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Dietary Mannan Oligosaccharides: Counteracting the Side Effects of Soybean Meal Oil Inclusion on European Sea Bass (Dicentrarchus labrax) Gut Health and Skin Mucosa Mucus Production?

Torrecillas S, Montero D, Caballero MJ, Pittman KA, Custódio M, Campo A, Sweetman J, Izquierdo M - Front Immunol (2015)

Bottom Line: There are no effects of dietary oil or MOS in the skin mucosal patterns.Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut-associated immune system (GALT)-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth.However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Investigación en Acuicultura (GIA), Universidad de Las Palmas de Gran Canaria , Las Palmas de Gran Canaria , Spain.

ABSTRACT
The main objective of this study was to assess the effects of 4 g kg(-1) dietary mannan oligosaccharides (MOS) inclusion in soybean oil (SBO)- and fish oil (FO)-based diets on the gut health and skin mucosa mucus production of European sea bass juveniles after 8 weeks of feeding. Dietary MOS, regardless of the oil source, promoted growth. The intestinal somatic index was not affected, however dietary SBO reduced the intestinal fold length, while dietary MOS increased it. The dietary oil source fed produced changes on the posterior intestine fatty acid profiles irrespective of MOS dietary supplementation. SBO down-regulated the gene expression of TCRβ, COX2, IL-1β, TNFα, IL-8, IL-6, IL-10, TGFβ, and Ig and up-regulated MHCII. MOS supplementation up-regulated the expression of MHCI, CD4, COX2, TNFα, and Ig when included in FO-based diets. However, there was a minor up-regulating effect on these genes when MOS was supplemented in the SBO-based diet. Both dietary oil sources and MOS affected mean mucous cell areas within the posterior gut, however the addition of MOS to a SBO diet increased the mucous cell size over the values shown in FO fed fish. Dietary SBO also trends to reduce mucous cell density in the anterior gut relative to FO, suggesting a lower overall mucosal secretion. There are no effects of dietary oil or MOS in the skin mucosal patterns. Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut-associated immune system (GALT)-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth. However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis. This denotes the importance of a balanced dietary n-3/n-6 ratio for an appropriate GALT-immune response against MOS in European sea bass juveniles.

No MeSH data available.


Related in: MedlinePlus

Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO- and MOS-based diet (SBOMOS) after 60 days of supplementation.
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Figure 1: Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO- and MOS-based diet (SBOMOS) after 60 days of supplementation.

Mentions: Morphological evaluation of the posterior gut sections revealed an intact epithelial barrier, organized villi, lack of debris in the lumen and no signs of edema or vasodilatation for all dietary treatments (Figure 1). Two-way ANOVA analyses revealed the effect on fold length of dietary oil source [F(1,8) = 96.25, P < 0.05] and MOS [F(1,8) = 52.57, P < 0.05], as well as the interaction between both factors [F(1,8) = 23.31, P < 0.05] (Table 6; Figure 1). Indeed, fish fed FOMOS diet had the longest intestinal folds and fish fed SBO diet had the shortest when compared with the rest of the groups. There was no effect (P>0.05, n = 250/tank) of MOS or dietary oil source or their combination on the mucosal folds width, although a trend to wider folds was observed in fish fed FOMOS diet (Table 6).


Dietary Mannan Oligosaccharides: Counteracting the Side Effects of Soybean Meal Oil Inclusion on European Sea Bass (Dicentrarchus labrax) Gut Health and Skin Mucosa Mucus Production?

Torrecillas S, Montero D, Caballero MJ, Pittman KA, Custódio M, Campo A, Sweetman J, Izquierdo M - Front Immunol (2015)

Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO- and MOS-based diet (SBOMOS) after 60 days of supplementation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4525062&req=5

Figure 1: Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO- and MOS-based diet (SBOMOS) after 60 days of supplementation.
Mentions: Morphological evaluation of the posterior gut sections revealed an intact epithelial barrier, organized villi, lack of debris in the lumen and no signs of edema or vasodilatation for all dietary treatments (Figure 1). Two-way ANOVA analyses revealed the effect on fold length of dietary oil source [F(1,8) = 96.25, P < 0.05] and MOS [F(1,8) = 52.57, P < 0.05], as well as the interaction between both factors [F(1,8) = 23.31, P < 0.05] (Table 6; Figure 1). Indeed, fish fed FOMOS diet had the longest intestinal folds and fish fed SBO diet had the shortest when compared with the rest of the groups. There was no effect (P>0.05, n = 250/tank) of MOS or dietary oil source or their combination on the mucosal folds width, although a trend to wider folds was observed in fish fed FOMOS diet (Table 6).

Bottom Line: There are no effects of dietary oil or MOS in the skin mucosal patterns.Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut-associated immune system (GALT)-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth.However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Investigación en Acuicultura (GIA), Universidad de Las Palmas de Gran Canaria , Las Palmas de Gran Canaria , Spain.

ABSTRACT
The main objective of this study was to assess the effects of 4 g kg(-1) dietary mannan oligosaccharides (MOS) inclusion in soybean oil (SBO)- and fish oil (FO)-based diets on the gut health and skin mucosa mucus production of European sea bass juveniles after 8 weeks of feeding. Dietary MOS, regardless of the oil source, promoted growth. The intestinal somatic index was not affected, however dietary SBO reduced the intestinal fold length, while dietary MOS increased it. The dietary oil source fed produced changes on the posterior intestine fatty acid profiles irrespective of MOS dietary supplementation. SBO down-regulated the gene expression of TCRβ, COX2, IL-1β, TNFα, IL-8, IL-6, IL-10, TGFβ, and Ig and up-regulated MHCII. MOS supplementation up-regulated the expression of MHCI, CD4, COX2, TNFα, and Ig when included in FO-based diets. However, there was a minor up-regulating effect on these genes when MOS was supplemented in the SBO-based diet. Both dietary oil sources and MOS affected mean mucous cell areas within the posterior gut, however the addition of MOS to a SBO diet increased the mucous cell size over the values shown in FO fed fish. Dietary SBO also trends to reduce mucous cell density in the anterior gut relative to FO, suggesting a lower overall mucosal secretion. There are no effects of dietary oil or MOS in the skin mucosal patterns. Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut-associated immune system (GALT)-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth. However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis. This denotes the importance of a balanced dietary n-3/n-6 ratio for an appropriate GALT-immune response against MOS in European sea bass juveniles.

No MeSH data available.


Related in: MedlinePlus