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Neuron hemilineages provide the functional ground plan for the Drosophila ventral nervous system.

Harris RM, Pfeiffer BD, Rubin GM, Truman JW - Elife (2015)

Bottom Line: The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking.The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff.These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

View Article: PubMed Central - PubMed

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

ABSTRACT
Drosophila central neurons arise from neuroblasts that generate neurons in a pair-wise fashion, with the two daughters providing the basis for distinct A and B hemilineage groups. 33 postembryonically-born hemilineages contribute over 90% of the neurons in each thoracic hemisegment. We devised genetic approaches to define the anatomy of most of these hemilineages and to assessed their functional roles using the heat-sensitive channel dTRPA1. The simplest hemilineages contained local interneurons and their activation caused tonic or phasic leg movements lacking interlimb coordination. The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking. The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff. These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

No MeSH data available.


Related in: MedlinePlus

tsh-GAL80 eliminates expression specifically in the VNC.(A) The lineage 11 genotype drives GFP expression in the VNC in lineage 11 (yellow arrowheads, arrows) and occasionally in descending axons (*). (B) The same genotype as in (A), but with the addition of tsh-GAL80. Expression is eliminated in lineage 11, but remains in the descending neurons. Genotypes A: nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/+; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005). (B) nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/tsh-GAL80; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005).DOI:http://dx.doi.org/10.7554/eLife.04493.030
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fig6s1: tsh-GAL80 eliminates expression specifically in the VNC.(A) The lineage 11 genotype drives GFP expression in the VNC in lineage 11 (yellow arrowheads, arrows) and occasionally in descending axons (*). (B) The same genotype as in (A), but with the addition of tsh-GAL80. Expression is eliminated in lineage 11, but remains in the descending neurons. Genotypes A: nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/+; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005). (B) nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/tsh-GAL80; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005).DOI:http://dx.doi.org/10.7554/eLife.04493.030

Mentions: Similar to the 7B interneurons, the activation of the lineage 11 interneurons using R26B05-GAL4 evoked takeoff behavior. High-speed video analysis showed that the behavioral sequencing differed from normal takeoff in that the decapitated flies commenced wing flapping prior to the jump (Figure 6F, Video 13). The R26B05 line used to activate the lineage 11 cells occasionally showed expression in a bundle of descending interneurons (Figure 6—figure supplement 1A) and we were concerned that their severed axons might be responsible for driving the takeoff behavior in the decapitated flies. Consequently, we used teashirt-GAL80 (tsh-GAL80; Clyne and Miesenböck, 2008) to suppress thoracic expression, but leaving expression in the descending interneurons (Figure 6—figure supplement 1B). These decapitated flies showed a severe reduction in the number of takeoffs when subjected to the heat ramp (9% of tsh-GAL80 flies [N = 22] vs 100% [N = 13] for R26B05-GAL4 flies lacking the tsh-GAL80).Video 13.High-speed video showing the behavioral effect of exciting the neurons in hemilineages 11A and B.


Neuron hemilineages provide the functional ground plan for the Drosophila ventral nervous system.

Harris RM, Pfeiffer BD, Rubin GM, Truman JW - Elife (2015)

tsh-GAL80 eliminates expression specifically in the VNC.(A) The lineage 11 genotype drives GFP expression in the VNC in lineage 11 (yellow arrowheads, arrows) and occasionally in descending axons (*). (B) The same genotype as in (A), but with the addition of tsh-GAL80. Expression is eliminated in lineage 11, but remains in the descending neurons. Genotypes A: nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/+; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005). (B) nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/tsh-GAL80; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005).DOI:http://dx.doi.org/10.7554/eLife.04493.030
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4525104&req=5

fig6s1: tsh-GAL80 eliminates expression specifically in the VNC.(A) The lineage 11 genotype drives GFP expression in the VNC in lineage 11 (yellow arrowheads, arrows) and occasionally in descending axons (*). (B) The same genotype as in (A), but with the addition of tsh-GAL80. Expression is eliminated in lineage 11, but remains in the descending neurons. Genotypes A: nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/+; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005). (B) nSyb-GAL80 (su(Hw)attP8)/w; UAS-flp(attP40)/tsh-GAL80; R26B05-GAL4 (attP2)/nSyb-LexA (attP2), LexAop>STOP>GFP (VK00005).DOI:http://dx.doi.org/10.7554/eLife.04493.030
Mentions: Similar to the 7B interneurons, the activation of the lineage 11 interneurons using R26B05-GAL4 evoked takeoff behavior. High-speed video analysis showed that the behavioral sequencing differed from normal takeoff in that the decapitated flies commenced wing flapping prior to the jump (Figure 6F, Video 13). The R26B05 line used to activate the lineage 11 cells occasionally showed expression in a bundle of descending interneurons (Figure 6—figure supplement 1A) and we were concerned that their severed axons might be responsible for driving the takeoff behavior in the decapitated flies. Consequently, we used teashirt-GAL80 (tsh-GAL80; Clyne and Miesenböck, 2008) to suppress thoracic expression, but leaving expression in the descending interneurons (Figure 6—figure supplement 1B). These decapitated flies showed a severe reduction in the number of takeoffs when subjected to the heat ramp (9% of tsh-GAL80 flies [N = 22] vs 100% [N = 13] for R26B05-GAL4 flies lacking the tsh-GAL80).Video 13.High-speed video showing the behavioral effect of exciting the neurons in hemilineages 11A and B.

Bottom Line: The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking.The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff.These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

View Article: PubMed Central - PubMed

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

ABSTRACT
Drosophila central neurons arise from neuroblasts that generate neurons in a pair-wise fashion, with the two daughters providing the basis for distinct A and B hemilineage groups. 33 postembryonically-born hemilineages contribute over 90% of the neurons in each thoracic hemisegment. We devised genetic approaches to define the anatomy of most of these hemilineages and to assessed their functional roles using the heat-sensitive channel dTRPA1. The simplest hemilineages contained local interneurons and their activation caused tonic or phasic leg movements lacking interlimb coordination. The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking. The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff. These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

No MeSH data available.


Related in: MedlinePlus