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Plasmodium falciparum merozoite surface protein 1 block 2 gene polymorphism in field isolates along the slope of mount Cameroon: a cross - sectional study.

Apinjoh TO, Tata RB, Anchang-Kimbi JK, Chi HF, Fon EM, Mugri RN, Tangoh DA, Nyingchu RV, Ghogomu SM, Nkuo-Akenji T, Achidi EA - BMC Infect. Dis. (2015)

Bottom Line: All family specific allelic types (K1, MAD20 and RO33) as well as MR were observed in the different locations, with K1 being most abundant.Eighty seven (60 %) of individuals harbored more than one parasite clone, with a significant proportion (p = 0.009) in rural compared to other settings.These results indicate enormous diversity of P. falciparum in the area and suggests that allele specificity and complexity may be relevant for the progression to symptomatic disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon. apinjohtoby@yahoo.co.uk.

ABSTRACT

Background: Malaria remains a major global health burden despite the intensification of control efforts, due partly to the lack of an effective vaccine. Information on genetic diversity in natural parasite populations constitutes a major impediment to vaccine development efforts and is limited in some endemic settings. The present study characterized diversity by investigating msp1 block 2 polymorphisms and the relationship between the allele families with ethnodemographic indices and clinical phenotype.

Method: Individuals with asymptomatic parasitaemia (AP) or uncomplicated malaria (UM) were enrolled from rural, semi-rural and semi-urban localities at varying altitudes along the slope of mount Cameroon. P. falciparum malaria parasitaemic blood screened by light microscopy was depleted of leucocytes using CF11 cellulose columns and the parasite DNA genotyped by nested PCR.

Results: Length polymorphism was assessed in 151 field isolates revealing 64 (5) and 274 (22) distinct recombinant and major msp1 allelic fragments (genotypes) respectively. All family specific allelic types (K1, MAD20 and RO33) as well as MR were observed in the different locations, with K1 being most abundant. Eighty seven (60 %) of individuals harbored more than one parasite clone, with a significant proportion (p = 0.009) in rural compared to other settings. AP individuals had higher (p = 0.007) K1 allele frequencies but lower (p = 0.003) mean multiplicity of genotypes per infection (2.00 ± 0.98 vs. 2.56 ± 1.17) compared to UM patients.

Conclusions: These results indicate enormous diversity of P. falciparum in the area and suggests that allele specificity and complexity may be relevant for the progression to symptomatic disease.

No MeSH data available.


Related in: MedlinePlus

Fragment size polymorphisms (A1 and A2) and allele frequencies (B1 and B2) of msp1 block 2 allele families in participants with uncomplicated malaria (UM) and asymptomatic parasitaemia (AP) respectively
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Fig3: Fragment size polymorphisms (A1 and A2) and allele frequencies (B1 and B2) of msp1 block 2 allele families in participants with uncomplicated malaria (UM) and asymptomatic parasitaemia (AP) respectively

Mentions: The distribution of genotypes within the respective allelic families and their corresponding band size ranges in AP and UM individuals is presented on Fig. 3. The K1 allelic family was the most polymorphic, with thirteen distinct fragments, representing 10 genotypes while MAD20, MR and RO33 had 8, 5 and 4 different genotypes respectively. All msp1 family specific allelic types i.e. K1, MAD20 and RO33 as well as MR were observed in the different geographical locations (Table 4). In general, the K1 type was most abundant while RO33, the least abundant was detected in 46.2 % of the samples. Eighty seven (60 %) of individuals harbored more than one parasite clone, with some 39.3 % of the infections carrying two allelic types (K1/MAD20, K1/RO33, MAD20/RO33) whereas 30 samples contained all three major msp1 allelic types. The overall prevalence of the K1, MAD20 and MR alleles was similar across the different localities whereas the proportion of the RO33 allele (p = 0.007) as well as polyclonality (p = 0.009) was significantly different among the localities. In UM patients, all msp1 allele types were similar across localities while AP individuals in rural settings had the highest proportion of Ro33 (p = 0.003), MR (p = 0.034) and polyclonal infections (p = 0.023) (Table 4).Fig. 3


Plasmodium falciparum merozoite surface protein 1 block 2 gene polymorphism in field isolates along the slope of mount Cameroon: a cross - sectional study.

Apinjoh TO, Tata RB, Anchang-Kimbi JK, Chi HF, Fon EM, Mugri RN, Tangoh DA, Nyingchu RV, Ghogomu SM, Nkuo-Akenji T, Achidi EA - BMC Infect. Dis. (2015)

Fragment size polymorphisms (A1 and A2) and allele frequencies (B1 and B2) of msp1 block 2 allele families in participants with uncomplicated malaria (UM) and asymptomatic parasitaemia (AP) respectively
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526171&req=5

Fig3: Fragment size polymorphisms (A1 and A2) and allele frequencies (B1 and B2) of msp1 block 2 allele families in participants with uncomplicated malaria (UM) and asymptomatic parasitaemia (AP) respectively
Mentions: The distribution of genotypes within the respective allelic families and their corresponding band size ranges in AP and UM individuals is presented on Fig. 3. The K1 allelic family was the most polymorphic, with thirteen distinct fragments, representing 10 genotypes while MAD20, MR and RO33 had 8, 5 and 4 different genotypes respectively. All msp1 family specific allelic types i.e. K1, MAD20 and RO33 as well as MR were observed in the different geographical locations (Table 4). In general, the K1 type was most abundant while RO33, the least abundant was detected in 46.2 % of the samples. Eighty seven (60 %) of individuals harbored more than one parasite clone, with some 39.3 % of the infections carrying two allelic types (K1/MAD20, K1/RO33, MAD20/RO33) whereas 30 samples contained all three major msp1 allelic types. The overall prevalence of the K1, MAD20 and MR alleles was similar across the different localities whereas the proportion of the RO33 allele (p = 0.007) as well as polyclonality (p = 0.009) was significantly different among the localities. In UM patients, all msp1 allele types were similar across localities while AP individuals in rural settings had the highest proportion of Ro33 (p = 0.003), MR (p = 0.034) and polyclonal infections (p = 0.023) (Table 4).Fig. 3

Bottom Line: All family specific allelic types (K1, MAD20 and RO33) as well as MR were observed in the different locations, with K1 being most abundant.Eighty seven (60 %) of individuals harbored more than one parasite clone, with a significant proportion (p = 0.009) in rural compared to other settings.These results indicate enormous diversity of P. falciparum in the area and suggests that allele specificity and complexity may be relevant for the progression to symptomatic disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon. apinjohtoby@yahoo.co.uk.

ABSTRACT

Background: Malaria remains a major global health burden despite the intensification of control efforts, due partly to the lack of an effective vaccine. Information on genetic diversity in natural parasite populations constitutes a major impediment to vaccine development efforts and is limited in some endemic settings. The present study characterized diversity by investigating msp1 block 2 polymorphisms and the relationship between the allele families with ethnodemographic indices and clinical phenotype.

Method: Individuals with asymptomatic parasitaemia (AP) or uncomplicated malaria (UM) were enrolled from rural, semi-rural and semi-urban localities at varying altitudes along the slope of mount Cameroon. P. falciparum malaria parasitaemic blood screened by light microscopy was depleted of leucocytes using CF11 cellulose columns and the parasite DNA genotyped by nested PCR.

Results: Length polymorphism was assessed in 151 field isolates revealing 64 (5) and 274 (22) distinct recombinant and major msp1 allelic fragments (genotypes) respectively. All family specific allelic types (K1, MAD20 and RO33) as well as MR were observed in the different locations, with K1 being most abundant. Eighty seven (60 %) of individuals harbored more than one parasite clone, with a significant proportion (p = 0.009) in rural compared to other settings. AP individuals had higher (p = 0.007) K1 allele frequencies but lower (p = 0.003) mean multiplicity of genotypes per infection (2.00 ± 0.98 vs. 2.56 ± 1.17) compared to UM patients.

Conclusions: These results indicate enormous diversity of P. falciparum in the area and suggests that allele specificity and complexity may be relevant for the progression to symptomatic disease.

No MeSH data available.


Related in: MedlinePlus