Limits...
Porcine epidemic diarrhea: a review of current epidemiology and available vaccines.

Song D, Moon H, Kang B - Clin Exp Vaccine Res (2015)

Bottom Line: Although PEDV was first identified in Europe, it has resulted in significant economic losses in many Asian swine-raising countries, including Korea, China, Japan, Vietnam, and the Philippines.Moreover, intercontinental transmission of PEDV has increased mortality rates in seronegative neonatal piglets, resulting in 10% loss of the US pig population.We also discuss PEDV vaccines and related issues.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Korea.

ABSTRACT
Porcine epidemic diarrhea virus (PEDV), an Alphacoronavirus in the family Coronaviridae, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. PEDV can also cause diarrhea, agalactia, and abnormal reproductive cycles in pregnant sows. Although PEDV was first identified in Europe, it has resulted in significant economic losses in many Asian swine-raising countries, including Korea, China, Japan, Vietnam, and the Philippines. However, from April 2013 to the present, major outbreaks of PEDV have been reported in the United States, Canada, and Mexico. Moreover, intercontinental transmission of PEDV has increased mortality rates in seronegative neonatal piglets, resulting in 10% loss of the US pig population. The emergence and re-emergence of PEDV indicates that the virus is able to evade current vaccine strategies. Continuous emergence of multiple mutant strains from several regions has aggravated porcine epidemic diarrhea endemic conditions and highlighted the need for new vaccines based on the current circulating PEDV. Epidemic PEDV strains tend to be more pathogenic and cause increased death in pigs, thereby causing substantial financial losses for swine producers. In this review, we described the epidemiology of PEDV in several countries and present molecular characterization of current strains. We also discuss PEDV vaccines and related issues.

No MeSH data available.


Related in: MedlinePlus

The multiplex RT-PCR assay and dual priming oligonucleotide (DPO) system can be used in diagnosis of porcine epidemic diarrhea virus (PEDV). PEC, porcine enteric calici virus; TGEV, transmissible gastroenteritis virus; PoTV, porcine torovirus; RotaA, rotavirus type A.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4524901&req=5

Figure 2: The multiplex RT-PCR assay and dual priming oligonucleotide (DPO) system can be used in diagnosis of porcine epidemic diarrhea virus (PEDV). PEC, porcine enteric calici virus; TGEV, transmissible gastroenteritis virus; PoTV, porcine torovirus; RotaA, rotavirus type A.

Mentions: Several reports have described the development of RT-PCR as a diagnostic technique for detection of both laboratory and field isolates [56575859]. Primers derived from the M gene can be used in an RT-PCR system to obtain PEDV-specific fragments [57], and duplex RT-PCR has been used to differentiate between TGEV and PEDV [60]. Within the past few years, several useful modifications of the basic RT-PCR method have been reported. For example, it is possible to estimate the potential transmission of PEDV by comparing viral shedding load with a standard internal control DNA curve [61] and by multiplex RT-PCR to detect PEDV in the presence of various viruses [62]-a technique that is particularly useful for rapid, sensitive, and cost-effective diagnosis of acute viral gastroenteritis in swine. The commercial dual priming oligonucleotide system (Seegene, Seoul, Korea) (Fig. 2) has been developed for the rapid differential detection of PEDV. This system employs a single tube one-step multiplex RT-PCR with two separate primer segments to block nonspecific priming [63]. Recently, a protein-based enzyme-linked immunosorbent assay (ELISA) system was developed to detect PEDV. Using this technique, a polyclonal antibody is produced by immunizing rabbits with purified PEDV M gene after its expression in Escherichia coli. Immunofluorescence analysis can then be carried out with the anti-PEDV-M antibody in order to detect PEDV-infected cells among other enteric viruses [64].


Porcine epidemic diarrhea: a review of current epidemiology and available vaccines.

Song D, Moon H, Kang B - Clin Exp Vaccine Res (2015)

The multiplex RT-PCR assay and dual priming oligonucleotide (DPO) system can be used in diagnosis of porcine epidemic diarrhea virus (PEDV). PEC, porcine enteric calici virus; TGEV, transmissible gastroenteritis virus; PoTV, porcine torovirus; RotaA, rotavirus type A.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524901&req=5

Figure 2: The multiplex RT-PCR assay and dual priming oligonucleotide (DPO) system can be used in diagnosis of porcine epidemic diarrhea virus (PEDV). PEC, porcine enteric calici virus; TGEV, transmissible gastroenteritis virus; PoTV, porcine torovirus; RotaA, rotavirus type A.
Mentions: Several reports have described the development of RT-PCR as a diagnostic technique for detection of both laboratory and field isolates [56575859]. Primers derived from the M gene can be used in an RT-PCR system to obtain PEDV-specific fragments [57], and duplex RT-PCR has been used to differentiate between TGEV and PEDV [60]. Within the past few years, several useful modifications of the basic RT-PCR method have been reported. For example, it is possible to estimate the potential transmission of PEDV by comparing viral shedding load with a standard internal control DNA curve [61] and by multiplex RT-PCR to detect PEDV in the presence of various viruses [62]-a technique that is particularly useful for rapid, sensitive, and cost-effective diagnosis of acute viral gastroenteritis in swine. The commercial dual priming oligonucleotide system (Seegene, Seoul, Korea) (Fig. 2) has been developed for the rapid differential detection of PEDV. This system employs a single tube one-step multiplex RT-PCR with two separate primer segments to block nonspecific priming [63]. Recently, a protein-based enzyme-linked immunosorbent assay (ELISA) system was developed to detect PEDV. Using this technique, a polyclonal antibody is produced by immunizing rabbits with purified PEDV M gene after its expression in Escherichia coli. Immunofluorescence analysis can then be carried out with the anti-PEDV-M antibody in order to detect PEDV-infected cells among other enteric viruses [64].

Bottom Line: Although PEDV was first identified in Europe, it has resulted in significant economic losses in many Asian swine-raising countries, including Korea, China, Japan, Vietnam, and the Philippines.Moreover, intercontinental transmission of PEDV has increased mortality rates in seronegative neonatal piglets, resulting in 10% loss of the US pig population.We also discuss PEDV vaccines and related issues.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Korea.

ABSTRACT
Porcine epidemic diarrhea virus (PEDV), an Alphacoronavirus in the family Coronaviridae, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. PEDV can also cause diarrhea, agalactia, and abnormal reproductive cycles in pregnant sows. Although PEDV was first identified in Europe, it has resulted in significant economic losses in many Asian swine-raising countries, including Korea, China, Japan, Vietnam, and the Philippines. However, from April 2013 to the present, major outbreaks of PEDV have been reported in the United States, Canada, and Mexico. Moreover, intercontinental transmission of PEDV has increased mortality rates in seronegative neonatal piglets, resulting in 10% loss of the US pig population. The emergence and re-emergence of PEDV indicates that the virus is able to evade current vaccine strategies. Continuous emergence of multiple mutant strains from several regions has aggravated porcine epidemic diarrhea endemic conditions and highlighted the need for new vaccines based on the current circulating PEDV. Epidemic PEDV strains tend to be more pathogenic and cause increased death in pigs, thereby causing substantial financial losses for swine producers. In this review, we described the epidemiology of PEDV in several countries and present molecular characterization of current strains. We also discuss PEDV vaccines and related issues.

No MeSH data available.


Related in: MedlinePlus