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SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.

Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z, Hu Z - PLoS ONE (2015)

Bottom Line: The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge.Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues.Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

ABSTRACT
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

No MeSH data available.


Related in: MedlinePlus

Salt stress tolerance of SlDEAD31-overexpressing plants.(A) Growth characteristics of WT and transgenic tomato plants at 0, 14 and 21 d after salt stress. 8-week-old plants were irrigated with 200 mL 400 mM NaCl every 72 h from the bottom of the pots. Representative plants are shown. (B) Survival rate of the plants shown after 21 d of salt treatment and 5 d of recovery. Each bar represents an average of eight plants ± SE (n = 3). Asterisks indicate a significant difference between WT and transgenic lines (P<0.05). (C)-(E) Comparisons of chlorophyll content (C), relative water content (RWC, D), and malondialdehyde (MDA) content (E) of WT and transgenic plants at 0, 14 and 21 d after salt stress. Data represent the means ± SE (n = 3).
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pone.0133849.g006: Salt stress tolerance of SlDEAD31-overexpressing plants.(A) Growth characteristics of WT and transgenic tomato plants at 0, 14 and 21 d after salt stress. 8-week-old plants were irrigated with 200 mL 400 mM NaCl every 72 h from the bottom of the pots. Representative plants are shown. (B) Survival rate of the plants shown after 21 d of salt treatment and 5 d of recovery. Each bar represents an average of eight plants ± SE (n = 3). Asterisks indicate a significant difference between WT and transgenic lines (P<0.05). (C)-(E) Comparisons of chlorophyll content (C), relative water content (RWC, D), and malondialdehyde (MDA) content (E) of WT and transgenic plants at 0, 14 and 21 d after salt stress. Data represent the means ± SE (n = 3).

Mentions: To further evaluate the performance of SlDEAD31-overexpressing plants under salt stress in soil, 8-week-old plants of WT and transgenic lines were irrigated with water containing 400 mM NaCl (200 mL) every 72 h. Under normal growth conditions, the transgenic plants showed no abnormal morphological phenotype compared with WT plants (Fig 6A). However, WT plants showed more notorious and quick damage signs during all the time course of the salt assay. NaCl-induced symptoms were observable 14 d after salt stress with chlorosis and wilting of lower leaves in both WT and transgenic plants, whereas the transgenic lines displayed apparently less wilting and necrosis, and most of the leaves remained vigorous. After 21 d, all the leaves of WT plants showed severe necrosis and wilting, and some were dead, whereas the upper leaves of transgenic plants remained green and vigorous (Fig 6A). Besides, most of the WT plants were dead 5 d after stopping the treatment, whereas 43–62% of the transgenic plants survived (Fig 6B).


SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.

Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z, Hu Z - PLoS ONE (2015)

Salt stress tolerance of SlDEAD31-overexpressing plants.(A) Growth characteristics of WT and transgenic tomato plants at 0, 14 and 21 d after salt stress. 8-week-old plants were irrigated with 200 mL 400 mM NaCl every 72 h from the bottom of the pots. Representative plants are shown. (B) Survival rate of the plants shown after 21 d of salt treatment and 5 d of recovery. Each bar represents an average of eight plants ± SE (n = 3). Asterisks indicate a significant difference between WT and transgenic lines (P<0.05). (C)-(E) Comparisons of chlorophyll content (C), relative water content (RWC, D), and malondialdehyde (MDA) content (E) of WT and transgenic plants at 0, 14 and 21 d after salt stress. Data represent the means ± SE (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524616&req=5

pone.0133849.g006: Salt stress tolerance of SlDEAD31-overexpressing plants.(A) Growth characteristics of WT and transgenic tomato plants at 0, 14 and 21 d after salt stress. 8-week-old plants were irrigated with 200 mL 400 mM NaCl every 72 h from the bottom of the pots. Representative plants are shown. (B) Survival rate of the plants shown after 21 d of salt treatment and 5 d of recovery. Each bar represents an average of eight plants ± SE (n = 3). Asterisks indicate a significant difference between WT and transgenic lines (P<0.05). (C)-(E) Comparisons of chlorophyll content (C), relative water content (RWC, D), and malondialdehyde (MDA) content (E) of WT and transgenic plants at 0, 14 and 21 d after salt stress. Data represent the means ± SE (n = 3).
Mentions: To further evaluate the performance of SlDEAD31-overexpressing plants under salt stress in soil, 8-week-old plants of WT and transgenic lines were irrigated with water containing 400 mM NaCl (200 mL) every 72 h. Under normal growth conditions, the transgenic plants showed no abnormal morphological phenotype compared with WT plants (Fig 6A). However, WT plants showed more notorious and quick damage signs during all the time course of the salt assay. NaCl-induced symptoms were observable 14 d after salt stress with chlorosis and wilting of lower leaves in both WT and transgenic plants, whereas the transgenic lines displayed apparently less wilting and necrosis, and most of the leaves remained vigorous. After 21 d, all the leaves of WT plants showed severe necrosis and wilting, and some were dead, whereas the upper leaves of transgenic plants remained green and vigorous (Fig 6A). Besides, most of the WT plants were dead 5 d after stopping the treatment, whereas 43–62% of the transgenic plants survived (Fig 6B).

Bottom Line: The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge.Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues.Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

ABSTRACT
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

No MeSH data available.


Related in: MedlinePlus