Limits...
Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy.

Wang J, Lin K, Zheng W, Ho KY, Teh M, Yeoh KG, Huang Z - Sci Rep (2015)

Bottom Line: A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination.The total Raman dataset was split into two parts: 80% for training; while 20% for testing.Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification.

View Article: PubMed Central - PubMed

Affiliation: Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117576.

ABSTRACT
This work aims to evaluate clinical value of a fiber-optic Raman spectroscopy technique developed for in vivo diagnosis of esophageal squamous cell carcinoma (ESCC) during clinical endoscopy. We have developed a rapid fiber-optic Raman endoscopic system capable of simultaneously acquiring both fingerprint (FP)(800-1800 cm(-1)) and high-wavenumber (HW)(2800-3600 cm(-1)) Raman spectra from esophageal tissue in vivo. A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination. The total Raman dataset was split into two parts: 80% for training; while 20% for testing. Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification. PLS-DA-LOPCV shows that simultaneous FP/HW Raman spectroscopy on training dataset provides a diagnostic sensitivity of 97.0% and specificity of 97.4% for ESCC classification. Further, the diagnostic algorithm applied to the independent testing dataset based on simultaneous FP/HW Raman technique gives a predictive diagnostic sensitivity of 92.7% and specificity of 93.6% for ESCC identification, which is superior to either FP or HW Raman technique alone. This work demonstrates that the simultaneous FP/HW fiber-optic Raman spectroscopy technique improves real-time in vivo diagnosis of esophageal neoplasia at endoscopy.

No MeSH data available.


Related in: MedlinePlus

(a) Unpaired two-sided Student’s t-test on Raman peakintensities of the training dataset (80% of the total dataset) (normal(n = 736); ESCC (n = 202))over the entire spectral range (i.e.,800–1800 cm−1 and2800–3600 cm−1).Seven Raman spectra sub-regions containing the diagnostically significantinformation were identified. (b)Histogram ± 1 SD of the mostdiagnostically significant Raman peaks(*p < 1E-10).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4525386&req=5

f2: (a) Unpaired two-sided Student’s t-test on Raman peakintensities of the training dataset (80% of the total dataset) (normal(n = 736); ESCC (n = 202))over the entire spectral range (i.e.,800–1800 cm−1 and2800–3600 cm−1).Seven Raman spectra sub-regions containing the diagnostically significantinformation were identified. (b)Histogram ± 1 SD of the mostdiagnostically significant Raman peaks(*p < 1E-10).

Mentions: To elucidate the diagnostically important Raman-active components, Fig. 2(a) shows a logarithmic plot of the calculated p-values(unpaired two-sided Student’s t-test) for each of the Raman intensitiesin the entire spectral range (i.e.,800–1800 cm−1 and2800–3600 cm−1). Inparticular, seven spectral sub-regions with statically significant difference(p < 1E-10) between ESCC and normal esophagus werefound: i.e., 840–940 cm−1,1025–1100 cm−1,1310–1355 cm−1,1585–1690 cm−1, and2830–2975 cm−1 related toproteins, lipids and nucleic acids. Significant spectral differences were alsoobserved in bound water in the ranges of3160–3260 cm−1 and3370–3420 cm−1. Figure 2(b) displays a histogram of the most statisticallydifferent Raman peak intensities (mean ± 1SD)for both the FP and HW ranges, i.e., (i)853 cm−1, (ii)1078 cm−1, (iii)1335 cm−1, (iv)1618 cm−1, (v)1655 cm−1, (vi)2850 cm−1, (vii)2885 cm−1, (viii)3250 cm−1, and (ix)3400 cm−1. The histopathology identifiesprominent cellular and architectural anomalies in ESCC (Fig.3), while the relatively higher or lower FP/HW tissue Raman bandsrepresenting different Raman-active components reveal the specificbiochemical/biomolecular changes of esophageal tissue accompanied with ESCCtransformation. The changes of FP/HW Raman spectra related to lipids, proteins, DNAand water contents in tissue reconfirm the capability of simultaneous FP/HW Ramanspectroscopy to detect ESCC at the molecular level.


Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy.

Wang J, Lin K, Zheng W, Ho KY, Teh M, Yeoh KG, Huang Z - Sci Rep (2015)

(a) Unpaired two-sided Student’s t-test on Raman peakintensities of the training dataset (80% of the total dataset) (normal(n = 736); ESCC (n = 202))over the entire spectral range (i.e.,800–1800 cm−1 and2800–3600 cm−1).Seven Raman spectra sub-regions containing the diagnostically significantinformation were identified. (b)Histogram ± 1 SD of the mostdiagnostically significant Raman peaks(*p < 1E-10).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525386&req=5

f2: (a) Unpaired two-sided Student’s t-test on Raman peakintensities of the training dataset (80% of the total dataset) (normal(n = 736); ESCC (n = 202))over the entire spectral range (i.e.,800–1800 cm−1 and2800–3600 cm−1).Seven Raman spectra sub-regions containing the diagnostically significantinformation were identified. (b)Histogram ± 1 SD of the mostdiagnostically significant Raman peaks(*p < 1E-10).
Mentions: To elucidate the diagnostically important Raman-active components, Fig. 2(a) shows a logarithmic plot of the calculated p-values(unpaired two-sided Student’s t-test) for each of the Raman intensitiesin the entire spectral range (i.e.,800–1800 cm−1 and2800–3600 cm−1). Inparticular, seven spectral sub-regions with statically significant difference(p < 1E-10) between ESCC and normal esophagus werefound: i.e., 840–940 cm−1,1025–1100 cm−1,1310–1355 cm−1,1585–1690 cm−1, and2830–2975 cm−1 related toproteins, lipids and nucleic acids. Significant spectral differences were alsoobserved in bound water in the ranges of3160–3260 cm−1 and3370–3420 cm−1. Figure 2(b) displays a histogram of the most statisticallydifferent Raman peak intensities (mean ± 1SD)for both the FP and HW ranges, i.e., (i)853 cm−1, (ii)1078 cm−1, (iii)1335 cm−1, (iv)1618 cm−1, (v)1655 cm−1, (vi)2850 cm−1, (vii)2885 cm−1, (viii)3250 cm−1, and (ix)3400 cm−1. The histopathology identifiesprominent cellular and architectural anomalies in ESCC (Fig.3), while the relatively higher or lower FP/HW tissue Raman bandsrepresenting different Raman-active components reveal the specificbiochemical/biomolecular changes of esophageal tissue accompanied with ESCCtransformation. The changes of FP/HW Raman spectra related to lipids, proteins, DNAand water contents in tissue reconfirm the capability of simultaneous FP/HW Ramanspectroscopy to detect ESCC at the molecular level.

Bottom Line: A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination.The total Raman dataset was split into two parts: 80% for training; while 20% for testing.Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification.

View Article: PubMed Central - PubMed

Affiliation: Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117576.

ABSTRACT
This work aims to evaluate clinical value of a fiber-optic Raman spectroscopy technique developed for in vivo diagnosis of esophageal squamous cell carcinoma (ESCC) during clinical endoscopy. We have developed a rapid fiber-optic Raman endoscopic system capable of simultaneously acquiring both fingerprint (FP)(800-1800 cm(-1)) and high-wavenumber (HW)(2800-3600 cm(-1)) Raman spectra from esophageal tissue in vivo. A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination. The total Raman dataset was split into two parts: 80% for training; while 20% for testing. Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification. PLS-DA-LOPCV shows that simultaneous FP/HW Raman spectroscopy on training dataset provides a diagnostic sensitivity of 97.0% and specificity of 97.4% for ESCC classification. Further, the diagnostic algorithm applied to the independent testing dataset based on simultaneous FP/HW Raman technique gives a predictive diagnostic sensitivity of 92.7% and specificity of 93.6% for ESCC identification, which is superior to either FP or HW Raman technique alone. This work demonstrates that the simultaneous FP/HW fiber-optic Raman spectroscopy technique improves real-time in vivo diagnosis of esophageal neoplasia at endoscopy.

No MeSH data available.


Related in: MedlinePlus