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Distinct mechanical behavior of HEK293 cells in adherent and suspended states.

Haghparast SM, Kihara T, Miyake J - PeerJ (2015)

Bottom Line: The mechanical features of individual animal cells have been regarded as indicators of cell type and state.In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells.Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.

ABSTRACT
The mechanical features of individual animal cells have been regarded as indicators of cell type and state. Previously, we investigated the surface mechanics of cancer and normal stromal cells in adherent and suspended states using atomic force microscopy. Cancer cells possessed specific mechanical and actin cytoskeleton features that were distinct from normal stromal cells in adherent and suspended states. In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells. Unlike normal stromal and cancer cells, the surface stiffness of adherent HEK293 cells was very low, but increased after cell detachment from the culture surface. Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells. The exclusive mechanical response of HEK293 cells to perturbation of the actin cytoskeleton resembled that of adherent cancer cells and suspended normal stromal cells. Thus, with respect to their special cell-surface mechanical features, HEK293 cells could be categorized into a new class distinct from normal stromal and cancer cells.

No MeSH data available.


Related in: MedlinePlus

Elastic responses of adherent and BAM-anchored suspended cells following treatment with Y27632 and calyculin A.The distribution and logarithmic average of the Young’s modulus of HEK293 cells in adherent and suspended states are shown. Each condition shows the Young’s modulus of 20 individual cells.
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fig-4: Elastic responses of adherent and BAM-anchored suspended cells following treatment with Y27632 and calyculin A.The distribution and logarithmic average of the Young’s modulus of HEK293 cells in adherent and suspended states are shown. Each condition shows the Young’s modulus of 20 individual cells.

Mentions: In the adherent state, the Young’s modulus of the cell surface slightly decreased by treatment with Y27632, but increased by treatment with calyculin A (Fig. 4). On the other hand, in the suspended state, the Young’s modulus clearly decreased by addition of Y27632 and was almost unchanged after addition of calyculin A (Fig. 4). Thus, the mechanical responsiveness of surface F-actin of HEK293 cells differs depending on whether the cells are in adherent or suspended state.


Distinct mechanical behavior of HEK293 cells in adherent and suspended states.

Haghparast SM, Kihara T, Miyake J - PeerJ (2015)

Elastic responses of adherent and BAM-anchored suspended cells following treatment with Y27632 and calyculin A.The distribution and logarithmic average of the Young’s modulus of HEK293 cells in adherent and suspended states are shown. Each condition shows the Young’s modulus of 20 individual cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525692&req=5

fig-4: Elastic responses of adherent and BAM-anchored suspended cells following treatment with Y27632 and calyculin A.The distribution and logarithmic average of the Young’s modulus of HEK293 cells in adherent and suspended states are shown. Each condition shows the Young’s modulus of 20 individual cells.
Mentions: In the adherent state, the Young’s modulus of the cell surface slightly decreased by treatment with Y27632, but increased by treatment with calyculin A (Fig. 4). On the other hand, in the suspended state, the Young’s modulus clearly decreased by addition of Y27632 and was almost unchanged after addition of calyculin A (Fig. 4). Thus, the mechanical responsiveness of surface F-actin of HEK293 cells differs depending on whether the cells are in adherent or suspended state.

Bottom Line: The mechanical features of individual animal cells have been regarded as indicators of cell type and state.In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells.Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.

ABSTRACT
The mechanical features of individual animal cells have been regarded as indicators of cell type and state. Previously, we investigated the surface mechanics of cancer and normal stromal cells in adherent and suspended states using atomic force microscopy. Cancer cells possessed specific mechanical and actin cytoskeleton features that were distinct from normal stromal cells in adherent and suspended states. In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells. Unlike normal stromal and cancer cells, the surface stiffness of adherent HEK293 cells was very low, but increased after cell detachment from the culture surface. Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells. The exclusive mechanical response of HEK293 cells to perturbation of the actin cytoskeleton resembled that of adherent cancer cells and suspended normal stromal cells. Thus, with respect to their special cell-surface mechanical features, HEK293 cells could be categorized into a new class distinct from normal stromal and cancer cells.

No MeSH data available.


Related in: MedlinePlus