at three closely related time points. c Both types of Lamb waves produce leaky waves which propagate out of the medium with respective directions. The symmetric half of the figure is from Lee and Kuo [6]
Mentions: As the Lamb wave propagates, at a level of particle motion, there is flexion around its midline and allows energy to escape (Fig. 5). The flexion and extension create different types of Lamb waves which are classified as antisymmetric and symmetric modes, respectively. The wave motions of the antisymmetic and symmetric waves have different propagation velocities, a property that depends on d as well as the transducer frequency used to induce it.Fig. 5

Bottom Line: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells.In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034).The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180 USA.

ABSTRACT

Background: Ultrasound induces mechanical vibration and heat, causing differentiation and proliferation in osteoblasts. All known in vitro evaluations of ultrasound are, however, performed with longitudinal ultrasound waves. We addressed a question: Do other forms of ultrasound waves, such as guided waves (longitudinal and guided flexural) transduced at a remote location, enhance differentiation of osteoblast cells?

Methods: In this study, we employed guided Lamb waves that were induced in a borosilicate glass slide (cortical bone mimic). An average energy of 10-30 mW/cm(2) for 20 min per day was applied to MC3T3 osteoblast-like cells, which were placed 30-75 mm distant from the transducer.

Results: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells. In particular, guided waves elevated mRNA expression levels of bone formation-related genes such as alkaline phosphatase, osteopontin, osteocalcin, osteoprotegerin, and bone sialoprotein on days 8 and 16. In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034). The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

Conclusion: We demonstrate herein that ultrasound in a form of guided Lamb waves is capable of inducing osteoblast differentiation in vitro, and it may enable the stimulation of osteoblasts in vivo over a distance from the site of ultrasound application.

No MeSH data available.


Related in: MedlinePlus