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Protective Effect of Ethanolic Extract of Grape Pomace against the Adverse Effects of Cypermethrin on Weanling Female Rats.

Mossa AT, Ibrahim FM, Mohafrash SM, Abou Baker DH, El Gengaihi S - Evid Based Complement Alternat Med (2015)

Bottom Line: An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration.Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine.The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Chemical Industries Research Division, Pesticide Chemistry Department, National Research Centre, 33 El Bohouth Street (Former El Tahrir Street), P.O. Box 12622, Dokki, Giza, Egypt.

ABSTRACT
The adverse effect of cypermethrin on the liver and kidney of weanling female rats and the protective effect of ethanolic extract of grape pomace were investigated in the present study. Weanling female rats were given cypermethrin oral at a dose of 25 mg kg(-1) body weight for 28 consecutive days. An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration. Three groups more served as extract and control groups. Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine. In contrast, Cyp caused significant decrease in levels of total protein and albumin and caused histopathological alterations in liver and kidneys of female rats. Coadministration of the extract to Cyp-treated female rats restored most of these biochemical parameters to within normal levels especially at high dose of extract. However, extract administration to Cyp-treated rats resulted in overall improvement in liver and kidney damage. This study demonstrated the adverse biohistological effects of Cyp on the liver and kidney of weanling female rats. The grape pomace extract administration prevented the toxic effect of Cyp on the above serum parameters. The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

No MeSH data available.


Related in: MedlinePlus

Serum total protein (a), albumin (b), urea nitrogen (c), and creatinine (d) levels of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P ≤ 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and grape pomace extract at 100 mg/kg b.wt. (V), and cypermethrin and grape pomace extract at 200 mg/kg b.wt. (VI).
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fig3: Serum total protein (a), albumin (b), urea nitrogen (c), and creatinine (d) levels of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P ≤ 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and grape pomace extract at 100 mg/kg b.wt. (V), and cypermethrin and grape pomace extract at 200 mg/kg b.wt. (VI).

Mentions: The results demonstrating the level of liver and kidneys damage sustained following exposure to Cyp are shown in Figures 2 and 3. Significant increases in AST, ALT, ALP, and GGT activities were observed in Cyp-treated rats compared to the corresponding control values (Figure 2). Albumin and total protein levels were significantly decreased in Cyp-treated female rats, while urea nitrogen and creatinine levels showed a significant increase compared to the corresponding control values (Figure 3). However, supplementation of grape pomace extract at 200 mg/kg. b.wt. in conjunction with Cyp reversed the increase in AST, ALP, and GGT activities and urea nitrogen level and the decrease in albumin and total protein levels to within the normal limits. In contrast, ALT activity and creatinine level of Cyp-treated rats given 200 mg/kg. b.wt. grape pomace extract were still statistically different from the normal control values. Coadministration of grape pomace extract at 100 and 200 mg/kg. b.wt. along with Cyp-treated female rats improved liver and kidney functions in a dose-dependent manner. Supplementation of grape pomace extract at 100 and 200 mg/kg. b.wt. to female rats did not result in significant change in serum biomarkers of liver and kidney of female rats.


Protective Effect of Ethanolic Extract of Grape Pomace against the Adverse Effects of Cypermethrin on Weanling Female Rats.

Mossa AT, Ibrahim FM, Mohafrash SM, Abou Baker DH, El Gengaihi S - Evid Based Complement Alternat Med (2015)

Serum total protein (a), albumin (b), urea nitrogen (c), and creatinine (d) levels of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P ≤ 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and grape pomace extract at 100 mg/kg b.wt. (V), and cypermethrin and grape pomace extract at 200 mg/kg b.wt. (VI).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4525468&req=5

fig3: Serum total protein (a), albumin (b), urea nitrogen (c), and creatinine (d) levels of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P ≤ 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and grape pomace extract at 100 mg/kg b.wt. (V), and cypermethrin and grape pomace extract at 200 mg/kg b.wt. (VI).
Mentions: The results demonstrating the level of liver and kidneys damage sustained following exposure to Cyp are shown in Figures 2 and 3. Significant increases in AST, ALT, ALP, and GGT activities were observed in Cyp-treated rats compared to the corresponding control values (Figure 2). Albumin and total protein levels were significantly decreased in Cyp-treated female rats, while urea nitrogen and creatinine levels showed a significant increase compared to the corresponding control values (Figure 3). However, supplementation of grape pomace extract at 200 mg/kg. b.wt. in conjunction with Cyp reversed the increase in AST, ALP, and GGT activities and urea nitrogen level and the decrease in albumin and total protein levels to within the normal limits. In contrast, ALT activity and creatinine level of Cyp-treated rats given 200 mg/kg. b.wt. grape pomace extract were still statistically different from the normal control values. Coadministration of grape pomace extract at 100 and 200 mg/kg. b.wt. along with Cyp-treated female rats improved liver and kidney functions in a dose-dependent manner. Supplementation of grape pomace extract at 100 and 200 mg/kg. b.wt. to female rats did not result in significant change in serum biomarkers of liver and kidney of female rats.

Bottom Line: An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration.Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine.The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Chemical Industries Research Division, Pesticide Chemistry Department, National Research Centre, 33 El Bohouth Street (Former El Tahrir Street), P.O. Box 12622, Dokki, Giza, Egypt.

ABSTRACT
The adverse effect of cypermethrin on the liver and kidney of weanling female rats and the protective effect of ethanolic extract of grape pomace were investigated in the present study. Weanling female rats were given cypermethrin oral at a dose of 25 mg kg(-1) body weight for 28 consecutive days. An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration. Three groups more served as extract and control groups. Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine. In contrast, Cyp caused significant decrease in levels of total protein and albumin and caused histopathological alterations in liver and kidneys of female rats. Coadministration of the extract to Cyp-treated female rats restored most of these biochemical parameters to within normal levels especially at high dose of extract. However, extract administration to Cyp-treated rats resulted in overall improvement in liver and kidney damage. This study demonstrated the adverse biohistological effects of Cyp on the liver and kidney of weanling female rats. The grape pomace extract administration prevented the toxic effect of Cyp on the above serum parameters. The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

No MeSH data available.


Related in: MedlinePlus