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Effect of Prolonged Moderate Exercise on the Changes of Nonneuronal Cells in Early Myocardial Infarction.

Rinaldi B, Guida F, Furiano A, Donniacuo M, Luongo L, Gritti G, Urbanek K, Messina G, Maione S, Rossi F, de Novellis V - Neural Plast. (2015)

Bottom Line: In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach.We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation.Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Division of Pharmacology, The Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.

ABSTRACT
Myocardial infarction (MI) is one of the leading causes of death in developed countries and it is characterized by several associated symptomatologies and poor quality of life. Recent data showed a possible interaction between infarction and brain inflammation and activity. Previous studies have demonstrated the beneficial effect of exercise training on deterioration in cardiac function after MI. In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach. We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation. Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN. Our data suggest that there is an early brain reaction to myocardial infarction induction, involving nonneuronal cells, that is attenuated by the prolonged exercise.

No MeSH data available.


Related in: MedlinePlus

Effect of prolonged exercise on MI induced microglia and astrocyte morphological changes in the PVN. 48 h MI induced significant changes in the senescent (1.8 ± 0.1) (dystrophic-like) microglia in the PVN as compared to the sedentary group without MI (0.2 ± 0.1) (a, b). 48 h MI induced increased number of reactive astrocytes in the PVN (3.3 ± 0.51) versus control (1.5 ± 0.09). (c, d). Data are presented as mean ± SEM. ANOVA followed by Tukey post hoc test was used for statistical analysis. Four animals were used for each experimental group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus sedentary group. Scale bars 100 and 25 μm for panoramic and inset image, respectively.
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fig5: Effect of prolonged exercise on MI induced microglia and astrocyte morphological changes in the PVN. 48 h MI induced significant changes in the senescent (1.8 ± 0.1) (dystrophic-like) microglia in the PVN as compared to the sedentary group without MI (0.2 ± 0.1) (a, b). 48 h MI induced increased number of reactive astrocytes in the PVN (3.3 ± 0.51) versus control (1.5 ± 0.09). (c, d). Data are presented as mean ± SEM. ANOVA followed by Tukey post hoc test was used for statistical analysis. Four animals were used for each experimental group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus sedentary group. Scale bars 100 and 25 μm for panoramic and inset image, respectively.

Mentions: MI and sedentary rats did not differ in the number of total microglia as well as astrocytes in the PVN. Furthermore, MI surgical procedure did not change the number of activated microglia cells found in the control animals (~10%). However, MI rats showed an increased number of dystrophic microglia cells as well as hypertrophic astrocytes which were not affected by exercise training (Figures 5(a)–5(d)).


Effect of Prolonged Moderate Exercise on the Changes of Nonneuronal Cells in Early Myocardial Infarction.

Rinaldi B, Guida F, Furiano A, Donniacuo M, Luongo L, Gritti G, Urbanek K, Messina G, Maione S, Rossi F, de Novellis V - Neural Plast. (2015)

Effect of prolonged exercise on MI induced microglia and astrocyte morphological changes in the PVN. 48 h MI induced significant changes in the senescent (1.8 ± 0.1) (dystrophic-like) microglia in the PVN as compared to the sedentary group without MI (0.2 ± 0.1) (a, b). 48 h MI induced increased number of reactive astrocytes in the PVN (3.3 ± 0.51) versus control (1.5 ± 0.09). (c, d). Data are presented as mean ± SEM. ANOVA followed by Tukey post hoc test was used for statistical analysis. Four animals were used for each experimental group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus sedentary group. Scale bars 100 and 25 μm for panoramic and inset image, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4526216&req=5

fig5: Effect of prolonged exercise on MI induced microglia and astrocyte morphological changes in the PVN. 48 h MI induced significant changes in the senescent (1.8 ± 0.1) (dystrophic-like) microglia in the PVN as compared to the sedentary group without MI (0.2 ± 0.1) (a, b). 48 h MI induced increased number of reactive astrocytes in the PVN (3.3 ± 0.51) versus control (1.5 ± 0.09). (c, d). Data are presented as mean ± SEM. ANOVA followed by Tukey post hoc test was used for statistical analysis. Four animals were used for each experimental group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus sedentary group. Scale bars 100 and 25 μm for panoramic and inset image, respectively.
Mentions: MI and sedentary rats did not differ in the number of total microglia as well as astrocytes in the PVN. Furthermore, MI surgical procedure did not change the number of activated microglia cells found in the control animals (~10%). However, MI rats showed an increased number of dystrophic microglia cells as well as hypertrophic astrocytes which were not affected by exercise training (Figures 5(a)–5(d)).

Bottom Line: In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach.We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation.Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Division of Pharmacology, The Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.

ABSTRACT
Myocardial infarction (MI) is one of the leading causes of death in developed countries and it is characterized by several associated symptomatologies and poor quality of life. Recent data showed a possible interaction between infarction and brain inflammation and activity. Previous studies have demonstrated the beneficial effect of exercise training on deterioration in cardiac function after MI. In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach. We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation. Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN. Our data suggest that there is an early brain reaction to myocardial infarction induction, involving nonneuronal cells, that is attenuated by the prolonged exercise.

No MeSH data available.


Related in: MedlinePlus