Defining the roles of arrestin2 and arrestin3 in vasoconstrictor receptor desensitization in hypertension.
Bottom Line: Desensitization of UTP-stimulated vessel contractions was increased in 12-wk-old (but not 6-wk-old) SHR animals.In conclusion, arrestin2 and 3 expression is elevated in resistance arteries during the emergence of the early hypertensive phenotype, which underlies an enhanced ability to desensitize vasoconstrictor signaling and vessel contraction.Such regulatory changes may act to compensate for increased vasoconstrictor-induced vessel contraction.
Affiliation: Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom; and email@example.com.Show MeSH
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Mentions: Western blotting analysis indicated that arrestin2 or arrestin3 siRNA treatments induced isoform-specific knockdowns of ≥85% and ≥80%, respectively, in both WKY- and SHR-derived cells (Fig. 7). Following MSMC transfection with negative-control siRNA, R2/R1 ratios in both WKY- and SHR-derived cells (Fig. 8, A, B, and E) were similar to those obtained in MSMC not transfected with siRNAs (see Fig. 6).
Affiliation: Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom; and firstname.lastname@example.org.