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Stimulation of osteoblast differentiation with guided ultrasound waves.

Macione J, Long D, Nesbitt S, Wentzell S, Yokota H, Pandit V, Kotha S - J Ther Ultrasound (2015)

Bottom Line: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells.In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034).The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180 USA.

ABSTRACT

Background: Ultrasound induces mechanical vibration and heat, causing differentiation and proliferation in osteoblasts. All known in vitro evaluations of ultrasound are, however, performed with longitudinal ultrasound waves. We addressed a question: Do other forms of ultrasound waves, such as guided waves (longitudinal and guided flexural) transduced at a remote location, enhance differentiation of osteoblast cells?

Methods: In this study, we employed guided Lamb waves that were induced in a borosilicate glass slide (cortical bone mimic). An average energy of 10-30 mW/cm(2) for 20 min per day was applied to MC3T3 osteoblast-like cells, which were placed 30-75 mm distant from the transducer.

Results: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells. In particular, guided waves elevated mRNA expression levels of bone formation-related genes such as alkaline phosphatase, osteopontin, osteocalcin, osteoprotegerin, and bone sialoprotein on days 8 and 16. In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034). The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

Conclusion: We demonstrate herein that ultrasound in a form of guided Lamb waves is capable of inducing osteoblast differentiation in vitro, and it may enable the stimulation of osteoblasts in vivo over a distance from the site of ultrasound application.

No MeSH data available.


Related in: MedlinePlus

a, b Representative images from alizarin red staining of both the control (a) and guided wave samples (b) at day 25. Quantifying the mineralizaiton (c) demonstrates that samples stimulated with guided waves have an average of 157 % more calcium, an effect which was independent of distance
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Fig4: a, b Representative images from alizarin red staining of both the control (a) and guided wave samples (b) at day 25. Quantifying the mineralizaiton (c) demonstrates that samples stimulated with guided waves have an average of 157 % more calcium, an effect which was independent of distance

Mentions: Alizarin red staining demonstrated that both the control and ultrasound-stimulated sample had nodule formation. However, the mineral deposition was increased in the samples stimulated by guided waves (Fig. 4a–c). Quantification of mineralization through a process of Alizarin red destaining revealed that an average of 157 % more mineralization was achieved in the stimulated samples (p = 0.034).Fig. 4


Stimulation of osteoblast differentiation with guided ultrasound waves.

Macione J, Long D, Nesbitt S, Wentzell S, Yokota H, Pandit V, Kotha S - J Ther Ultrasound (2015)

a, b Representative images from alizarin red staining of both the control (a) and guided wave samples (b) at day 25. Quantifying the mineralizaiton (c) demonstrates that samples stimulated with guided waves have an average of 157 % more calcium, an effect which was independent of distance
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526286&req=5

Fig4: a, b Representative images from alizarin red staining of both the control (a) and guided wave samples (b) at day 25. Quantifying the mineralizaiton (c) demonstrates that samples stimulated with guided waves have an average of 157 % more calcium, an effect which was independent of distance
Mentions: Alizarin red staining demonstrated that both the control and ultrasound-stimulated sample had nodule formation. However, the mineral deposition was increased in the samples stimulated by guided waves (Fig. 4a–c). Quantification of mineralization through a process of Alizarin red destaining revealed that an average of 157 % more mineralization was achieved in the stimulated samples (p = 0.034).Fig. 4

Bottom Line: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells.In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034).The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180 USA.

ABSTRACT

Background: Ultrasound induces mechanical vibration and heat, causing differentiation and proliferation in osteoblasts. All known in vitro evaluations of ultrasound are, however, performed with longitudinal ultrasound waves. We addressed a question: Do other forms of ultrasound waves, such as guided waves (longitudinal and guided flexural) transduced at a remote location, enhance differentiation of osteoblast cells?

Methods: In this study, we employed guided Lamb waves that were induced in a borosilicate glass slide (cortical bone mimic). An average energy of 10-30 mW/cm(2) for 20 min per day was applied to MC3T3 osteoblast-like cells, which were placed 30-75 mm distant from the transducer.

Results: The result revealed that guided waves significantly stimulated the differentiation and mineralization of MC3T3 cells. In particular, guided waves elevated mRNA expression levels of bone formation-related genes such as alkaline phosphatase, osteopontin, osteocalcin, osteoprotegerin, and bone sialoprotein on days 8 and 16. In addition, the amount of mineralization found via Alizarin red staining was increased by 157 % (p = 0.034). The amount of mineralization was found to be independent of distance from the transducer (p = 0.967).

Conclusion: We demonstrate herein that ultrasound in a form of guided Lamb waves is capable of inducing osteoblast differentiation in vitro, and it may enable the stimulation of osteoblasts in vivo over a distance from the site of ultrasound application.

No MeSH data available.


Related in: MedlinePlus