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Distinct mechanical behavior of HEK293 cells in adherent and suspended states.

Haghparast SM, Kihara T, Miyake J - PeerJ (2015)

Bottom Line: The mechanical features of individual animal cells have been regarded as indicators of cell type and state.In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells.Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.

ABSTRACT
The mechanical features of individual animal cells have been regarded as indicators of cell type and state. Previously, we investigated the surface mechanics of cancer and normal stromal cells in adherent and suspended states using atomic force microscopy. Cancer cells possessed specific mechanical and actin cytoskeleton features that were distinct from normal stromal cells in adherent and suspended states. In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells. Unlike normal stromal and cancer cells, the surface stiffness of adherent HEK293 cells was very low, but increased after cell detachment from the culture surface. Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells. The exclusive mechanical response of HEK293 cells to perturbation of the actin cytoskeleton resembled that of adherent cancer cells and suspended normal stromal cells. Thus, with respect to their special cell-surface mechanical features, HEK293 cells could be categorized into a new class distinct from normal stromal and cancer cells.

No MeSH data available.


Related in: MedlinePlus

Young’s modulus of HEK293 cells in the adherent and suspended states.The distributions of the Young’s moduli in adherent and suspended states are shown as box-and-whisker plots (Normal). The Young’s moduli of the cells treated with Cytochalasin D are shown on the right (Cytochalasin D). The logarithmic average of the Young’s moduli is shown on the top of each plot. Each condition shows the Young’s modulus of more than 20 individual cells.
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fig-3: Young’s modulus of HEK293 cells in the adherent and suspended states.The distributions of the Young’s moduli in adherent and suspended states are shown as box-and-whisker plots (Normal). The Young’s moduli of the cells treated with Cytochalasin D are shown on the right (Cytochalasin D). The logarithmic average of the Young’s moduli is shown on the top of each plot. Each condition shows the Young’s modulus of more than 20 individual cells.

Mentions: Figure 3 shows the distribution of the Young’s modulus of the cells in the two adhesion states. The Young’s modulus values were broadly distributed, irrespective of the cell adhesion state. The distribution of the Young’s moduli of suspended round cells was clearly higher than that of adherent cells (Fig. 3). In short, upon detachment from the substrate and development of a round transformed morphology, distribution of the Young’s modulus of HEK293 cell surface increased.


Distinct mechanical behavior of HEK293 cells in adherent and suspended states.

Haghparast SM, Kihara T, Miyake J - PeerJ (2015)

Young’s modulus of HEK293 cells in the adherent and suspended states.The distributions of the Young’s moduli in adherent and suspended states are shown as box-and-whisker plots (Normal). The Young’s moduli of the cells treated with Cytochalasin D are shown on the right (Cytochalasin D). The logarithmic average of the Young’s moduli is shown on the top of each plot. Each condition shows the Young’s modulus of more than 20 individual cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525692&req=5

fig-3: Young’s modulus of HEK293 cells in the adherent and suspended states.The distributions of the Young’s moduli in adherent and suspended states are shown as box-and-whisker plots (Normal). The Young’s moduli of the cells treated with Cytochalasin D are shown on the right (Cytochalasin D). The logarithmic average of the Young’s moduli is shown on the top of each plot. Each condition shows the Young’s modulus of more than 20 individual cells.
Mentions: Figure 3 shows the distribution of the Young’s modulus of the cells in the two adhesion states. The Young’s modulus values were broadly distributed, irrespective of the cell adhesion state. The distribution of the Young’s moduli of suspended round cells was clearly higher than that of adherent cells (Fig. 3). In short, upon detachment from the substrate and development of a round transformed morphology, distribution of the Young’s modulus of HEK293 cell surface increased.

Bottom Line: The mechanical features of individual animal cells have been regarded as indicators of cell type and state.In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells.Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.

ABSTRACT
The mechanical features of individual animal cells have been regarded as indicators of cell type and state. Previously, we investigated the surface mechanics of cancer and normal stromal cells in adherent and suspended states using atomic force microscopy. Cancer cells possessed specific mechanical and actin cytoskeleton features that were distinct from normal stromal cells in adherent and suspended states. In this paper, we report the unique mechanical and actin cytoskeletal features of human embryonic kidney HEK293 cells. Unlike normal stromal and cancer cells, the surface stiffness of adherent HEK293 cells was very low, but increased after cell detachment from the culture surface. Induced actin filament depolymerization revealed that the actin cytoskeleton was the underlying source of the stiffness in suspended HEK293 cells. The exclusive mechanical response of HEK293 cells to perturbation of the actin cytoskeleton resembled that of adherent cancer cells and suspended normal stromal cells. Thus, with respect to their special cell-surface mechanical features, HEK293 cells could be categorized into a new class distinct from normal stromal and cancer cells.

No MeSH data available.


Related in: MedlinePlus