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Elevated body swing test after focal cerebral ischemia in rodents: methodological considerations.

Ingberg E, Gudjonsdottir J, Theodorsson E, Theodorsson A, Ström JO - BMC Neurosci (2015)

Bottom Line: Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations.The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology and Molecular Medicine, Department of Clinical and Experimental Medicine, Department of Clinical Chemistry, Faculty of Health Sciences, Center for Diagnostics, Linköping University, Region Östergötland, Linköping, Sweden. edvin.ingberg@liu.se.

ABSTRACT

Background: The elevated body swing test (EBST) is a behavioral test used to evaluate experimental stroke in rodents. The basic idea is that when the animal is suspended vertically by the tail, it will swing its head laterally to the left or right depending on lesion side. In a previous study from our lab using the EBST after middle cerebral artery occlusion (MCAo), rats swung contralateral to the infarct day 1 post-MCAo, but ipsilateral day 3 post-MCAo. This shift was unexpected and prompted us to perform the present study. First, the literature was systematically reviewed to elucidate whether a similar shift had been noticed before, and if consensus existed regarding swing direction. Secondly, an experiment was conducted to systematically investigate the suggested behavior. Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.

Results: Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations. The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

Conclusions: This study highlights the importance of adequate reporting of behavioral tests for lateralization and it is concluded that the EBST cannot be recommended as a test for motor asymmetry after MCAo in rats.

No MeSH data available.


Related in: MedlinePlus

EBST performance of rats displaying a shift. EBST performance of rats from MCAo groups (Male 30 min, Male 60 min, Male 90 min and Female 60 min) displaying a shift in direction (proportion of right-side swings >0.8 on day 1 and <0.4 on days 2 or 3).
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Fig6: EBST performance of rats displaying a shift. EBST performance of rats from MCAo groups (Male 30 min, Male 60 min, Male 90 min and Female 60 min) displaying a shift in direction (proportion of right-side swings >0.8 on day 1 and <0.4 on days 2 or 3).

Mentions: Even though no significant shifting behavior was seen on a group level, a separate graph was constructed to illustrate that the phenomenon existed among several individuals. From MCAo groups (Male 30 min, Male 60 min, Male 90 min and Female 60 min), individual rats with proportion of right-side swings >0.8 on day 1 and <0.4 on days 2 or 3 were plotted (Fig. 6). With this approach, 12/40 rats (30%) of the rats met the criteria (3 from Male 30 min, 5 from male 60 min, one from Male 90 min and 3 from Female 60 min). As comparison, when the criteria were inverted (proportion of right-side swings <0.2 on day 1 and >0.6 on days 2 or 3), only one rat was included.Fig. 6


Elevated body swing test after focal cerebral ischemia in rodents: methodological considerations.

Ingberg E, Gudjonsdottir J, Theodorsson E, Theodorsson A, Ström JO - BMC Neurosci (2015)

EBST performance of rats displaying a shift. EBST performance of rats from MCAo groups (Male 30 min, Male 60 min, Male 90 min and Female 60 min) displaying a shift in direction (proportion of right-side swings >0.8 on day 1 and <0.4 on days 2 or 3).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4525734&req=5

Fig6: EBST performance of rats displaying a shift. EBST performance of rats from MCAo groups (Male 30 min, Male 60 min, Male 90 min and Female 60 min) displaying a shift in direction (proportion of right-side swings >0.8 on day 1 and <0.4 on days 2 or 3).
Mentions: Even though no significant shifting behavior was seen on a group level, a separate graph was constructed to illustrate that the phenomenon existed among several individuals. From MCAo groups (Male 30 min, Male 60 min, Male 90 min and Female 60 min), individual rats with proportion of right-side swings >0.8 on day 1 and <0.4 on days 2 or 3 were plotted (Fig. 6). With this approach, 12/40 rats (30%) of the rats met the criteria (3 from Male 30 min, 5 from male 60 min, one from Male 90 min and 3 from Female 60 min). As comparison, when the criteria were inverted (proportion of right-side swings <0.2 on day 1 and >0.6 on days 2 or 3), only one rat was included.Fig. 6

Bottom Line: Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations.The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology and Molecular Medicine, Department of Clinical and Experimental Medicine, Department of Clinical Chemistry, Faculty of Health Sciences, Center for Diagnostics, Linköping University, Region Östergötland, Linköping, Sweden. edvin.ingberg@liu.se.

ABSTRACT

Background: The elevated body swing test (EBST) is a behavioral test used to evaluate experimental stroke in rodents. The basic idea is that when the animal is suspended vertically by the tail, it will swing its head laterally to the left or right depending on lesion side. In a previous study from our lab using the EBST after middle cerebral artery occlusion (MCAo), rats swung contralateral to the infarct day 1 post-MCAo, but ipsilateral day 3 post-MCAo. This shift was unexpected and prompted us to perform the present study. First, the literature was systematically reviewed to elucidate whether a similar shift had been noticed before, and if consensus existed regarding swing direction. Secondly, an experiment was conducted to systematically investigate the suggested behavior. Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.

Results: Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations. The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

Conclusions: This study highlights the importance of adequate reporting of behavioral tests for lateralization and it is concluded that the EBST cannot be recommended as a test for motor asymmetry after MCAo in rats.

No MeSH data available.


Related in: MedlinePlus