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Effect of Prolonged Moderate Exercise on the Changes of Nonneuronal Cells in Early Myocardial Infarction.

Rinaldi B, Guida F, Furiano A, Donniacuo M, Luongo L, Gritti G, Urbanek K, Messina G, Maione S, Rossi F, de Novellis V - Neural Plast. (2015)

Bottom Line: In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach.We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation.Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Division of Pharmacology, The Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.

ABSTRACT
Myocardial infarction (MI) is one of the leading causes of death in developed countries and it is characterized by several associated symptomatologies and poor quality of life. Recent data showed a possible interaction between infarction and brain inflammation and activity. Previous studies have demonstrated the beneficial effect of exercise training on deterioration in cardiac function after MI. In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach. We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation. Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN. Our data suggest that there is an early brain reaction to myocardial infarction induction, involving nonneuronal cells, that is attenuated by the prolonged exercise.

No MeSH data available.


Related in: MedlinePlus

Infarct evaluation. Myocardial infarction 48 hours after coronary artery ligation. Hematoxylin and eosin staining with hypereosinophilic cardiomyocytes and early inflammatory infiltrates (upper panel). Confocal image of the infarcted heart (lower panel) showing apoptosis (TUNEL, green) and the disintegration of structural protein α-sarcomeric actin (red) in cardiomyocytes present in the infarcted area. The square represents a high magnification of the TUNEL positive profiles.
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fig1: Infarct evaluation. Myocardial infarction 48 hours after coronary artery ligation. Hematoxylin and eosin staining with hypereosinophilic cardiomyocytes and early inflammatory infiltrates (upper panel). Confocal image of the infarcted heart (lower panel) showing apoptosis (TUNEL, green) and the disintegration of structural protein α-sarcomeric actin (red) in cardiomyocytes present in the infarcted area. The square represents a high magnification of the TUNEL positive profiles.

Mentions: Forty-eight hours after coronary artery ligation, the presence of myocardial infarction was confirmed by histological analysis. Typical features of early myocardial infarction were present in H&E-stained sections. This was confirmed by the detection of apoptotic cardiomyocytes with a terminal deoxynucleotide transferase- (TdT-) mediated dUTP nick-end labeling (TUNEL) assay and confocal microscopy (Figure 1).


Effect of Prolonged Moderate Exercise on the Changes of Nonneuronal Cells in Early Myocardial Infarction.

Rinaldi B, Guida F, Furiano A, Donniacuo M, Luongo L, Gritti G, Urbanek K, Messina G, Maione S, Rossi F, de Novellis V - Neural Plast. (2015)

Infarct evaluation. Myocardial infarction 48 hours after coronary artery ligation. Hematoxylin and eosin staining with hypereosinophilic cardiomyocytes and early inflammatory infiltrates (upper panel). Confocal image of the infarcted heart (lower panel) showing apoptosis (TUNEL, green) and the disintegration of structural protein α-sarcomeric actin (red) in cardiomyocytes present in the infarcted area. The square represents a high magnification of the TUNEL positive profiles.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4526216&req=5

fig1: Infarct evaluation. Myocardial infarction 48 hours after coronary artery ligation. Hematoxylin and eosin staining with hypereosinophilic cardiomyocytes and early inflammatory infiltrates (upper panel). Confocal image of the infarcted heart (lower panel) showing apoptosis (TUNEL, green) and the disintegration of structural protein α-sarcomeric actin (red) in cardiomyocytes present in the infarcted area. The square represents a high magnification of the TUNEL positive profiles.
Mentions: Forty-eight hours after coronary artery ligation, the presence of myocardial infarction was confirmed by histological analysis. Typical features of early myocardial infarction were present in H&E-stained sections. This was confirmed by the detection of apoptotic cardiomyocytes with a terminal deoxynucleotide transferase- (TdT-) mediated dUTP nick-end labeling (TUNEL) assay and confocal microscopy (Figure 1).

Bottom Line: In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach.We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation.Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Division of Pharmacology, The Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.

ABSTRACT
Myocardial infarction (MI) is one of the leading causes of death in developed countries and it is characterized by several associated symptomatologies and poor quality of life. Recent data showed a possible interaction between infarction and brain inflammation and activity. Previous studies have demonstrated the beneficial effect of exercise training on deterioration in cardiac function after MI. In this study we analyzed in sedentary and trained rats the microglia and astrocytes 48 hours after MI in PVN, thalamus, prefrontal cortex, and hippocampus through immunofluorescence approach. We found significant changes in specific microglia phenotypes in the brain areas analyzed together with astrocytes activation. Prolonged exercise normalized these morphological changes of microglia and astrocytes in the prefrontal cortex, hippocampus, and thalamus but not in the PVN. Our data suggest that there is an early brain reaction to myocardial infarction induction, involving nonneuronal cells, that is attenuated by the prolonged exercise.

No MeSH data available.


Related in: MedlinePlus