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Protective Effects of Kaempferol against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β.

Zhou M, Ren H, Han J, Wang W, Zheng Q, Wang D - Oxid Med Cell Longev (2015)

Bottom Line: Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio.However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Shandong Provincial Qianfoshan Hospital, Jinan 250014, China ; Weifang Medical University, Weifang 261031, China.

ABSTRACT

Objective: This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats.

Method: Left ventricular developed pressure (LVDP) and its maximum up/down rate (±dp/dt max) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL). The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione/glutathione disulfide (GSH/GSSG) ratio, and tumor necrosis factor-alpha (TNF-α) were determined using enzyme linked immunosorbent assay (ELISA). Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.

Results: Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio. However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

Conclusion: These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3β activity in rats with I/R.

No MeSH data available.


Related in: MedlinePlus

Kaempferol reduces the I/R-induced IS. (a) Images of myocardial tissue sections after TTC staining. (b) The ratio between IS and AAR, in which AAR is the area at risk and IS is the infarct size; values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.
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fig1: Kaempferol reduces the I/R-induced IS. (a) Images of myocardial tissue sections after TTC staining. (b) The ratio between IS and AAR, in which AAR is the area at risk and IS is the infarct size; values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.

Mentions: The representative slices of the hearts are shown in Figure 1(a). The ratio of IS and AAR in the control group was 5.31% ± 1.34%, which was significantly different from that of the I/R group (46.73% ± 1.88%) (P < 0.01; Figure 1(b)). The ratio remarkably decreased in the kaempferol (16.49% ± 1.23%) and TDZD-8 groups (21.42% ± 1.48%) compared with that in the I/R group (P < 0.01; Figure 1(b)).


Protective Effects of Kaempferol against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β.

Zhou M, Ren H, Han J, Wang W, Zheng Q, Wang D - Oxid Med Cell Longev (2015)

Kaempferol reduces the I/R-induced IS. (a) Images of myocardial tissue sections after TTC staining. (b) The ratio between IS and AAR, in which AAR is the area at risk and IS is the infarct size; values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4525766&req=5

fig1: Kaempferol reduces the I/R-induced IS. (a) Images of myocardial tissue sections after TTC staining. (b) The ratio between IS and AAR, in which AAR is the area at risk and IS is the infarct size; values are presented as means with their standard deviations (, n = 8); ∗∗P < 0.01, compared with the control group; ##P < 0.01, compared with the I/R group.
Mentions: The representative slices of the hearts are shown in Figure 1(a). The ratio of IS and AAR in the control group was 5.31% ± 1.34%, which was significantly different from that of the I/R group (46.73% ± 1.88%) (P < 0.01; Figure 1(b)). The ratio remarkably decreased in the kaempferol (16.49% ± 1.23%) and TDZD-8 groups (21.42% ± 1.48%) compared with that in the I/R group (P < 0.01; Figure 1(b)).

Bottom Line: Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio.However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Shandong Provincial Qianfoshan Hospital, Jinan 250014, China ; Weifang Medical University, Weifang 261031, China.

ABSTRACT

Objective: This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats.

Method: Left ventricular developed pressure (LVDP) and its maximum up/down rate (±dp/dt max) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL). The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione/glutathione disulfide (GSH/GSSG) ratio, and tumor necrosis factor-alpha (TNF-α) were determined using enzyme linked immunosorbent assay (ELISA). Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.

Results: Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dt max, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio. However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.

Conclusion: These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3β activity in rats with I/R.

No MeSH data available.


Related in: MedlinePlus