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Elevated body swing test after focal cerebral ischemia in rodents: methodological considerations.

Ingberg E, Gudjonsdottir J, Theodorsson E, Theodorsson A, Ström JO - BMC Neurosci (2015)

Bottom Line: Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations.The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology and Molecular Medicine, Department of Clinical and Experimental Medicine, Department of Clinical Chemistry, Faculty of Health Sciences, Center for Diagnostics, Linköping University, Region Östergötland, Linköping, Sweden. edvin.ingberg@liu.se.

ABSTRACT

Background: The elevated body swing test (EBST) is a behavioral test used to evaluate experimental stroke in rodents. The basic idea is that when the animal is suspended vertically by the tail, it will swing its head laterally to the left or right depending on lesion side. In a previous study from our lab using the EBST after middle cerebral artery occlusion (MCAo), rats swung contralateral to the infarct day 1 post-MCAo, but ipsilateral day 3 post-MCAo. This shift was unexpected and prompted us to perform the present study. First, the literature was systematically reviewed to elucidate whether a similar shift had been noticed before, and if consensus existed regarding swing direction. Secondly, an experiment was conducted to systematically investigate the suggested behavior. Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.

Results: Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations. The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

Conclusions: This study highlights the importance of adequate reporting of behavioral tests for lateralization and it is concluded that the EBST cannot be recommended as a test for motor asymmetry after MCAo in rats.

No MeSH data available.


Related in: MedlinePlus

Experimental outline. Fourteen days before MCAo the females were ovariectomized (day −14). On day 0 the animals were subjected to either MCAo or sham operation and on days 1, 2, 3 and 7 the EBST was performed. The animals were sacrificed on day 7.
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Fig3: Experimental outline. Fourteen days before MCAo the females were ovariectomized (day −14). On day 0 the animals were subjected to either MCAo or sham operation and on days 1, 2, 3 and 7 the EBST was performed. The animals were sacrificed on day 7.

Mentions: The rats were randomly allocated into six groups: Four MCAo groups (Male 30 min, n = 13; Male 60 min, n = 20; Male 90 min, n = 18; Female 60 min, n = 13) and two sham groups (Male sham ICA, n = 9; Male sham CCA + ECA, n = 10). See Fig. 3 for an overview of the experiment. Animals of both sexes, as well as different occlusion times were included to enable a broader investigation of EBST behavior.Fig. 3


Elevated body swing test after focal cerebral ischemia in rodents: methodological considerations.

Ingberg E, Gudjonsdottir J, Theodorsson E, Theodorsson A, Ström JO - BMC Neurosci (2015)

Experimental outline. Fourteen days before MCAo the females were ovariectomized (day −14). On day 0 the animals were subjected to either MCAo or sham operation and on days 1, 2, 3 and 7 the EBST was performed. The animals were sacrificed on day 7.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4525734&req=5

Fig3: Experimental outline. Fourteen days before MCAo the females were ovariectomized (day −14). On day 0 the animals were subjected to either MCAo or sham operation and on days 1, 2, 3 and 7 the EBST was performed. The animals were sacrificed on day 7.
Mentions: The rats were randomly allocated into six groups: Four MCAo groups (Male 30 min, n = 13; Male 60 min, n = 20; Male 90 min, n = 18; Female 60 min, n = 13) and two sham groups (Male sham ICA, n = 9; Male sham CCA + ECA, n = 10). See Fig. 3 for an overview of the experiment. Animals of both sexes, as well as different occlusion times were included to enable a broader investigation of EBST behavior.Fig. 3

Bottom Line: Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations.The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology and Molecular Medicine, Department of Clinical and Experimental Medicine, Department of Clinical Chemistry, Faculty of Health Sciences, Center for Diagnostics, Linköping University, Region Östergötland, Linköping, Sweden. edvin.ingberg@liu.se.

ABSTRACT

Background: The elevated body swing test (EBST) is a behavioral test used to evaluate experimental stroke in rodents. The basic idea is that when the animal is suspended vertically by the tail, it will swing its head laterally to the left or right depending on lesion side. In a previous study from our lab using the EBST after middle cerebral artery occlusion (MCAo), rats swung contralateral to the infarct day 1 post-MCAo, but ipsilateral day 3 post-MCAo. This shift was unexpected and prompted us to perform the present study. First, the literature was systematically reviewed to elucidate whether a similar shift had been noticed before, and if consensus existed regarding swing direction. Secondly, an experiment was conducted to systematically investigate the suggested behavior. Eighty-three adult male and female Sprague-Dawley rats were subjected to MCAo or sham surgery and the EBST was performed up to 7 days after the lesion.

Results: Both experimentally and through systematic literature review, the present study shows that the direction of biased swing activity in the EBST for rodents after cerebral ischemia can differ and even shift over time in some situations. The EBST curve for females was significantly different from that of males after the same occlusion time (p = 0.023).

Conclusions: This study highlights the importance of adequate reporting of behavioral tests for lateralization and it is concluded that the EBST cannot be recommended as a test for motor asymmetry after MCAo in rats.

No MeSH data available.


Related in: MedlinePlus