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SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.

Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z, Hu Z - PLoS ONE (2015)

Bottom Line: Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues.In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants.Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

ABSTRACT
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of SlDEAD30 and SlDEAD31 genes in different tissues of wild-type tomato.Ro, roots; St, stems; Yl, young leaves; Ml, mature leaves; Sl, senescent leaves; Fl, flowers; Se, sepals; IMG, immature green fruits; MG, mature green fruits; B, breaker fruits; B+4, 4 day after breaker stage; B+7, 7 day after breaker stage. The relative expression levels were normalized to 1 in the roots. Bars represent mean relative expression values ± SE (n = 3).
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pone.0133849.g003: Expression profiles of SlDEAD30 and SlDEAD31 genes in different tissues of wild-type tomato.Ro, roots; St, stems; Yl, young leaves; Ml, mature leaves; Sl, senescent leaves; Fl, flowers; Se, sepals; IMG, immature green fruits; MG, mature green fruits; B, breaker fruits; B+4, 4 day after breaker stage; B+7, 7 day after breaker stage. The relative expression levels were normalized to 1 in the roots. Bars represent mean relative expression values ± SE (n = 3).

Mentions: Tissue specificity may be associated with specific biological functions. Previous reports have demonstrated that a range of DEAD-box RNA helicases are involved in regulating the essential steps in plant growth and development, such as morphogenesis and embryogenesis [18, 26], floral meristems [27], and chloroplast development [28]. The results showed that the expression of SlDEAD30 was relatively higher in roots and mature leaves, whereas moderate to weak signals of its expression were detected in other tissues such as stems, flowers, and fruits. SlDEAD31 mRNAs were constantly expressed in all the organs tested (Fig 3).


SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.

Zhu M, Chen G, Dong T, Wang L, Zhang J, Zhao Z, Hu Z - PLoS ONE (2015)

Expression profiles of SlDEAD30 and SlDEAD31 genes in different tissues of wild-type tomato.Ro, roots; St, stems; Yl, young leaves; Ml, mature leaves; Sl, senescent leaves; Fl, flowers; Se, sepals; IMG, immature green fruits; MG, mature green fruits; B, breaker fruits; B+4, 4 day after breaker stage; B+7, 7 day after breaker stage. The relative expression levels were normalized to 1 in the roots. Bars represent mean relative expression values ± SE (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524616&req=5

pone.0133849.g003: Expression profiles of SlDEAD30 and SlDEAD31 genes in different tissues of wild-type tomato.Ro, roots; St, stems; Yl, young leaves; Ml, mature leaves; Sl, senescent leaves; Fl, flowers; Se, sepals; IMG, immature green fruits; MG, mature green fruits; B, breaker fruits; B+4, 4 day after breaker stage; B+7, 7 day after breaker stage. The relative expression levels were normalized to 1 in the roots. Bars represent mean relative expression values ± SE (n = 3).
Mentions: Tissue specificity may be associated with specific biological functions. Previous reports have demonstrated that a range of DEAD-box RNA helicases are involved in regulating the essential steps in plant growth and development, such as morphogenesis and embryogenesis [18, 26], floral meristems [27], and chloroplast development [28]. The results showed that the expression of SlDEAD30 was relatively higher in roots and mature leaves, whereas moderate to weak signals of its expression were detected in other tissues such as stems, flowers, and fruits. SlDEAD31 mRNAs were constantly expressed in all the organs tested (Fig 3).

Bottom Line: Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues.In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants.Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

ABSTRACT
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

No MeSH data available.


Related in: MedlinePlus