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SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.

Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z, Hu Z - PLoS ONE (2015)

Bottom Line: The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge.Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues.Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

ABSTRACT
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

No MeSH data available.


Related in: MedlinePlus

Expression patterns of SlDEAD30 and SlDEAD31 genes under various stress treatments including NaCl (200 mM, A, D), heat (40°C) and cold (4°C, B, E), dehydration, and wounding (C, F).Gene expression was detected by RT-PCR using total RNA from leaves or roots of 35-day-old tomato plants. Bars represent mean relative expression values ± SE (n = 3). Values are presented relative to untreated plants (0 h). Asterisks indicate a significant difference (P<0.05) between untreated and treated plants. The twofold threshold is indicated by a dotted line.
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pone.0133849.g004: Expression patterns of SlDEAD30 and SlDEAD31 genes under various stress treatments including NaCl (200 mM, A, D), heat (40°C) and cold (4°C, B, E), dehydration, and wounding (C, F).Gene expression was detected by RT-PCR using total RNA from leaves or roots of 35-day-old tomato plants. Bars represent mean relative expression values ± SE (n = 3). Values are presented relative to untreated plants (0 h). Asterisks indicate a significant difference (P<0.05) between untreated and treated plants. The twofold threshold is indicated by a dotted line.

Mentions: To speculate on the physiological and functional relevance of SlDEAD30 and SlDEAD31 genes, we detected their expression profiles under various abiotic stresses by quantitative RT-PCR. We found that their expression was not affected by circadian rhythm under the normal growth condition (S3 Fig). SlDEAD30 transcripts were induced apparently in roots after NaCl stress compared with controls, and to a lesser degree in leaves (Fig 4A). SlDEAD31 expression was also induced by NaCl in roots, whereas remained unchanged in leaves (Fig 4D). The results indicate that the expression of both SlDEAD30 and SlDEAD31 was induced mainly in roots but not in leaves under NaCl stress. When treated with heat and cold, SlDEAD30 mRNA was not increased by cold, while down-regulated by heat (Fig 4B). In contrast, SlDEAD31 expression was induced by both stresses (Fig 4E). In addition, the expression of SlDEAD30 was not induced by dehydration and wounding, while SlDEAD31 mRNA was induced by dehydration almost by a gradual increase (Fig 4C and 4F). The induction of SlDEAD30 and SlDEAD31 genes by abiotic stress prompted us to check their promoter sequences by searching against the promoter database PLACE. The results show that their promoter sequences indeed contain putative stress response-related cis-elements, such as MYB, MYC, and WRKY recognition sites (S2 Table).


SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.

Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z, Hu Z - PLoS ONE (2015)

Expression patterns of SlDEAD30 and SlDEAD31 genes under various stress treatments including NaCl (200 mM, A, D), heat (40°C) and cold (4°C, B, E), dehydration, and wounding (C, F).Gene expression was detected by RT-PCR using total RNA from leaves or roots of 35-day-old tomato plants. Bars represent mean relative expression values ± SE (n = 3). Values are presented relative to untreated plants (0 h). Asterisks indicate a significant difference (P<0.05) between untreated and treated plants. The twofold threshold is indicated by a dotted line.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524616&req=5

pone.0133849.g004: Expression patterns of SlDEAD30 and SlDEAD31 genes under various stress treatments including NaCl (200 mM, A, D), heat (40°C) and cold (4°C, B, E), dehydration, and wounding (C, F).Gene expression was detected by RT-PCR using total RNA from leaves or roots of 35-day-old tomato plants. Bars represent mean relative expression values ± SE (n = 3). Values are presented relative to untreated plants (0 h). Asterisks indicate a significant difference (P<0.05) between untreated and treated plants. The twofold threshold is indicated by a dotted line.
Mentions: To speculate on the physiological and functional relevance of SlDEAD30 and SlDEAD31 genes, we detected their expression profiles under various abiotic stresses by quantitative RT-PCR. We found that their expression was not affected by circadian rhythm under the normal growth condition (S3 Fig). SlDEAD30 transcripts were induced apparently in roots after NaCl stress compared with controls, and to a lesser degree in leaves (Fig 4A). SlDEAD31 expression was also induced by NaCl in roots, whereas remained unchanged in leaves (Fig 4D). The results indicate that the expression of both SlDEAD30 and SlDEAD31 was induced mainly in roots but not in leaves under NaCl stress. When treated with heat and cold, SlDEAD30 mRNA was not increased by cold, while down-regulated by heat (Fig 4B). In contrast, SlDEAD31 expression was induced by both stresses (Fig 4E). In addition, the expression of SlDEAD30 was not induced by dehydration and wounding, while SlDEAD31 mRNA was induced by dehydration almost by a gradual increase (Fig 4C and 4F). The induction of SlDEAD30 and SlDEAD31 genes by abiotic stress prompted us to check their promoter sequences by searching against the promoter database PLACE. The results show that their promoter sequences indeed contain putative stress response-related cis-elements, such as MYB, MYC, and WRKY recognition sites (S2 Table).

Bottom Line: The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge.Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues.Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

ABSTRACT
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

No MeSH data available.


Related in: MedlinePlus