Limits...
Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling.

Mitchell CA, Long H, Donaldson M, Francese S, Clench MR - Lipids Health Dis (2015)

Bottom Line: In particular development of an epidermal layer was observable as a compaction of the distribution of phosphatidylcholine species.MSI can be used to study changes in lipid composition in LSE.Determination of the changes in lipid distribution during the maturation of the LSE will assist in the identification of treatment responses in future investigations.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB, UK. c.mitchell@surrey.ac.uk.

ABSTRACT

Background: Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents during their development and maturation is described.

Methods: Living skin equivalent (LSE) samples were obtained at 14 days development, re-suspended in maintenance medium and incubated for 24 h after delivery. The medium was then changed, the LSE re-incubated and samples taken at 4, 6 and 24 h time points. Mass spectra and mass spectral images were recorded from 12 μm sections of the LSE taken at each time point for comparison using matrix assisted laser desorption ionisation mass spectrometry.

Results: A large number of lipid species were identified in the LSE via accurate mass-measurement MS and MSMS experiments carried out directly on the tissue sections. MS images acquired at a spatial resolution of 50 μm × 50 μm showed the distribution of identified lipids within the developing LSE and changes in their distribution with time. In particular development of an epidermal layer was observable as a compaction of the distribution of phosphatidylcholine species.

Conclusions: MSI can be used to study changes in lipid composition in LSE. Determination of the changes in lipid distribution during the maturation of the LSE will assist in the identification of treatment responses in future investigations.

No MeSH data available.


MALDI-IMS-MSI images mapping the protonated and sodiated ions for ceramides and PC species across skin equivalent tissue sections representing the three time-stages within the time-course study (4, 6 or 24 h incubation period [from left to right]) and across lipid standards. Images are at a spatial resolution of 50 μm × 50 μm and are normalised against the total ion count
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4525729&req=5

Fig4: MALDI-IMS-MSI images mapping the protonated and sodiated ions for ceramides and PC species across skin equivalent tissue sections representing the three time-stages within the time-course study (4, 6 or 24 h incubation period [from left to right]) and across lipid standards. Images are at a spatial resolution of 50 μm × 50 μm and are normalised against the total ion count

Mentions: Figure 4 shows MALDI-MS images of identified lipid compounds in the tissue. Trigylcerol (TG) 54:3 (m/z 907.7566 [M + Na]+) is a marker of the stratum corneum; its distribution can be observed at the outer most regions of the skin sections, as reported also by Hart et al., [10]. The ceramide species when visualised appeared less intense generally in comparison to the PC lipid ion maps in the tissue. Some broad differences could be observed between the tissue sections (4, 6, 24 h) for most of the images. The distribution of the ceramide and PC ions in the 24 h sample appeared to be located primarily towards the top outer region of the tissue section, whereas in the 4 and 6 h skin sections, the signal is observed throughout the sections.Fig. 4


Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling.

Mitchell CA, Long H, Donaldson M, Francese S, Clench MR - Lipids Health Dis (2015)

MALDI-IMS-MSI images mapping the protonated and sodiated ions for ceramides and PC species across skin equivalent tissue sections representing the three time-stages within the time-course study (4, 6 or 24 h incubation period [from left to right]) and across lipid standards. Images are at a spatial resolution of 50 μm × 50 μm and are normalised against the total ion count
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4525729&req=5

Fig4: MALDI-IMS-MSI images mapping the protonated and sodiated ions for ceramides and PC species across skin equivalent tissue sections representing the three time-stages within the time-course study (4, 6 or 24 h incubation period [from left to right]) and across lipid standards. Images are at a spatial resolution of 50 μm × 50 μm and are normalised against the total ion count
Mentions: Figure 4 shows MALDI-MS images of identified lipid compounds in the tissue. Trigylcerol (TG) 54:3 (m/z 907.7566 [M + Na]+) is a marker of the stratum corneum; its distribution can be observed at the outer most regions of the skin sections, as reported also by Hart et al., [10]. The ceramide species when visualised appeared less intense generally in comparison to the PC lipid ion maps in the tissue. Some broad differences could be observed between the tissue sections (4, 6, 24 h) for most of the images. The distribution of the ceramide and PC ions in the 24 h sample appeared to be located primarily towards the top outer region of the tissue section, whereas in the 4 and 6 h skin sections, the signal is observed throughout the sections.Fig. 4

Bottom Line: In particular development of an epidermal layer was observable as a compaction of the distribution of phosphatidylcholine species.MSI can be used to study changes in lipid composition in LSE.Determination of the changes in lipid distribution during the maturation of the LSE will assist in the identification of treatment responses in future investigations.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB, UK. c.mitchell@surrey.ac.uk.

ABSTRACT

Background: Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents during their development and maturation is described.

Methods: Living skin equivalent (LSE) samples were obtained at 14 days development, re-suspended in maintenance medium and incubated for 24 h after delivery. The medium was then changed, the LSE re-incubated and samples taken at 4, 6 and 24 h time points. Mass spectra and mass spectral images were recorded from 12 μm sections of the LSE taken at each time point for comparison using matrix assisted laser desorption ionisation mass spectrometry.

Results: A large number of lipid species were identified in the LSE via accurate mass-measurement MS and MSMS experiments carried out directly on the tissue sections. MS images acquired at a spatial resolution of 50 μm × 50 μm showed the distribution of identified lipids within the developing LSE and changes in their distribution with time. In particular development of an epidermal layer was observable as a compaction of the distribution of phosphatidylcholine species.

Conclusions: MSI can be used to study changes in lipid composition in LSE. Determination of the changes in lipid distribution during the maturation of the LSE will assist in the identification of treatment responses in future investigations.

No MeSH data available.