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Neuron hemilineages provide the functional ground plan for the Drosophila ventral nervous system.

Harris RM, Pfeiffer BD, Rubin GM, Truman JW - Elife (2015)

Bottom Line: The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking.The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff.These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

View Article: PubMed Central - PubMed

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

ABSTRACT
Drosophila central neurons arise from neuroblasts that generate neurons in a pair-wise fashion, with the two daughters providing the basis for distinct A and B hemilineage groups. 33 postembryonically-born hemilineages contribute over 90% of the neurons in each thoracic hemisegment. We devised genetic approaches to define the anatomy of most of these hemilineages and to assessed their functional roles using the heat-sensitive channel dTRPA1. The simplest hemilineages contained local interneurons and their activation caused tonic or phasic leg movements lacking interlimb coordination. The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking. The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff. These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

No MeSH data available.


Related in: MedlinePlus

UAS-hPR-flp is fully active in the presence of RU486, but inactive without drug.(A) R20B05-GAL4 drives UAS-GFP expression in all secondary neurons. (B) A larva bearing the genotype pJFRC2-UAS>STOP>GFP(attP18)/w; +; UAS-hPR-flp(VK00005)/R20B05-GAL4 and fed on RU486 food for 24 hr prior to dissection. GFP expression is present in nearly all of the expected cells, indicating that the STOP cassette has been excised by UAS-hPR-flp with high efficiency. (C) A larva of the same genotype as (B), but never treated with RU486. No GFP expression is observed, meaning that the hPR-flp was inactive in the absence of drug.DOI:http://dx.doi.org/10.7554/eLife.04493.006
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fig3s1: UAS-hPR-flp is fully active in the presence of RU486, but inactive without drug.(A) R20B05-GAL4 drives UAS-GFP expression in all secondary neurons. (B) A larva bearing the genotype pJFRC2-UAS>STOP>GFP(attP18)/w; +; UAS-hPR-flp(VK00005)/R20B05-GAL4 and fed on RU486 food for 24 hr prior to dissection. GFP expression is present in nearly all of the expected cells, indicating that the STOP cassette has been excised by UAS-hPR-flp with high efficiency. (C) A larva of the same genotype as (B), but never treated with RU486. No GFP expression is observed, meaning that the hPR-flp was inactive in the absence of drug.DOI:http://dx.doi.org/10.7554/eLife.04493.006

Mentions: To make a more precise tool (depicted in Figure 1C), we fused the codon-optimized ligand-binding domain of the human progesterone receptor to the Flp recombinase, making recombinase activity dependent on the presence of RU486 (Figure 3F). Figure 3 (Figure 3—figure supplement 1) shows a test of this construct, pJFRC108-20XUAS-IVS-hPR::Flp-p10, which we call UAS-Flp-Switch. Expression of GFP in larval nervous systems carrying R20B05-GAL4, pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP (Nern et al., 2011), and the UAS-Flp-Switch showed very strong expression that was conditional on feeding larvae on RU486-containing food during the third larval instar (Harris, 2012). Using the Flp-Switch system in conjunction with GAL80 suppression (Figure 1C), we could then obtain clean expression in hemilineage 12B cells in the adult (Figure 3F).


Neuron hemilineages provide the functional ground plan for the Drosophila ventral nervous system.

Harris RM, Pfeiffer BD, Rubin GM, Truman JW - Elife (2015)

UAS-hPR-flp is fully active in the presence of RU486, but inactive without drug.(A) R20B05-GAL4 drives UAS-GFP expression in all secondary neurons. (B) A larva bearing the genotype pJFRC2-UAS>STOP>GFP(attP18)/w; +; UAS-hPR-flp(VK00005)/R20B05-GAL4 and fed on RU486 food for 24 hr prior to dissection. GFP expression is present in nearly all of the expected cells, indicating that the STOP cassette has been excised by UAS-hPR-flp with high efficiency. (C) A larva of the same genotype as (B), but never treated with RU486. No GFP expression is observed, meaning that the hPR-flp was inactive in the absence of drug.DOI:http://dx.doi.org/10.7554/eLife.04493.006
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525104&req=5

fig3s1: UAS-hPR-flp is fully active in the presence of RU486, but inactive without drug.(A) R20B05-GAL4 drives UAS-GFP expression in all secondary neurons. (B) A larva bearing the genotype pJFRC2-UAS>STOP>GFP(attP18)/w; +; UAS-hPR-flp(VK00005)/R20B05-GAL4 and fed on RU486 food for 24 hr prior to dissection. GFP expression is present in nearly all of the expected cells, indicating that the STOP cassette has been excised by UAS-hPR-flp with high efficiency. (C) A larva of the same genotype as (B), but never treated with RU486. No GFP expression is observed, meaning that the hPR-flp was inactive in the absence of drug.DOI:http://dx.doi.org/10.7554/eLife.04493.006
Mentions: To make a more precise tool (depicted in Figure 1C), we fused the codon-optimized ligand-binding domain of the human progesterone receptor to the Flp recombinase, making recombinase activity dependent on the presence of RU486 (Figure 3F). Figure 3 (Figure 3—figure supplement 1) shows a test of this construct, pJFRC108-20XUAS-IVS-hPR::Flp-p10, which we call UAS-Flp-Switch. Expression of GFP in larval nervous systems carrying R20B05-GAL4, pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP (Nern et al., 2011), and the UAS-Flp-Switch showed very strong expression that was conditional on feeding larvae on RU486-containing food during the third larval instar (Harris, 2012). Using the Flp-Switch system in conjunction with GAL80 suppression (Figure 1C), we could then obtain clean expression in hemilineage 12B cells in the adult (Figure 3F).

Bottom Line: The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking.The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff.These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

View Article: PubMed Central - PubMed

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

ABSTRACT
Drosophila central neurons arise from neuroblasts that generate neurons in a pair-wise fashion, with the two daughters providing the basis for distinct A and B hemilineage groups. 33 postembryonically-born hemilineages contribute over 90% of the neurons in each thoracic hemisegment. We devised genetic approaches to define the anatomy of most of these hemilineages and to assessed their functional roles using the heat-sensitive channel dTRPA1. The simplest hemilineages contained local interneurons and their activation caused tonic or phasic leg movements lacking interlimb coordination. The next level was hemilineages of similar projection cells that drove intersegmentally coordinated behaviors such as walking. The highest level involved hemilineages whose activation elicited complex behaviors such as takeoff. These activation phenotypes indicate that the hemilineages vary in their behavioral roles with some contributing to local networks for sensorimotor processing and others having higher order functions of coordinating these local networks into complex behavior.

No MeSH data available.


Related in: MedlinePlus