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The first morphometric and phylogenetic perspective on molecular epidemiology of Echinococcus granulosus sensu lato in stray dogs in a hyperendemic Middle East focus, northwestern Iran.

Shariatzadeh SA, Spotin A, Gholami S, Fallah E, Hazratian T, Mahami-Oskouei M, Montazeri F, Moslemzadeh HR, Shahbazi A - Parasit Vectors (2015)

Bottom Line: Six unique haplotypes were identified containing a high range of diversity (Haplotype diversity 0.873 vs.Nucleotide diversity 0.02).First presence of camel strain (G6) in this region seems to indicate that potential intermediate hosts play a secondary role in the maintenance of camel-dog biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. A.shariatzade@yahoo.com.

ABSTRACT

Background: Hydatidosis is considered to be a neglected cyclo-zoonotic disease in Middle East countries particularly northwestern Iran which is caused by metacestode of tapeworm Echinococcus granulosus sensu lato. Human hydatidosis is a high public health priority in the area, however there is little known from a morphometric and phylogenetic perspective on molecular epidemiology of adult Echinococcus spp. in Iranian stray dogs.

Methods: 80 dogs (38 males and 42 females) were collected during June 2013 to April 2014 in northwestern Iran. The isolated parasites from each dog were distinguished by morphometric keys including small, large hook length and blade length. Subsequently, isolates were confirmed by sequencing of mitochondrial cytochrome oxidase subunit 1 gene.

Results: 16 (8 males and 8 females) (Prevalence 20%) out of 80 dogs were infected to genus Echinococcus. With regard to demographic factors, the frequency of parasitism in both male, female adults and their age groups showed no difference (P > 0.05). The phylogenetic analyses of cox1 sequences firmly revealed the 13 sheep strains (G1), one buffalo strain (G3), one camel strain (G6) and one mixed infection. The findings of rostellar hook morphology show an intraspecies variation range among G1 isolates. However, hook measurements in Echinococcus derived from G1 (sheep strain) were not a significant difference from those G6 and G3 strains. Six unique haplotypes were identified containing a high range of diversity (Haplotype diversity 0.873 vs. Nucleotide diversity 0.02).

Conclusions: First presence of camel strain (G6) in this region seems to indicate that potential intermediate hosts play a secondary role in the maintenance of camel-dog biology. Current findings have heightened our knowledge about determination of Echinococcus prevalence, strains of taxonomy and genotypic trait of parasite in Iranian stray dogs which will also help in the development of strategies for monitoring and control of infected stray dogs in the area.

No MeSH data available.


Related in: MedlinePlus

Parsimonious haplotype network of mitochondrial DNA (Cox1) obtained from the 16 sequences. The size of the circles approximately indicates the number of individuals, and each mutation event is represented on the lines by a white circle. Haplotype AZE11 in G3 strain and Haplotype AZE10 in G6 strain are charecterized by green and red lines respectively. Haplotypes AZE01, 2, 4 and 5 in G1 strain have linked to common haplotye (AZE03) by black lines
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Fig2: Parsimonious haplotype network of mitochondrial DNA (Cox1) obtained from the 16 sequences. The size of the circles approximately indicates the number of individuals, and each mutation event is represented on the lines by a white circle. Haplotype AZE11 in G3 strain and Haplotype AZE10 in G6 strain are charecterized by green and red lines respectively. Haplotypes AZE01, 2, 4 and 5 in G1 strain have linked to common haplotye (AZE03) by black lines

Mentions: The nine common haplotypes AZE03 (Frequency: 56.25 %) were included without a notable heterogeneity in consensus position (GenBank Accession No; KP723338). The six unique haplotypes were included AZE11 (GenBank Accession No; KT154000) in G3 (Frequency: 6.26 %), AZE01 (GenBank Accession No; KT153999), AZE02 (GenBank Accession No; KT153998), AZE04 (GenBank Accession No; KT153997), AZE05 (GenBank Accession No; KT153996) in G1 (Frequency: 25 %) and AZE10 (GenBank Accession No; KT153995) in G6 (Frequency: 6.25 %). To discern a genealogical relationship among the haplotypes, we constructed a statistical parsimony network (Fig. 2). All GenBank accession numbers for the sequences inferred from this study and for the reference genotypes/species used in phylogenetic analysis are shown in Fig. 3.Fig. 2


The first morphometric and phylogenetic perspective on molecular epidemiology of Echinococcus granulosus sensu lato in stray dogs in a hyperendemic Middle East focus, northwestern Iran.

Shariatzadeh SA, Spotin A, Gholami S, Fallah E, Hazratian T, Mahami-Oskouei M, Montazeri F, Moslemzadeh HR, Shahbazi A - Parasit Vectors (2015)

Parsimonious haplotype network of mitochondrial DNA (Cox1) obtained from the 16 sequences. The size of the circles approximately indicates the number of individuals, and each mutation event is represented on the lines by a white circle. Haplotype AZE11 in G3 strain and Haplotype AZE10 in G6 strain are charecterized by green and red lines respectively. Haplotypes AZE01, 2, 4 and 5 in G1 strain have linked to common haplotye (AZE03) by black lines
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526285&req=5

Fig2: Parsimonious haplotype network of mitochondrial DNA (Cox1) obtained from the 16 sequences. The size of the circles approximately indicates the number of individuals, and each mutation event is represented on the lines by a white circle. Haplotype AZE11 in G3 strain and Haplotype AZE10 in G6 strain are charecterized by green and red lines respectively. Haplotypes AZE01, 2, 4 and 5 in G1 strain have linked to common haplotye (AZE03) by black lines
Mentions: The nine common haplotypes AZE03 (Frequency: 56.25 %) were included without a notable heterogeneity in consensus position (GenBank Accession No; KP723338). The six unique haplotypes were included AZE11 (GenBank Accession No; KT154000) in G3 (Frequency: 6.26 %), AZE01 (GenBank Accession No; KT153999), AZE02 (GenBank Accession No; KT153998), AZE04 (GenBank Accession No; KT153997), AZE05 (GenBank Accession No; KT153996) in G1 (Frequency: 25 %) and AZE10 (GenBank Accession No; KT153995) in G6 (Frequency: 6.25 %). To discern a genealogical relationship among the haplotypes, we constructed a statistical parsimony network (Fig. 2). All GenBank accession numbers for the sequences inferred from this study and for the reference genotypes/species used in phylogenetic analysis are shown in Fig. 3.Fig. 2

Bottom Line: Six unique haplotypes were identified containing a high range of diversity (Haplotype diversity 0.873 vs.Nucleotide diversity 0.02).First presence of camel strain (G6) in this region seems to indicate that potential intermediate hosts play a secondary role in the maintenance of camel-dog biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. A.shariatzade@yahoo.com.

ABSTRACT

Background: Hydatidosis is considered to be a neglected cyclo-zoonotic disease in Middle East countries particularly northwestern Iran which is caused by metacestode of tapeworm Echinococcus granulosus sensu lato. Human hydatidosis is a high public health priority in the area, however there is little known from a morphometric and phylogenetic perspective on molecular epidemiology of adult Echinococcus spp. in Iranian stray dogs.

Methods: 80 dogs (38 males and 42 females) were collected during June 2013 to April 2014 in northwestern Iran. The isolated parasites from each dog were distinguished by morphometric keys including small, large hook length and blade length. Subsequently, isolates were confirmed by sequencing of mitochondrial cytochrome oxidase subunit 1 gene.

Results: 16 (8 males and 8 females) (Prevalence 20%) out of 80 dogs were infected to genus Echinococcus. With regard to demographic factors, the frequency of parasitism in both male, female adults and their age groups showed no difference (P > 0.05). The phylogenetic analyses of cox1 sequences firmly revealed the 13 sheep strains (G1), one buffalo strain (G3), one camel strain (G6) and one mixed infection. The findings of rostellar hook morphology show an intraspecies variation range among G1 isolates. However, hook measurements in Echinococcus derived from G1 (sheep strain) were not a significant difference from those G6 and G3 strains. Six unique haplotypes were identified containing a high range of diversity (Haplotype diversity 0.873 vs. Nucleotide diversity 0.02).

Conclusions: First presence of camel strain (G6) in this region seems to indicate that potential intermediate hosts play a secondary role in the maintenance of camel-dog biology. Current findings have heightened our knowledge about determination of Echinococcus prevalence, strains of taxonomy and genotypic trait of parasite in Iranian stray dogs which will also help in the development of strategies for monitoring and control of infected stray dogs in the area.

No MeSH data available.


Related in: MedlinePlus