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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8.

Hong Z, Hong H, Liu J, Zheng X, Huang M, Li C, Xia J - Mediators Inflamm. (2015)

Bottom Line: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China.

ABSTRACT

Aims: This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.

Materials and methods: miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3'UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.

Results: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.

Conclusions: This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

No MeSH data available.


Related in: MedlinePlus

Serum IL-8 protein and mRNA levels were significantly increased in CHB patients and were positively correlated with serum ALT. (a) Serum IL-8 and PBMCs IL-8 mRNA of 56 CHB patients and 40 healthy controls were assessed by ELISA and qRT-PCR. (b) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with serum ALT levels. (c) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with TBIL levels.
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fig2: Serum IL-8 protein and mRNA levels were significantly increased in CHB patients and were positively correlated with serum ALT. (a) Serum IL-8 and PBMCs IL-8 mRNA of 56 CHB patients and 40 healthy controls were assessed by ELISA and qRT-PCR. (b) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with serum ALT levels. (c) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with TBIL levels.

Mentions: To evaluate whether IL-8 protein and mRNA were increased in CHB, the levels of IL-8 protein and mRNA in CHB patients and healthy individuals were analyzed. As expected, IL-8 protein and mRNA were significantly higher in CHB patients than that of health controls (Figure 2(a)). We also investigated whether IL-8 expression was related with serum ALT in CHB patients. And the analysis results revealed that there was positive correlation between serum IL-8 and ALT levels (r = 0.569, p = 0.000) (Figure 2(b)). IL-8 mRNA in PBMCs was also positively correlated with serum ALT level (r = 0.645, p = 0.000) (Figure 2(b)). In addition, serum TBIL levels were found to be significantly correlated with IL-8 mRNA level (r = 0.498, p = 0.000) and serum IL-8 protein level (r = 0.476, p = 0.000) (Figure 2(c)).


miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8.

Hong Z, Hong H, Liu J, Zheng X, Huang M, Li C, Xia J - Mediators Inflamm. (2015)

Serum IL-8 protein and mRNA levels were significantly increased in CHB patients and were positively correlated with serum ALT. (a) Serum IL-8 and PBMCs IL-8 mRNA of 56 CHB patients and 40 healthy controls were assessed by ELISA and qRT-PCR. (b) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with serum ALT levels. (c) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with TBIL levels.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4525765&req=5

fig2: Serum IL-8 protein and mRNA levels were significantly increased in CHB patients and were positively correlated with serum ALT. (a) Serum IL-8 and PBMCs IL-8 mRNA of 56 CHB patients and 40 healthy controls were assessed by ELISA and qRT-PCR. (b) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with serum ALT levels. (c) Both serum IL-8 and PBMCs IL-8 mRNA were positively correlated with TBIL levels.
Mentions: To evaluate whether IL-8 protein and mRNA were increased in CHB, the levels of IL-8 protein and mRNA in CHB patients and healthy individuals were analyzed. As expected, IL-8 protein and mRNA were significantly higher in CHB patients than that of health controls (Figure 2(a)). We also investigated whether IL-8 expression was related with serum ALT in CHB patients. And the analysis results revealed that there was positive correlation between serum IL-8 and ALT levels (r = 0.569, p = 0.000) (Figure 2(b)). IL-8 mRNA in PBMCs was also positively correlated with serum ALT level (r = 0.645, p = 0.000) (Figure 2(b)). In addition, serum TBIL levels were found to be significantly correlated with IL-8 mRNA level (r = 0.498, p = 0.000) and serum IL-8 protein level (r = 0.476, p = 0.000) (Figure 2(c)).

Bottom Line: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China.

ABSTRACT

Aims: This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.

Materials and methods: miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3'UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.

Results: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.

Conclusions: This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

No MeSH data available.


Related in: MedlinePlus