Limits...
Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats.

Lee SH, Jin KS, Bang OY, Kim BJ, Park SJ, Lee NH, Yoo KH, Koo HH, Sung KW - PLoS ONE (2015)

Bottom Line: The pattern of MCP-1 and SDF-1 level changes according to the time after MCAo was consistent with in vivo and in vitro migration patterns of MSCs.The results suggest that an earlier MSC transplantation is associated with a better functional recovery after stroke, which could be explained by the preferential migration of MSCs to the cortex in the early transplantation group.The time-dependent differential expression of MCP-1 and SDF-1 between ischemic regions seemed to mediate the differential migration of MSCs.

View Article: PubMed Central - PubMed

Affiliation: Samsung Genome Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

ABSTRACT
To evaluate the optimal timing of mesenchymal stem cell (MSC) transplantation following stroke, rats were transplanted with MSCs at 1 (D1), 4 (D4), and 7 days (D7) after middle cerebral artery occlusion (MCAo). Rats in the D1 group showed a better functional recovery than those in the D4 or D7 groups after MCAo. MSCs preferentially migrated to the cortex in the D1 group, while the MSCs in the D4 or D7 groups preferentially migrated to the striatum. Interestingly, the level of monocyte chemotactic protein-1 (MCP-1) in the cortex was highest at 1 day after MCAo, while the level of stromal cell-derived factor-1 (SDF-1) in the striatum was lowest at 1 day after MCAo and then increased over time. The pattern of MCP-1 and SDF-1 level changes according to the time after MCAo was consistent with in vivo and in vitro migration patterns of MSCs. The results suggest that an earlier MSC transplantation is associated with a better functional recovery after stroke, which could be explained by the preferential migration of MSCs to the cortex in the early transplantation group. The time-dependent differential expression of MCP-1 and SDF-1 between ischemic regions seemed to mediate the differential migration of MSCs. Highest level of MCP-1 at one day of stroke may induce preferential migration of MSCs to the cortex, then better functional improvement.

No MeSH data available.


Related in: MedlinePlus

Modified Neurological Severity Score (mNSS) results according to the time after MCAo.The mNSS was lower in the D1 group compared to the control (PBS group), D4, or D7 groups at each time point 14 days after MCAo (*P < 0.05) (n = 7 per group). MCAo indicates middle cerebral artery occlusion.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4524688&req=5

pone.0134920.g002: Modified Neurological Severity Score (mNSS) results according to the time after MCAo.The mNSS was lower in the D1 group compared to the control (PBS group), D4, or D7 groups at each time point 14 days after MCAo (*P < 0.05) (n = 7 per group). MCAo indicates middle cerebral artery occlusion.

Mentions: No difference was observed in the mNSS evaluation between groups at day 0 and day 1 of MCAo. Prominent functional improvement was observed in the D1 group compared to the control (PBS group), D4, or D7 groups at each time point 14 days after MCAo (Fig 2). The D4 and D7 groups also showed a functional improvement trend compared to the control group; however, it was not statistically significant (n = 7 per group).


Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats.

Lee SH, Jin KS, Bang OY, Kim BJ, Park SJ, Lee NH, Yoo KH, Koo HH, Sung KW - PLoS ONE (2015)

Modified Neurological Severity Score (mNSS) results according to the time after MCAo.The mNSS was lower in the D1 group compared to the control (PBS group), D4, or D7 groups at each time point 14 days after MCAo (*P < 0.05) (n = 7 per group). MCAo indicates middle cerebral artery occlusion.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524688&req=5

pone.0134920.g002: Modified Neurological Severity Score (mNSS) results according to the time after MCAo.The mNSS was lower in the D1 group compared to the control (PBS group), D4, or D7 groups at each time point 14 days after MCAo (*P < 0.05) (n = 7 per group). MCAo indicates middle cerebral artery occlusion.
Mentions: No difference was observed in the mNSS evaluation between groups at day 0 and day 1 of MCAo. Prominent functional improvement was observed in the D1 group compared to the control (PBS group), D4, or D7 groups at each time point 14 days after MCAo (Fig 2). The D4 and D7 groups also showed a functional improvement trend compared to the control group; however, it was not statistically significant (n = 7 per group).

Bottom Line: The pattern of MCP-1 and SDF-1 level changes according to the time after MCAo was consistent with in vivo and in vitro migration patterns of MSCs.The results suggest that an earlier MSC transplantation is associated with a better functional recovery after stroke, which could be explained by the preferential migration of MSCs to the cortex in the early transplantation group.The time-dependent differential expression of MCP-1 and SDF-1 between ischemic regions seemed to mediate the differential migration of MSCs.

View Article: PubMed Central - PubMed

Affiliation: Samsung Genome Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

ABSTRACT
To evaluate the optimal timing of mesenchymal stem cell (MSC) transplantation following stroke, rats were transplanted with MSCs at 1 (D1), 4 (D4), and 7 days (D7) after middle cerebral artery occlusion (MCAo). Rats in the D1 group showed a better functional recovery than those in the D4 or D7 groups after MCAo. MSCs preferentially migrated to the cortex in the D1 group, while the MSCs in the D4 or D7 groups preferentially migrated to the striatum. Interestingly, the level of monocyte chemotactic protein-1 (MCP-1) in the cortex was highest at 1 day after MCAo, while the level of stromal cell-derived factor-1 (SDF-1) in the striatum was lowest at 1 day after MCAo and then increased over time. The pattern of MCP-1 and SDF-1 level changes according to the time after MCAo was consistent with in vivo and in vitro migration patterns of MSCs. The results suggest that an earlier MSC transplantation is associated with a better functional recovery after stroke, which could be explained by the preferential migration of MSCs to the cortex in the early transplantation group. The time-dependent differential expression of MCP-1 and SDF-1 between ischemic regions seemed to mediate the differential migration of MSCs. Highest level of MCP-1 at one day of stroke may induce preferential migration of MSCs to the cortex, then better functional improvement.

No MeSH data available.


Related in: MedlinePlus