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Effect of Fluoride-Modified Titanium Surface on Early Adhesion of Irradiated Osteoblasts.

Li JY, Zheng LW, Ma L, Kwong DL, Cheung LK, Pow EH - Biomed Res Int (2015)

Bottom Line: The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface.Meanwhile, the cells on the fluoride-modified surface formed more actin filaments.Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.

ABSTRACT

Objective: The present study aimed to investigate the effect of fluoride-modified titanium surface on adhesion of irradiated osteoblasts.

Materials and methods: Fluoride-modified surface was obtained and the morphology, roughness, and chemical composition of the surface were evaluated by scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy, respectively. The adhesion of irradiated osteoblast-like cells, in terms of number, area, and fluorescence intensity on the titanium surface, was evaluated using immunofluorescence staining.

Results: Numerous nanosize pits were seen only in the F-TiO surface. The pits were more remarkable and uniform on F-TiO surface than on TiO surface; however, the amplitude of peaks and bottoms on F-TiO surface appeared to be smaller than on TiO surface. The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface. The concentrations of main elements such as titanium, oxygen, and carbon were similar on both surfaces. The number of irradiated osteoblasts adhered on the control surface was larger than on fluoride-modified surface. Meanwhile, the cells on the fluoride-modified surface formed more actin filaments.

Conclusions: The fluoride-modified titanium surface alters the adhesion of irradiated osteoblasts. Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface.

No MeSH data available.


Related in: MedlinePlus

Electron radiation of cells in the water tank.
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Related In: Results  -  Collection


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fig1: Electron radiation of cells in the water tank.

Mentions: The cells were radiated with a linear accelerator radiotherapeutic machine (Varian, Palo Alto, CA, USA) in Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong. Cells were harvested with density of 2 × 104 cell/mL, collected in 1 mL tubes, and stored in the icebox. In order to ensure uniform radiation distribution, the tubes containing cells were placed in the water tank, and electron radiation rays went through the center of tubes (Figure 1). The dose rate was 400 cgy/min. The cells received 0 Gy, 2 Gy, 4 Gy, 6 Gy, 8 Gy, or 10 Gy radiation respectively.


Effect of Fluoride-Modified Titanium Surface on Early Adhesion of Irradiated Osteoblasts.

Li JY, Zheng LW, Ma L, Kwong DL, Cheung LK, Pow EH - Biomed Res Int (2015)

Electron radiation of cells in the water tank.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4525467&req=5

fig1: Electron radiation of cells in the water tank.
Mentions: The cells were radiated with a linear accelerator radiotherapeutic machine (Varian, Palo Alto, CA, USA) in Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong. Cells were harvested with density of 2 × 104 cell/mL, collected in 1 mL tubes, and stored in the icebox. In order to ensure uniform radiation distribution, the tubes containing cells were placed in the water tank, and electron radiation rays went through the center of tubes (Figure 1). The dose rate was 400 cgy/min. The cells received 0 Gy, 2 Gy, 4 Gy, 6 Gy, 8 Gy, or 10 Gy radiation respectively.

Bottom Line: The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface.Meanwhile, the cells on the fluoride-modified surface formed more actin filaments.Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.

ABSTRACT

Objective: The present study aimed to investigate the effect of fluoride-modified titanium surface on adhesion of irradiated osteoblasts.

Materials and methods: Fluoride-modified surface was obtained and the morphology, roughness, and chemical composition of the surface were evaluated by scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy, respectively. The adhesion of irradiated osteoblast-like cells, in terms of number, area, and fluorescence intensity on the titanium surface, was evaluated using immunofluorescence staining.

Results: Numerous nanosize pits were seen only in the F-TiO surface. The pits were more remarkable and uniform on F-TiO surface than on TiO surface; however, the amplitude of peaks and bottoms on F-TiO surface appeared to be smaller than on TiO surface. The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface. The concentrations of main elements such as titanium, oxygen, and carbon were similar on both surfaces. The number of irradiated osteoblasts adhered on the control surface was larger than on fluoride-modified surface. Meanwhile, the cells on the fluoride-modified surface formed more actin filaments.

Conclusions: The fluoride-modified titanium surface alters the adhesion of irradiated osteoblasts. Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface.

No MeSH data available.


Related in: MedlinePlus