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Manipulating the banana rhizosphere microbiome for biological control of Panama disease.

Xue C, Penton CR, Shen Z, Zhang R, Huang Q, Li R, Ruan Y, Shen Q - Sci Rep (2015)

Bottom Line: Results showed that BIO significantly decreased disease incidence by 68.5%, resulting in a doubled yield.Moreover, bacterial community structure was significantly correlated to disease incidence and yield and Bacillus colonization was negatively correlated with pathogen abundance and disease incidence, but positively correlated to yield.In total, the application of BIO altered the rhizo-bacterial community by establishing beneficial strains that dominated the microbial community and decreased pathogen colonization in the banana rhizosphere, which plays an important role in the management of Panama disease.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.

ABSTRACT
Panama disease caused by Fusarium oxysporum f. sp. cubense infection on banana is devastating banana plantations worldwide. Biological control has been proposed to suppress Panama disease, though the stability and survival of bio-control microorganisms in field setting is largely unknown. In order to develop a bio-control strategy for this disease, 16S rRNA gene sequencing was used to assess the microbial community of a disease-suppressive soil. Bacillus was identified as the dominant bacterial group in the suppressive soil. For this reason, B. amyloliquefaciens NJN-6 isolated from the suppressive soil was selected as a potential bio-control agent. A bioorganic fertilizer (BIO), formulated by combining this isolate with compost, was applied in nursery pots to assess the bio-control of Panama disease. Results showed that BIO significantly decreased disease incidence by 68.5%, resulting in a doubled yield. Moreover, bacterial community structure was significantly correlated to disease incidence and yield and Bacillus colonization was negatively correlated with pathogen abundance and disease incidence, but positively correlated to yield. In total, the application of BIO altered the rhizo-bacterial community by establishing beneficial strains that dominated the microbial community and decreased pathogen colonization in the banana rhizosphere, which plays an important role in the management of Panama disease.

No MeSH data available.


Related in: MedlinePlus

Non-metric multidimensional scaling analysis based on Bray-Curtis dissimilarity between all sample sets.CKH and CKD are healthy and diseased samples collected in control treatment, respectively. BIOH and BIOD are healthy and diseased samples collected in bioorganic fertilizer treatment, respectively. BIO and CK are samples collected in bioorganic fertilizer and control treatments, respectively, regardless of plant health status.
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f5: Non-metric multidimensional scaling analysis based on Bray-Curtis dissimilarity between all sample sets.CKH and CKD are healthy and diseased samples collected in control treatment, respectively. BIOH and BIOD are healthy and diseased samples collected in bioorganic fertilizer treatment, respectively. BIO and CK are samples collected in bioorganic fertilizer and control treatments, respectively, regardless of plant health status.

Mentions: Ordinations illustrated that all samples could be separated according to both health status and treatment (Fig. 5). PERMANOVA showed significant differences according to treatment (F = 2.66, p < 0.05), health status (F = 1.60, p < 0.05) and the treatment × health status interaction term (F = 1.67, p < 0.05). Overall sample dispersion was not significant (PERMDISP; p > 0.05). Multiple regression tree analyses resulted in a primary division of the bacterial community according to treatment, followed by health status (Fig. 6). Acidobacteria-Gp6 contributed most to the community differences between the healthy soil after BIO application and diseased soil in the control treatment, (SIMPER) followed by Gemmatimonas, Ohtaekwangia, Acidobacteria-Gp1, Steroidobacter, Bacillus, Streptophyta, Flavobacterium, and Dongia (Table S2). Overall bacterial community composition was significantly correlated to disease incidence (Mantel; r = 0.51, p < 0.05), banana unit yield (r = 0.60, p < 0.05) and plot yield (r = 0.54, p < 0.05).


Manipulating the banana rhizosphere microbiome for biological control of Panama disease.

Xue C, Penton CR, Shen Z, Zhang R, Huang Q, Li R, Ruan Y, Shen Q - Sci Rep (2015)

Non-metric multidimensional scaling analysis based on Bray-Curtis dissimilarity between all sample sets.CKH and CKD are healthy and diseased samples collected in control treatment, respectively. BIOH and BIOD are healthy and diseased samples collected in bioorganic fertilizer treatment, respectively. BIO and CK are samples collected in bioorganic fertilizer and control treatments, respectively, regardless of plant health status.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525139&req=5

f5: Non-metric multidimensional scaling analysis based on Bray-Curtis dissimilarity between all sample sets.CKH and CKD are healthy and diseased samples collected in control treatment, respectively. BIOH and BIOD are healthy and diseased samples collected in bioorganic fertilizer treatment, respectively. BIO and CK are samples collected in bioorganic fertilizer and control treatments, respectively, regardless of plant health status.
Mentions: Ordinations illustrated that all samples could be separated according to both health status and treatment (Fig. 5). PERMANOVA showed significant differences according to treatment (F = 2.66, p < 0.05), health status (F = 1.60, p < 0.05) and the treatment × health status interaction term (F = 1.67, p < 0.05). Overall sample dispersion was not significant (PERMDISP; p > 0.05). Multiple regression tree analyses resulted in a primary division of the bacterial community according to treatment, followed by health status (Fig. 6). Acidobacteria-Gp6 contributed most to the community differences between the healthy soil after BIO application and diseased soil in the control treatment, (SIMPER) followed by Gemmatimonas, Ohtaekwangia, Acidobacteria-Gp1, Steroidobacter, Bacillus, Streptophyta, Flavobacterium, and Dongia (Table S2). Overall bacterial community composition was significantly correlated to disease incidence (Mantel; r = 0.51, p < 0.05), banana unit yield (r = 0.60, p < 0.05) and plot yield (r = 0.54, p < 0.05).

Bottom Line: Results showed that BIO significantly decreased disease incidence by 68.5%, resulting in a doubled yield.Moreover, bacterial community structure was significantly correlated to disease incidence and yield and Bacillus colonization was negatively correlated with pathogen abundance and disease incidence, but positively correlated to yield.In total, the application of BIO altered the rhizo-bacterial community by establishing beneficial strains that dominated the microbial community and decreased pathogen colonization in the banana rhizosphere, which plays an important role in the management of Panama disease.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, PR China.

ABSTRACT
Panama disease caused by Fusarium oxysporum f. sp. cubense infection on banana is devastating banana plantations worldwide. Biological control has been proposed to suppress Panama disease, though the stability and survival of bio-control microorganisms in field setting is largely unknown. In order to develop a bio-control strategy for this disease, 16S rRNA gene sequencing was used to assess the microbial community of a disease-suppressive soil. Bacillus was identified as the dominant bacterial group in the suppressive soil. For this reason, B. amyloliquefaciens NJN-6 isolated from the suppressive soil was selected as a potential bio-control agent. A bioorganic fertilizer (BIO), formulated by combining this isolate with compost, was applied in nursery pots to assess the bio-control of Panama disease. Results showed that BIO significantly decreased disease incidence by 68.5%, resulting in a doubled yield. Moreover, bacterial community structure was significantly correlated to disease incidence and yield and Bacillus colonization was negatively correlated with pathogen abundance and disease incidence, but positively correlated to yield. In total, the application of BIO altered the rhizo-bacterial community by establishing beneficial strains that dominated the microbial community and decreased pathogen colonization in the banana rhizosphere, which plays an important role in the management of Panama disease.

No MeSH data available.


Related in: MedlinePlus