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Plasma membrane proteomic analysis of human Gastric Cancer tissues: revealing flotillin 1 as a marker for Gastric Cancer.

Gao W, Xu J, Wang F, Zhang L, Peng R, Shu Y, Wu J, Tang Q, Zhu Y - BMC Cancer (2015)

Bottom Line: Of a total data set that included 501 identified proteins, about 35% of the identified proteins were found to be plasma membrane and associated proteins.Additionally, several potential biomarkers participated in endocytosis pathway and integrin signaling pathways were firstly identified as differentially expressed proteins in GC samples.Together, the results show that subcellular proteomics of tumor tissue is a feasible and promising avenue for exploring oncogenesis.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Department of Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, 300 GuangZhou Road, Nanjing, 210029, China. Yoghurt831030@126.Com.

ABSTRACT

Background: Gastric cancer remains the second leading cause of cancer-related deaths in the world. Successful early gastric cancer detection is hampered by lack of highly sensitive and specific biomarkers. Plasma membrane proteins participate and/or have a central role in the metastatic process of cancer cells and are potentially useful for cancer therapy due to easy accessibility of the targets.

Methods: In the present research, TMT method followed by mass spectrometry analysis was used to compare the relative expression levels of plasma membrane proteins between noncancer and gastric cancer tissues.

Results: Of a total data set that included 501 identified proteins, about 35% of the identified proteins were found to be plasma membrane and associated proteins. Among them, 82 proteins were at least 1.5-fold up- or down-regulated in gastric cancer compared with the adherent normal tissues.

Conclusions: A number of markers (e.g. annexin A6, caveolin 1, epidermal growth factor receptor, integrin beta 4) were previously reported as biomarkers of GC. Additionally, several potential biomarkers participated in endocytosis pathway and integrin signaling pathways were firstly identified as differentially expressed proteins in GC samples. Our findings also supported the notion that flotillin 1 is a potential biomarker that could be exploited for molecular imaging-based detection of gastric cancer. Together, the results show that subcellular proteomics of tumor tissue is a feasible and promising avenue for exploring oncogenesis.

No MeSH data available.


Related in: MedlinePlus

Ingenuity Pathway Analysis of proteins that were significantly altered in pathways.
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Fig5: Ingenuity Pathway Analysis of proteins that were significantly altered in pathways.

Mentions: To better appreciate the molecular and functional characteristics of the 82 differentially expressed plasma membrane or membrane-associated proteins, these proteins were subjected to IPA analysis for further identification of important biological processes that they were significantly involved in. The over-represented biological processes, molecular functions, and canonical pathways were generated based on information contained in the Ingenuity Pathways Knowledge Base. We found that the top three significant biological processes of the differentially expressed proteins in our study were networks describing 1) cancer, renal and urological system development and function, tissue morphology; 2) cell-to-cell signaling and interaction, infectious disease, cellular function and maintenance; 3) cellular assembly and organization, nervous system development and function, cellular movement. For molecular and cellular functions, the data indicated that many proteins involved in cellular function and maintenance, cell-to-cell signaling and interaction, cell morphology and cellular movement. Our results showed that the top three canonical pathways of differentially expressed proteins participated in were virus entry via endocytic pathways, caveolar-mediated endocytosis signaling and integrin signaling (FigureĀ 5).Figure 5


Plasma membrane proteomic analysis of human Gastric Cancer tissues: revealing flotillin 1 as a marker for Gastric Cancer.

Gao W, Xu J, Wang F, Zhang L, Peng R, Shu Y, Wu J, Tang Q, Zhu Y - BMC Cancer (2015)

Ingenuity Pathway Analysis of proteins that were significantly altered in pathways.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4525731&req=5

Fig5: Ingenuity Pathway Analysis of proteins that were significantly altered in pathways.
Mentions: To better appreciate the molecular and functional characteristics of the 82 differentially expressed plasma membrane or membrane-associated proteins, these proteins were subjected to IPA analysis for further identification of important biological processes that they were significantly involved in. The over-represented biological processes, molecular functions, and canonical pathways were generated based on information contained in the Ingenuity Pathways Knowledge Base. We found that the top three significant biological processes of the differentially expressed proteins in our study were networks describing 1) cancer, renal and urological system development and function, tissue morphology; 2) cell-to-cell signaling and interaction, infectious disease, cellular function and maintenance; 3) cellular assembly and organization, nervous system development and function, cellular movement. For molecular and cellular functions, the data indicated that many proteins involved in cellular function and maintenance, cell-to-cell signaling and interaction, cell morphology and cellular movement. Our results showed that the top three canonical pathways of differentially expressed proteins participated in were virus entry via endocytic pathways, caveolar-mediated endocytosis signaling and integrin signaling (FigureĀ 5).Figure 5

Bottom Line: Of a total data set that included 501 identified proteins, about 35% of the identified proteins were found to be plasma membrane and associated proteins.Additionally, several potential biomarkers participated in endocytosis pathway and integrin signaling pathways were firstly identified as differentially expressed proteins in GC samples.Together, the results show that subcellular proteomics of tumor tissue is a feasible and promising avenue for exploring oncogenesis.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Department of Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, 300 GuangZhou Road, Nanjing, 210029, China. Yoghurt831030@126.Com.

ABSTRACT

Background: Gastric cancer remains the second leading cause of cancer-related deaths in the world. Successful early gastric cancer detection is hampered by lack of highly sensitive and specific biomarkers. Plasma membrane proteins participate and/or have a central role in the metastatic process of cancer cells and are potentially useful for cancer therapy due to easy accessibility of the targets.

Methods: In the present research, TMT method followed by mass spectrometry analysis was used to compare the relative expression levels of plasma membrane proteins between noncancer and gastric cancer tissues.

Results: Of a total data set that included 501 identified proteins, about 35% of the identified proteins were found to be plasma membrane and associated proteins. Among them, 82 proteins were at least 1.5-fold up- or down-regulated in gastric cancer compared with the adherent normal tissues.

Conclusions: A number of markers (e.g. annexin A6, caveolin 1, epidermal growth factor receptor, integrin beta 4) were previously reported as biomarkers of GC. Additionally, several potential biomarkers participated in endocytosis pathway and integrin signaling pathways were firstly identified as differentially expressed proteins in GC samples. Our findings also supported the notion that flotillin 1 is a potential biomarker that could be exploited for molecular imaging-based detection of gastric cancer. Together, the results show that subcellular proteomics of tumor tissue is a feasible and promising avenue for exploring oncogenesis.

No MeSH data available.


Related in: MedlinePlus