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Acute exposure to silica nanoparticles aggravate airway inflammation: different effects according to surface characteristics.

Park HJ, Sohn JH, Kim YJ, Park YH, Han H, Park KH, Lee K, Choi H, Um K, Choi IH, Park JW, Lee JH - Exp. Mol. Med. (2015)

Bottom Line: In OVA/SNPs asthma models, OVA with SNP-treated group showed significant airway inflammation, more than those treated with only OVA and without SNPs.Interleukin (IL)-5, IL-13, IL-1β and interferon-γ levels correlated with airway inflammation in the tested models, without statistical significance.P-SNPs appear to be relatively safer for clinical use than S-SNPs and M-SNPs, as determined by lower observed toxicity and airway system inflammation.

View Article: PubMed Central - PubMed

Affiliation: Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Severance Hospital, Seoul, Korea.

ABSTRACT
Silica nanoparticles (SNPs) are widely used in many scientific and industrial fields despite the lack of proper evaluation of their potential toxicity. This study examined the effects of acute exposure to SNPs, either alone or in conjunction with ovalbumin (OVA), by studying the respiratory systems in exposed mouse models. Three types of SNPs were used: spherical SNPs (S-SNPs), mesoporous SNPs (M-SNPs), and PEGylated SNPs (P-SNPs). In the acute SNP exposure model performed, 6-week-old BALB/c female mice were intranasally inoculated with SNPs for 3 consecutive days. In the OVA/SNPs asthma model, the mice were sensitized two times via the peritoneal route with OVA. Additionally, the mice endured OVA with or without SNP challenges intranasally. Acute SNP exposure induced significant airway inflammation and airway hyper-responsiveness, particularly in the S-SNP group. In OVA/SNPs asthma models, OVA with SNP-treated group showed significant airway inflammation, more than those treated with only OVA and without SNPs. In these models, the P-SNP group induced lower levels of inflammation on airways than both the S-SNP or M-SNP groups. Interleukin (IL)-5, IL-13, IL-1β and interferon-γ levels correlated with airway inflammation in the tested models, without statistical significance. In the mouse models studied, increased airway inflammation was associated with acute SNPs exposure, whether exposed solely to SNPs or SNPs in conjunction with OVA. P-SNPs appear to be relatively safer for clinical use than S-SNPs and M-SNPs, as determined by lower observed toxicity and airway system inflammation.

No MeSH data available.


Related in: MedlinePlus

Airway inflammation as determined by analysis of (a) bronchoalveolar lavage fluid (BALF) and (b) airway hyper-responsiveness in OVA/SNPs asthma models. *P<0.05 between two variables; #P<0.05 compared with others. M-SNP, mesoporous silica nanoparticle; OVA, ovalbumin; P-SNP, PEGylated silica nanoparticle; RL, Resistance of lung; S-SNP, spherical silica nanoparticle.
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fig6: Airway inflammation as determined by analysis of (a) bronchoalveolar lavage fluid (BALF) and (b) airway hyper-responsiveness in OVA/SNPs asthma models. *P<0.05 between two variables; #P<0.05 compared with others. M-SNP, mesoporous silica nanoparticle; OVA, ovalbumin; P-SNP, PEGylated silica nanoparticle; RL, Resistance of lung; S-SNP, spherical silica nanoparticle.

Mentions: SNPs, inoculated thrice in conjunction with OVA, induced asthma in the mice. Upon examination of the BALF, the total cell counts in the S-SNP and M-SNP groups were significantly higher than that in the control group. The number of macrophages was significantly higher in the S-SNP and M-SNP groups than in all of the other groups—the control group, OVA-only asthma group and P-SNP group. Eosinophils were significantly elevated in the S-SNP and M-SNP groups than in the control group. The total number of cells and eosinophils were higher in the P-SNP group than in the control group, but lower compared with those of the S-SNP and M-SNP groups (Figure 6a).


Acute exposure to silica nanoparticles aggravate airway inflammation: different effects according to surface characteristics.

Park HJ, Sohn JH, Kim YJ, Park YH, Han H, Park KH, Lee K, Choi H, Um K, Choi IH, Park JW, Lee JH - Exp. Mol. Med. (2015)

Airway inflammation as determined by analysis of (a) bronchoalveolar lavage fluid (BALF) and (b) airway hyper-responsiveness in OVA/SNPs asthma models. *P<0.05 between two variables; #P<0.05 compared with others. M-SNP, mesoporous silica nanoparticle; OVA, ovalbumin; P-SNP, PEGylated silica nanoparticle; RL, Resistance of lung; S-SNP, spherical silica nanoparticle.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525300&req=5

fig6: Airway inflammation as determined by analysis of (a) bronchoalveolar lavage fluid (BALF) and (b) airway hyper-responsiveness in OVA/SNPs asthma models. *P<0.05 between two variables; #P<0.05 compared with others. M-SNP, mesoporous silica nanoparticle; OVA, ovalbumin; P-SNP, PEGylated silica nanoparticle; RL, Resistance of lung; S-SNP, spherical silica nanoparticle.
Mentions: SNPs, inoculated thrice in conjunction with OVA, induced asthma in the mice. Upon examination of the BALF, the total cell counts in the S-SNP and M-SNP groups were significantly higher than that in the control group. The number of macrophages was significantly higher in the S-SNP and M-SNP groups than in all of the other groups—the control group, OVA-only asthma group and P-SNP group. Eosinophils were significantly elevated in the S-SNP and M-SNP groups than in the control group. The total number of cells and eosinophils were higher in the P-SNP group than in the control group, but lower compared with those of the S-SNP and M-SNP groups (Figure 6a).

Bottom Line: In OVA/SNPs asthma models, OVA with SNP-treated group showed significant airway inflammation, more than those treated with only OVA and without SNPs.Interleukin (IL)-5, IL-13, IL-1β and interferon-γ levels correlated with airway inflammation in the tested models, without statistical significance.P-SNPs appear to be relatively safer for clinical use than S-SNPs and M-SNPs, as determined by lower observed toxicity and airway system inflammation.

View Article: PubMed Central - PubMed

Affiliation: Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Severance Hospital, Seoul, Korea.

ABSTRACT
Silica nanoparticles (SNPs) are widely used in many scientific and industrial fields despite the lack of proper evaluation of their potential toxicity. This study examined the effects of acute exposure to SNPs, either alone or in conjunction with ovalbumin (OVA), by studying the respiratory systems in exposed mouse models. Three types of SNPs were used: spherical SNPs (S-SNPs), mesoporous SNPs (M-SNPs), and PEGylated SNPs (P-SNPs). In the acute SNP exposure model performed, 6-week-old BALB/c female mice were intranasally inoculated with SNPs for 3 consecutive days. In the OVA/SNPs asthma model, the mice were sensitized two times via the peritoneal route with OVA. Additionally, the mice endured OVA with or without SNP challenges intranasally. Acute SNP exposure induced significant airway inflammation and airway hyper-responsiveness, particularly in the S-SNP group. In OVA/SNPs asthma models, OVA with SNP-treated group showed significant airway inflammation, more than those treated with only OVA and without SNPs. In these models, the P-SNP group induced lower levels of inflammation on airways than both the S-SNP or M-SNP groups. Interleukin (IL)-5, IL-13, IL-1β and interferon-γ levels correlated with airway inflammation in the tested models, without statistical significance. In the mouse models studied, increased airway inflammation was associated with acute SNPs exposure, whether exposed solely to SNPs or SNPs in conjunction with OVA. P-SNPs appear to be relatively safer for clinical use than S-SNPs and M-SNPs, as determined by lower observed toxicity and airway system inflammation.

No MeSH data available.


Related in: MedlinePlus