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Identification of Candidate Coral Pathogens on White Band Disease-Infected Staghorn Coral.

Gignoux-Wolfsohn SA, Vollmer SV - PLoS ONE (2015)

Bottom Line: We also exposed corals in tanks to diseased and healthy (control) homogenates to reduce some of the natural variation of field-collected coral bacterial communities.A large percentage of these disease-associated OTUs belonged to the order Flavobacteriales.In addition, two of the putative pathogens identified here belong to orders previously suggested as WBD pathogens: Vibronales and Rickettsiales.

View Article: PubMed Central - PubMed

Affiliation: Marine Science Center, Northeastern University, Nahant, Massachusetts, United States of America.

ABSTRACT
Bacterial diseases affecting scleractinian corals pose an enormous threat to the health of coral reefs, yet we still have a limited understanding of the bacteria associated with coral diseases. White band disease is a bacterial disease that affects the two Caribbean acroporid corals, the staghorn coral Acropora cervicornis and the elkhorn coral A. palmate. Species of Vibrio and Rickettsia have both been identified as putative WBD pathogens. Here we used Illumina 16S rRNA gene sequencing to profile the bacterial communities associated with healthy and diseased A. cervicornis collected from four field sites during two different years. We also exposed corals in tanks to diseased and healthy (control) homogenates to reduce some of the natural variation of field-collected coral bacterial communities. Using a combination of multivariate analyses, we identified community-level changes between diseased and healthy corals in both the field-collected and tank-exposed datasets. We then identified changes in the abundances of individual operational taxonomic units (OTUs) between diseased and healthy corals. By comparing the diseased and healthy-associated bacteria in field-collected and tank-exposed corals, we were able to identify 16 healthy-associated OTUs and 106 consistently disease-associated OTUs, which are good candidates for putative WBD pathogens. A large percentage of these disease-associated OTUs belonged to the order Flavobacteriales. In addition, two of the putative pathogens identified here belong to orders previously suggested as WBD pathogens: Vibronales and Rickettsiales.

No MeSH data available.


Related in: MedlinePlus

Plots of the log2 fold abundance change of each OTU by the mean of normalized counts.Significantly more or less abundant OTUs are in red (a) Field-collected corals by year + disease state (b) Tank-exposed corals by final disease state.
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pone.0134416.g002: Plots of the log2 fold abundance change of each OTU by the mean of normalized counts.Significantly more or less abundant OTUs are in red (a) Field-collected corals by year + disease state (b) Tank-exposed corals by final disease state.

Mentions: The negative binomial GLM on the field data comparing the abundance of OTUs across disease state and year identified 1,363 individual OTUs that differed significantly due to disease state, 20 OTUs that differed significantly due to year, and 66 OTUs that differed significantly due to the interaction of disease state and year (Table 5). The majority of the OTUs that differed due to disease state (1,012 or 74%) were significantly more abundant on diseased corals than healthy corals (Fig 2A). In the tank-infected corals, the negative binomial GLM comparing disease-exposed corals that contracted disease (DD) with healthy-exposed (i.e. control) corals (HH) identified 521 OTUs associated with disease state, the majority of which (n = 494; 95%) were more abundant on the disease-infected corals (Fig 2B).


Identification of Candidate Coral Pathogens on White Band Disease-Infected Staghorn Coral.

Gignoux-Wolfsohn SA, Vollmer SV - PLoS ONE (2015)

Plots of the log2 fold abundance change of each OTU by the mean of normalized counts.Significantly more or less abundant OTUs are in red (a) Field-collected corals by year + disease state (b) Tank-exposed corals by final disease state.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524643&req=5

pone.0134416.g002: Plots of the log2 fold abundance change of each OTU by the mean of normalized counts.Significantly more or less abundant OTUs are in red (a) Field-collected corals by year + disease state (b) Tank-exposed corals by final disease state.
Mentions: The negative binomial GLM on the field data comparing the abundance of OTUs across disease state and year identified 1,363 individual OTUs that differed significantly due to disease state, 20 OTUs that differed significantly due to year, and 66 OTUs that differed significantly due to the interaction of disease state and year (Table 5). The majority of the OTUs that differed due to disease state (1,012 or 74%) were significantly more abundant on diseased corals than healthy corals (Fig 2A). In the tank-infected corals, the negative binomial GLM comparing disease-exposed corals that contracted disease (DD) with healthy-exposed (i.e. control) corals (HH) identified 521 OTUs associated with disease state, the majority of which (n = 494; 95%) were more abundant on the disease-infected corals (Fig 2B).

Bottom Line: We also exposed corals in tanks to diseased and healthy (control) homogenates to reduce some of the natural variation of field-collected coral bacterial communities.A large percentage of these disease-associated OTUs belonged to the order Flavobacteriales.In addition, two of the putative pathogens identified here belong to orders previously suggested as WBD pathogens: Vibronales and Rickettsiales.

View Article: PubMed Central - PubMed

Affiliation: Marine Science Center, Northeastern University, Nahant, Massachusetts, United States of America.

ABSTRACT
Bacterial diseases affecting scleractinian corals pose an enormous threat to the health of coral reefs, yet we still have a limited understanding of the bacteria associated with coral diseases. White band disease is a bacterial disease that affects the two Caribbean acroporid corals, the staghorn coral Acropora cervicornis and the elkhorn coral A. palmate. Species of Vibrio and Rickettsia have both been identified as putative WBD pathogens. Here we used Illumina 16S rRNA gene sequencing to profile the bacterial communities associated with healthy and diseased A. cervicornis collected from four field sites during two different years. We also exposed corals in tanks to diseased and healthy (control) homogenates to reduce some of the natural variation of field-collected coral bacterial communities. Using a combination of multivariate analyses, we identified community-level changes between diseased and healthy corals in both the field-collected and tank-exposed datasets. We then identified changes in the abundances of individual operational taxonomic units (OTUs) between diseased and healthy corals. By comparing the diseased and healthy-associated bacteria in field-collected and tank-exposed corals, we were able to identify 16 healthy-associated OTUs and 106 consistently disease-associated OTUs, which are good candidates for putative WBD pathogens. A large percentage of these disease-associated OTUs belonged to the order Flavobacteriales. In addition, two of the putative pathogens identified here belong to orders previously suggested as WBD pathogens: Vibronales and Rickettsiales.

No MeSH data available.


Related in: MedlinePlus