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CARD15 Gene Polymorphisms Are Associated with Tuberculosis Susceptibility in Chinese Holstein Cows.

Wang Y, Wang S, Liu T, Tu W, Li W, Dong G, Xu C, Qin B, Liu K, Yang J, Chai J, Shi X, Zhang Y - PLoS ONE (2015)

Bottom Line: SNPs in the CARD15 gene were assessed using polymerase chain reaction (PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR).The frequency of genotypes C/T, A/G, A/G, A/G, C/T, and A/G in E4 (-37), 208, 1644, 1648, 1799, and E10 (+107), respectively, was significantly higher in cases than in controls, and also the alleles C, A, A, G, T, and A, respectively, were associated with a greater relative risk in cases than in controls.The distribution of two haplotypes, TGGACA and CAGACA, was significantly different between cases and controls.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.

ABSTRACT
Bovine tuberculosis (BTB) is a significant veterinary and financial problem in many parts of the world. Associations between specific host genes and susceptibility to mycobacterial infections, such as tuberculosis, have been reported in several species. The objective of this study was to identify and evaluate the relationship of single-nucleotide polymorphisms (SNPs) in the CARD15 gene with susceptibility to BTB in Chinese Holstein cows. DNA samples from 201 Chinese Holstein cows (103 cases and 98 controls) were collected from Kunming City, Yuxi City, and Dali City in China. SNPs in the CARD15 gene were assessed using polymerase chain reaction (PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Case-control association testing and statistical analysis identified six SNPs associated with susceptibility to BTB in Chinese Holstein cows. The frequency of genotypes C/T, A/G, A/G, A/G, C/T, and A/G in E4 (-37), 208, 1644, 1648, 1799, and E10 (+107), respectively, was significantly higher in cases than in controls, and also the alleles C, A, A, G, T, and A, respectively, were associated with a greater relative risk in cases than in controls. The distribution of two haplotypes, TGGACA and CAGACA, was significantly different between cases and controls. Overall, this case-control study suggested that E4 (-37)(C/T), 208(A/G), 1644(A/G), 1648(A/G), 1799(C/T), and E10 (+107)(A/G) in the CARD15 gene were significantly associated with susceptibility to BTB in Chinese Holstein cows and that haplotypes TGGACA and CAGACA could be used as genetic markers in marker-assisted breeding programs for breeding cows with high resistance to BTB.

No MeSH data available.


Related in: MedlinePlus

Results of StyI-enzyme digestion at loci E10 (+109).M lane is the DL2000 marker, bands of 273, 253, 104, 20 bp for the A/G genotype in lane 1 and 20bp is invisible in the figure, bands of 253, 104, 20bp for G/G genotype in lanes 2,3, 4, 5, 7, bands of 273, 104 bp for A/A genotype in lane 6.
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pone.0135085.g001: Results of StyI-enzyme digestion at loci E10 (+109).M lane is the DL2000 marker, bands of 273, 253, 104, 20 bp for the A/G genotype in lane 1 and 20bp is invisible in the figure, bands of 253, 104, 20bp for G/G genotype in lanes 2,3, 4, 5, 7, bands of 273, 104 bp for A/A genotype in lane 6.

Mentions: The CARD15 gene in dairy cows includes 12 exons and 11 introns. It is located on Chromosome 18 and encodes 1013 amino acids. Of the 21 primers’ product that were designed in this study, 10 has polymorphisms and 13 SNPs were detected (Table 1). Three polymorphic loci could lead to an amino acid change; 208 (G/A) could change arginine (R) to histidine (H), 1644 (G/A) could change valine (V) to isoleucine (I), and 1648 (G/A) could change glycine (G) to glutamic acid (E). All other polymorphic loci [E2 (+718)(A/G), E2 (+1016)(A/G), E3 (+1303)(G/T), E4 (-37)(T/C), 1799(C/T), E5 (-68)(T/G), E7 (+349)(C/T), E9 (-257)(A/G), E10 (+107)(A/G), and 1531(A/C)] could not lead to an amino acid change. Of these 13 SNPs, E2 (+718)(A/G), E2 (+1016)(A/G), E3 (+1303)(G/T), E7 (+349)(C/T), and E10 (+107)(A/G) and 1531(A/C) were deposited in Genbank. Five SNPs, E4 (-37), 208, 1644, 1648, and 1799, that had not been studied previously and E10 (+107)(A/G)), which could be recognized by the restriction enzyme StyI, were selected and analyzed. Amplified fragment length of E10 (+107) was 377 bp and StyI enzyme recognized two digestion sites on this fragment (Fig 1). Fragment bands of 20 bp, 253 bp, and 104 bp were corresponded to genotype G/G; fragment bands of 273 bp and 104 bp were corresponded to genotype A/A; and fragment bands of 20 bp, 104 bp, 253 bp, and 273 bp were corresponded to genotype A/G The band of 20bp fragment was not always visible on the gel.


CARD15 Gene Polymorphisms Are Associated with Tuberculosis Susceptibility in Chinese Holstein Cows.

Wang Y, Wang S, Liu T, Tu W, Li W, Dong G, Xu C, Qin B, Liu K, Yang J, Chai J, Shi X, Zhang Y - PLoS ONE (2015)

Results of StyI-enzyme digestion at loci E10 (+109).M lane is the DL2000 marker, bands of 273, 253, 104, 20 bp for the A/G genotype in lane 1 and 20bp is invisible in the figure, bands of 253, 104, 20bp for G/G genotype in lanes 2,3, 4, 5, 7, bands of 273, 104 bp for A/A genotype in lane 6.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526225&req=5

pone.0135085.g001: Results of StyI-enzyme digestion at loci E10 (+109).M lane is the DL2000 marker, bands of 273, 253, 104, 20 bp for the A/G genotype in lane 1 and 20bp is invisible in the figure, bands of 253, 104, 20bp for G/G genotype in lanes 2,3, 4, 5, 7, bands of 273, 104 bp for A/A genotype in lane 6.
Mentions: The CARD15 gene in dairy cows includes 12 exons and 11 introns. It is located on Chromosome 18 and encodes 1013 amino acids. Of the 21 primers’ product that were designed in this study, 10 has polymorphisms and 13 SNPs were detected (Table 1). Three polymorphic loci could lead to an amino acid change; 208 (G/A) could change arginine (R) to histidine (H), 1644 (G/A) could change valine (V) to isoleucine (I), and 1648 (G/A) could change glycine (G) to glutamic acid (E). All other polymorphic loci [E2 (+718)(A/G), E2 (+1016)(A/G), E3 (+1303)(G/T), E4 (-37)(T/C), 1799(C/T), E5 (-68)(T/G), E7 (+349)(C/T), E9 (-257)(A/G), E10 (+107)(A/G), and 1531(A/C)] could not lead to an amino acid change. Of these 13 SNPs, E2 (+718)(A/G), E2 (+1016)(A/G), E3 (+1303)(G/T), E7 (+349)(C/T), and E10 (+107)(A/G) and 1531(A/C) were deposited in Genbank. Five SNPs, E4 (-37), 208, 1644, 1648, and 1799, that had not been studied previously and E10 (+107)(A/G)), which could be recognized by the restriction enzyme StyI, were selected and analyzed. Amplified fragment length of E10 (+107) was 377 bp and StyI enzyme recognized two digestion sites on this fragment (Fig 1). Fragment bands of 20 bp, 253 bp, and 104 bp were corresponded to genotype G/G; fragment bands of 273 bp and 104 bp were corresponded to genotype A/A; and fragment bands of 20 bp, 104 bp, 253 bp, and 273 bp were corresponded to genotype A/G The band of 20bp fragment was not always visible on the gel.

Bottom Line: SNPs in the CARD15 gene were assessed using polymerase chain reaction (PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR).The frequency of genotypes C/T, A/G, A/G, A/G, C/T, and A/G in E4 (-37), 208, 1644, 1648, 1799, and E10 (+107), respectively, was significantly higher in cases than in controls, and also the alleles C, A, A, G, T, and A, respectively, were associated with a greater relative risk in cases than in controls.The distribution of two haplotypes, TGGACA and CAGACA, was significantly different between cases and controls.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.

ABSTRACT
Bovine tuberculosis (BTB) is a significant veterinary and financial problem in many parts of the world. Associations between specific host genes and susceptibility to mycobacterial infections, such as tuberculosis, have been reported in several species. The objective of this study was to identify and evaluate the relationship of single-nucleotide polymorphisms (SNPs) in the CARD15 gene with susceptibility to BTB in Chinese Holstein cows. DNA samples from 201 Chinese Holstein cows (103 cases and 98 controls) were collected from Kunming City, Yuxi City, and Dali City in China. SNPs in the CARD15 gene were assessed using polymerase chain reaction (PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Case-control association testing and statistical analysis identified six SNPs associated with susceptibility to BTB in Chinese Holstein cows. The frequency of genotypes C/T, A/G, A/G, A/G, C/T, and A/G in E4 (-37), 208, 1644, 1648, 1799, and E10 (+107), respectively, was significantly higher in cases than in controls, and also the alleles C, A, A, G, T, and A, respectively, were associated with a greater relative risk in cases than in controls. The distribution of two haplotypes, TGGACA and CAGACA, was significantly different between cases and controls. Overall, this case-control study suggested that E4 (-37)(C/T), 208(A/G), 1644(A/G), 1648(A/G), 1799(C/T), and E10 (+107)(A/G) in the CARD15 gene were significantly associated with susceptibility to BTB in Chinese Holstein cows and that haplotypes TGGACA and CAGACA could be used as genetic markers in marker-assisted breeding programs for breeding cows with high resistance to BTB.

No MeSH data available.


Related in: MedlinePlus