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Protective Effect of Ethanolic Extract of Grape Pomace against the Adverse Effects of Cypermethrin on Weanling Female Rats.

Mossa AT, Ibrahim FM, Mohafrash SM, Abou Baker DH, El Gengaihi S - Evid Based Complement Alternat Med (2015)

Bottom Line: An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration.Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine.The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Chemical Industries Research Division, Pesticide Chemistry Department, National Research Centre, 33 El Bohouth Street (Former El Tahrir Street), P.O. Box 12622, Dokki, Giza, Egypt.

ABSTRACT
The adverse effect of cypermethrin on the liver and kidney of weanling female rats and the protective effect of ethanolic extract of grape pomace were investigated in the present study. Weanling female rats were given cypermethrin oral at a dose of 25 mg kg(-1) body weight for 28 consecutive days. An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration. Three groups more served as extract and control groups. Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine. In contrast, Cyp caused significant decrease in levels of total protein and albumin and caused histopathological alterations in liver and kidneys of female rats. Coadministration of the extract to Cyp-treated female rats restored most of these biochemical parameters to within normal levels especially at high dose of extract. However, extract administration to Cyp-treated rats resulted in overall improvement in liver and kidney damage. This study demonstrated the adverse biohistological effects of Cyp on the liver and kidney of weanling female rats. The grape pomace extract administration prevented the toxic effect of Cyp on the above serum parameters. The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

No MeSH data available.


Related in: MedlinePlus

Percent of weekly body weight gain (a) and relative liver (b) and kidney (c) weights of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P < 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and extract at 100 mg/kg b.wt. (V), and cypermethrin and extract at 100 mg/kg b.wt. (VI). Percent of weekly body weight gain = (initial b.wt. − final b.wt./no. of weeks) × 100; relative liver and kidney weight = (liver or kidney weight (g)/final b.wt. (g)) × 100.
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fig1: Percent of weekly body weight gain (a) and relative liver (b) and kidney (c) weights of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P < 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and extract at 100 mg/kg b.wt. (V), and cypermethrin and extract at 100 mg/kg b.wt. (VI). Percent of weekly body weight gain = (initial b.wt. − final b.wt./no. of weeks) × 100; relative liver and kidney weight = (liver or kidney weight (g)/final b.wt. (g)) × 100.

Mentions: Results of weekly body weight gain and relative organ weights of female rats are shown in Figure 1. Results revealed that Cyp-treated female rats induced significant decrease in weekly body weight gain compared to control (Figure 1(a)). Administration of grape pomace extract at 200 mg/kg. b.wt. to Cyp-treated female rats showed insignificant differences in body weight gains, while body weight gain of Cyp-treated rats given 100 mg/kg. b.wt. grape pomace extract was still statistically different from the normal control values. The relative weights of the liver and kidney were significantly changed of Cyp-treated female rats compared to untreated rats (Figures 1(b) and 1(c)). Supplementation of grape pomace extract at 100 and 200 mg/kg. b.wt. to Cyp-treated female rats showed no significant differences in relative weights of liver and kidney as compared to control. Treatment with grape pomace extract at 100 and 200 mg/kg. b.wt. alone did not result in significant change in body and relative organ weights compared to control.


Protective Effect of Ethanolic Extract of Grape Pomace against the Adverse Effects of Cypermethrin on Weanling Female Rats.

Mossa AT, Ibrahim FM, Mohafrash SM, Abou Baker DH, El Gengaihi S - Evid Based Complement Alternat Med (2015)

Percent of weekly body weight gain (a) and relative liver (b) and kidney (c) weights of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P < 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and extract at 100 mg/kg b.wt. (V), and cypermethrin and extract at 100 mg/kg b.wt. (VI). Percent of weekly body weight gain = (initial b.wt. − final b.wt./no. of weeks) × 100; relative liver and kidney weight = (liver or kidney weight (g)/final b.wt. (g)) × 100.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4525468&req=5

fig1: Percent of weekly body weight gain (a) and relative liver (b) and kidney (c) weights of weanling female rats exposed to cypermethrin for 28 days and the protective effect of ethanolic extract of grape pomace (100 and 200 mg/kg b.wt.). The values represented are the means ± S.D. Means having the same letters are not significantly different from each other, P < 0.05. Groups: control (I), grape pomace extract at 100 mg/kg b.wt. (II), grape pomace extract at 200 mg/kg b.wt. (III), cypermethrin (IV), cypermethrin and extract at 100 mg/kg b.wt. (V), and cypermethrin and extract at 100 mg/kg b.wt. (VI). Percent of weekly body weight gain = (initial b.wt. − final b.wt./no. of weeks) × 100; relative liver and kidney weight = (liver or kidney weight (g)/final b.wt. (g)) × 100.
Mentions: Results of weekly body weight gain and relative organ weights of female rats are shown in Figure 1. Results revealed that Cyp-treated female rats induced significant decrease in weekly body weight gain compared to control (Figure 1(a)). Administration of grape pomace extract at 200 mg/kg. b.wt. to Cyp-treated female rats showed insignificant differences in body weight gains, while body weight gain of Cyp-treated rats given 100 mg/kg. b.wt. grape pomace extract was still statistically different from the normal control values. The relative weights of the liver and kidney were significantly changed of Cyp-treated female rats compared to untreated rats (Figures 1(b) and 1(c)). Supplementation of grape pomace extract at 100 and 200 mg/kg. b.wt. to Cyp-treated female rats showed no significant differences in relative weights of liver and kidney as compared to control. Treatment with grape pomace extract at 100 and 200 mg/kg. b.wt. alone did not result in significant change in body and relative organ weights compared to control.

Bottom Line: An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration.Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine.The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Chemical Industries Research Division, Pesticide Chemistry Department, National Research Centre, 33 El Bohouth Street (Former El Tahrir Street), P.O. Box 12622, Dokki, Giza, Egypt.

ABSTRACT
The adverse effect of cypermethrin on the liver and kidney of weanling female rats and the protective effect of ethanolic extract of grape pomace were investigated in the present study. Weanling female rats were given cypermethrin oral at a dose of 25 mg kg(-1) body weight for 28 consecutive days. An additional two Cyp-trated groups received extract at a dose of 100 and 200 mg kg(-1) body weight, respectively, throughout the experimental duration. Three groups more served as extract and control groups. Administration of Cyp resulted in a significant increase in serum marker enzymes, for example, aminotransferases (AST and ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), and increases the level of urea nitrogen and creatinine. In contrast, Cyp caused significant decrease in levels of total protein and albumin and caused histopathological alterations in liver and kidneys of female rats. Coadministration of the extract to Cyp-treated female rats restored most of these biochemical parameters to within normal levels especially at high dose of extract. However, extract administration to Cyp-treated rats resulted in overall improvement in liver and kidney damage. This study demonstrated the adverse biohistological effects of Cyp on the liver and kidney of weanling female rats. The grape pomace extract administration prevented the toxic effect of Cyp on the above serum parameters. The present study concludes that grape pomace extract has significant antioxidant and hepatorenal protective activity.

No MeSH data available.


Related in: MedlinePlus