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Genome-wide identification of sweet orange (Citrus sinensis) histone modification gene families and their expression analysis during the fruit development and fruit-blue mold infection process.

Xu J, Xu H, Liu Y, Wang X, Xu Q, Deng X - Front Plant Sci (2015)

Bottom Line: In order to gain an insight into the potential roles of these genes in citrus fruit development, 42 CsHMs with high mRNA abundance in fruit tissues were selected to further analyze their expression profiles at six stages of fruit development.Interestingly, a numbers of genes were expressed highly in flesh of ripening fruit and some of them showed the increasing expression levels along with the fruit development.In conclusion, this study presents a comprehensive analysis of the histone modification gene families in sweet orange and further elucidates their behaviors during the fruit development and the blue mold infection responses.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University Wuhan, China.

ABSTRACT
In eukaryotes, histone acetylation and methylation have been known to be involved in regulating diverse developmental processes and plant defense. These histone modification events are controlled by a series of histone modification gene families. To date, there is no study regarding genome-wide characterization of histone modification related genes in citrus species. Based on the two recent sequenced sweet orange genome databases, a total of 136 CsHMs (Citrus sinensis histone modification genes), including 47 CsHMTs (histone methyltransferase genes), 23 CsHDMs (histone demethylase genes), 50 CsHATs (histone acetyltransferase genes), and 16 CsHDACs (histone deacetylase genes) were identified. These genes were categorized to 11 gene families. A comprehensive analysis of these 11 gene families was performed with chromosome locations, phylogenetic comparison, gene structures, and conserved domain compositions of proteins. In order to gain an insight into the potential roles of these genes in citrus fruit development, 42 CsHMs with high mRNA abundance in fruit tissues were selected to further analyze their expression profiles at six stages of fruit development. Interestingly, a numbers of genes were expressed highly in flesh of ripening fruit and some of them showed the increasing expression levels along with the fruit development. Furthermore, we analyzed the expression patterns of all 136 CsHMs response to the infection of blue mold (Penicillium digitatum), which is the most devastating pathogen in citrus post-harvest process. The results indicated that 20 of them showed the strong alterations of their expression levels during the fruit-pathogen infection. In conclusion, this study presents a comprehensive analysis of the histone modification gene families in sweet orange and further elucidates their behaviors during the fruit development and the blue mold infection responses.

No MeSH data available.


Related in: MedlinePlus

Chromosomal localization of CsHM genes.
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Figure 1: Chromosomal localization of CsHM genes.

Mentions: The chromosomal locations of CsHMs were demonstrated on sweet orange chromosome available at CAP8. The members of CsSDG family were widely distributed in eight chromosomes with no distribution in the ninth chromosome (Figure 1). The largest number of CsSDGs was located on chromosome 5 (seven CsSDGs). However, eight genes including CsSDG6, 21, 22, 34, 37, 38, 39, and 40 were not determined because the physical map of sweet orange was incomplete. CsPRMTs were distributed at chromosomes 4, 5, 7, and 9 (Figure 1). As regard CsHDMAs, CsHDMA2 was located in chromosome, while CsHDMA1 and CsHDMA3 were closely located in chromosome 3, suggesting the occurrence of tandem duplication. CsJMJs were widely distributed at chromosomes 2, 3, 5, 6, 7, and 8 and six of CsJMJs were located in chromosome 5.


Genome-wide identification of sweet orange (Citrus sinensis) histone modification gene families and their expression analysis during the fruit development and fruit-blue mold infection process.

Xu J, Xu H, Liu Y, Wang X, Xu Q, Deng X - Front Plant Sci (2015)

Chromosomal localization of CsHM genes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525380&req=5

Figure 1: Chromosomal localization of CsHM genes.
Mentions: The chromosomal locations of CsHMs were demonstrated on sweet orange chromosome available at CAP8. The members of CsSDG family were widely distributed in eight chromosomes with no distribution in the ninth chromosome (Figure 1). The largest number of CsSDGs was located on chromosome 5 (seven CsSDGs). However, eight genes including CsSDG6, 21, 22, 34, 37, 38, 39, and 40 were not determined because the physical map of sweet orange was incomplete. CsPRMTs were distributed at chromosomes 4, 5, 7, and 9 (Figure 1). As regard CsHDMAs, CsHDMA2 was located in chromosome, while CsHDMA1 and CsHDMA3 were closely located in chromosome 3, suggesting the occurrence of tandem duplication. CsJMJs were widely distributed at chromosomes 2, 3, 5, 6, 7, and 8 and six of CsJMJs were located in chromosome 5.

Bottom Line: In order to gain an insight into the potential roles of these genes in citrus fruit development, 42 CsHMs with high mRNA abundance in fruit tissues were selected to further analyze their expression profiles at six stages of fruit development.Interestingly, a numbers of genes were expressed highly in flesh of ripening fruit and some of them showed the increasing expression levels along with the fruit development.In conclusion, this study presents a comprehensive analysis of the histone modification gene families in sweet orange and further elucidates their behaviors during the fruit development and the blue mold infection responses.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University Wuhan, China.

ABSTRACT
In eukaryotes, histone acetylation and methylation have been known to be involved in regulating diverse developmental processes and plant defense. These histone modification events are controlled by a series of histone modification gene families. To date, there is no study regarding genome-wide characterization of histone modification related genes in citrus species. Based on the two recent sequenced sweet orange genome databases, a total of 136 CsHMs (Citrus sinensis histone modification genes), including 47 CsHMTs (histone methyltransferase genes), 23 CsHDMs (histone demethylase genes), 50 CsHATs (histone acetyltransferase genes), and 16 CsHDACs (histone deacetylase genes) were identified. These genes were categorized to 11 gene families. A comprehensive analysis of these 11 gene families was performed with chromosome locations, phylogenetic comparison, gene structures, and conserved domain compositions of proteins. In order to gain an insight into the potential roles of these genes in citrus fruit development, 42 CsHMs with high mRNA abundance in fruit tissues were selected to further analyze their expression profiles at six stages of fruit development. Interestingly, a numbers of genes were expressed highly in flesh of ripening fruit and some of them showed the increasing expression levels along with the fruit development. Furthermore, we analyzed the expression patterns of all 136 CsHMs response to the infection of blue mold (Penicillium digitatum), which is the most devastating pathogen in citrus post-harvest process. The results indicated that 20 of them showed the strong alterations of their expression levels during the fruit-pathogen infection. In conclusion, this study presents a comprehensive analysis of the histone modification gene families in sweet orange and further elucidates their behaviors during the fruit development and the blue mold infection responses.

No MeSH data available.


Related in: MedlinePlus