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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8.

Hong Z, Hong H, Liu J, Zheng X, Huang M, Li C, Xia J - Mediators Inflamm. (2015)

Bottom Line: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China.

ABSTRACT

Aims: This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.

Materials and methods: miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3'UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.

Results: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.

Conclusions: This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

No MeSH data available.


Related in: MedlinePlus

miR-106a is downregulated in PBMCs of CHB patients and is negatively correlated with serum alanine aminotransferase (ALT) level. (a) The expression levels of miR-106a in PBMCs of 56 CHB patients and 40 healthy controls were determined by qRT-PCR. The expression levels were normalized to U6B. (b) PBMC miR-106a was negatively correlated with serum ALT levels. (c) PBMC miR-106a was negatively correlated with TBIL levels.
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fig1: miR-106a is downregulated in PBMCs of CHB patients and is negatively correlated with serum alanine aminotransferase (ALT) level. (a) The expression levels of miR-106a in PBMCs of 56 CHB patients and 40 healthy controls were determined by qRT-PCR. The expression levels were normalized to U6B. (b) PBMC miR-106a was negatively correlated with serum ALT levels. (c) PBMC miR-106a was negatively correlated with TBIL levels.

Mentions: To identify the miRNAs potentially associated with HBV infection, the expression levels of miR-106a from 56 CHB patients and 40 healthy controls were determined by qRT-PCR. The clinical data of 56 CHB patients are shown in Table 1. We observed that the miR-106a expression level was decreased in PBMCs from CHB patients compared with that in healthy controls (Figure 1(a)). Serum alanine aminotransferase (ALT) and TBIL have been widely recognized as an important marker of liver disease. Here, we analyzed the relationship between miR-106a expression, serum ALT, and TBIL level in CHB patients. The results showed that miR-106a expression was inversely proportional to serum ALT level (r = −0.693, p = 0.000) and TBIL level (r = −0.545, p = 0.000) (Figures 1(b) and 1(c)).


miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8.

Hong Z, Hong H, Liu J, Zheng X, Huang M, Li C, Xia J - Mediators Inflamm. (2015)

miR-106a is downregulated in PBMCs of CHB patients and is negatively correlated with serum alanine aminotransferase (ALT) level. (a) The expression levels of miR-106a in PBMCs of 56 CHB patients and 40 healthy controls were determined by qRT-PCR. The expression levels were normalized to U6B. (b) PBMC miR-106a was negatively correlated with serum ALT levels. (c) PBMC miR-106a was negatively correlated with TBIL levels.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4525765&req=5

fig1: miR-106a is downregulated in PBMCs of CHB patients and is negatively correlated with serum alanine aminotransferase (ALT) level. (a) The expression levels of miR-106a in PBMCs of 56 CHB patients and 40 healthy controls were determined by qRT-PCR. The expression levels were normalized to U6B. (b) PBMC miR-106a was negatively correlated with serum ALT levels. (c) PBMC miR-106a was negatively correlated with TBIL levels.
Mentions: To identify the miRNAs potentially associated with HBV infection, the expression levels of miR-106a from 56 CHB patients and 40 healthy controls were determined by qRT-PCR. The clinical data of 56 CHB patients are shown in Table 1. We observed that the miR-106a expression level was decreased in PBMCs from CHB patients compared with that in healthy controls (Figure 1(a)). Serum alanine aminotransferase (ALT) and TBIL have been widely recognized as an important marker of liver disease. Here, we analyzed the relationship between miR-106a expression, serum ALT, and TBIL level in CHB patients. The results showed that miR-106a expression was inversely proportional to serum ALT level (r = −0.693, p = 0.000) and TBIL level (r = −0.545, p = 0.000) (Figures 1(b) and 1(c)).

Bottom Line: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Disease, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China.

ABSTRACT

Aims: This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.

Materials and methods: miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3'UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.

Results: The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.

Conclusions: This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

No MeSH data available.


Related in: MedlinePlus