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Protective Effects of Kaempferol against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β.

Zhou M, Ren H, Han J, Wang W, Zheng Q, Wang D - Oxid Med Cell Longev (2015)

Bottom Line: Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio.However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Shandong Provincial Qianfoshan Hospital, Jinan 250014, China ; Weifang Medical University, Weifang 261031, China.

ABSTRACT

Objective: This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats.

Method: Left ventricular developed pressure (LVDP) and its maximum up/down rate (±dp/dt max) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL). The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione/glutathione disulfide (GSH/GSSG) ratio, and tumor necrosis factor-alpha (TNF-α) were determined using enzyme linked immunosorbent assay (ELISA). Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.

Results: Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio. However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

Conclusion: These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3β activity in rats with I/R.

No MeSH data available.


Related in: MedlinePlus

Kaempferol reduces inflammatory response. Values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.
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fig3: Kaempferol reduces inflammatory response. Values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.

Mentions: As shown in Figure 3, the level of TNF-α increased from 95 ± 4.5 pg/mL in the control group to 328 ± 16.7 pg/mL in the I/R group. The levels of TNF-α in the kaempferol (168 ± 9.3 pg/mL) and TDZD-8 groups (197 ± 11.4 pg/mL) significantly decreased compared with that in the I/R group (P < 0.01).


Protective Effects of Kaempferol against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β.

Zhou M, Ren H, Han J, Wang W, Zheng Q, Wang D - Oxid Med Cell Longev (2015)

Kaempferol reduces inflammatory response. Values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4525766&req=5

fig3: Kaempferol reduces inflammatory response. Values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.
Mentions: As shown in Figure 3, the level of TNF-α increased from 95 ± 4.5 pg/mL in the control group to 328 ± 16.7 pg/mL in the I/R group. The levels of TNF-α in the kaempferol (168 ± 9.3 pg/mL) and TDZD-8 groups (197 ± 11.4 pg/mL) significantly decreased compared with that in the I/R group (P < 0.01).

Bottom Line: Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio.However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Shandong Provincial Qianfoshan Hospital, Jinan 250014, China ; Weifang Medical University, Weifang 261031, China.

ABSTRACT

Objective: This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats.

Method: Left ventricular developed pressure (LVDP) and its maximum up/down rate (±dp/dt max) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL). The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione/glutathione disulfide (GSH/GSSG) ratio, and tumor necrosis factor-alpha (TNF-α) were determined using enzyme linked immunosorbent assay (ELISA). Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.

Results: Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio. However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

Conclusion: These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3β activity in rats with I/R.

No MeSH data available.


Related in: MedlinePlus