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Finasteride Has Regionally Different Effects on Brain Oxidative Stress and Acetylcholinesterase Activity in Acute Thioacetamide-Induced Hepatic Encephalopathy in Rats.

Mladenović D, Petronijević N, Stojković T, Velimirović M, Jevtić G, Hrnčić D, Radosavljević T, Rašić-Marković A, Maksić N, Djuric D, Stanojlović O - PLoS ONE (2015)

Bottom Line: FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity.Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs.TAA group (p<0.01).

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailovic", Faculty of Medicine, University of Belgrade, Dr Subotica 9, Belgrade, Serbia.

ABSTRACT
Finasteride (FIN) inhibits neurosteroid synthesis and potentially improves the course of hepatic encephalopathy (HE). This study aimed to investigate the effects of FIN on brain oxidative stress and acetylcholinesterase (AchE) activity in acute thioacetamide-induced HE in rats. Male Wistar rats were divided into groups: 1. control; 2. thioacetamide-treated group (TAA; 900 mg/kg); 3. finasteride-treated group (FIN; 150 mg/kg); 4. group treated with FIN and TAA (FIN+TAA). Daily doses of FIN (50 mg/kg) and TAA (300 mg/kg) were administered intraperitoneally during three days and in FIN+TAA group FIN was administered 2h before every dose of TAA. FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity. Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs. TAA group (p<0.05). GPx activity was lower in caudate nucleus in FIN+TAA vs. TAA group (p<0.01). FIN pretreatment prevented TAA-induced rise in AchE activity in the thalamus and caudate nucleus and AchE activity correlates inversely in the thalamus (p<0.05) and positively in caudate nucleus (p<0.01) with malondialdehyde level. FIN has regionally selective effects on oxidative stress and AchE activity in the brain in acute TAA-induced HE in rats. The prooxidant role of FIN in the thalamus may be causally linked with inhibition of AchE.

No MeSH data available.


Related in: MedlinePlus

The effects of finasteride (FIN) and thioacetamide (TAA) on (a) reduced glutathione (GSH) level, (b) glutathione peroxidase (GPx), (c) glutathione reductase (GR) and (d) catalase activity in various brain regions.The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, #p<0.05 and ##p<0.01 vs. TAA group in the same region). For details see caption for Figs 1 and 2.
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pone.0134434.g004: The effects of finasteride (FIN) and thioacetamide (TAA) on (a) reduced glutathione (GSH) level, (b) glutathione peroxidase (GPx), (c) glutathione reductase (GR) and (d) catalase activity in various brain regions.The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, #p<0.05 and ##p<0.01 vs. TAA group in the same region). For details see caption for Figs 1 and 2.

Mentions: GSH level in TAA group was significantly higher in the cortex (104.29 ± 31.24 nmol/mg prot., p<0.01) and significantly lower in the hippocampus (24.05 ± 7.92 nmol/mg prot., p<0.05) and caudate nucleus (23.46 ± 3.71 nmol/mg prot., p<0.05) when compared with control. Similar to TAA group, in FIN+TAA group GSH level was significantly higher in the cortex (86.52 ± 27.23 nmol/mg prot., p<0.01) and significantly lower in caudate nucleus (13.18 ± 2.79 nmol/mg prot., p<0.01) by comparison with control group (43.98 ± 14.04 nmol/mg prot. and 34.70 ± 9.91 nmol/mg prot. in the cortex and caudate nucleus respectively). However, in caudate nucleus GSH level was significantly lower in FIN+TAA vs. TAA group (p<0.01), while in the cortex no significant difference in GSH level was evident between these experimental groups (p>0.05). Hippocampal GSH level was not significantly different in FIN+TAA (34.26 ± 9.17 nmol/mg prot.) vs. control group (39.67 ± 7.11 nmol/mg prot., p>0.05). Additionally, while TAA alone did not induce significant changes in thalamic GSH level (p>0.05), its level in the thalamus was significantly lower in FIN+TAA group (19.37 ± 3.31 nmol/mg prot., p<0.05) by comparison with control (28.76 ± 7.37 nmol/mg prot., Fig 4A).


Finasteride Has Regionally Different Effects on Brain Oxidative Stress and Acetylcholinesterase Activity in Acute Thioacetamide-Induced Hepatic Encephalopathy in Rats.

Mladenović D, Petronijević N, Stojković T, Velimirović M, Jevtić G, Hrnčić D, Radosavljević T, Rašić-Marković A, Maksić N, Djuric D, Stanojlović O - PLoS ONE (2015)

The effects of finasteride (FIN) and thioacetamide (TAA) on (a) reduced glutathione (GSH) level, (b) glutathione peroxidase (GPx), (c) glutathione reductase (GR) and (d) catalase activity in various brain regions.The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, #p<0.05 and ##p<0.01 vs. TAA group in the same region). For details see caption for Figs 1 and 2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524603&req=5

pone.0134434.g004: The effects of finasteride (FIN) and thioacetamide (TAA) on (a) reduced glutathione (GSH) level, (b) glutathione peroxidase (GPx), (c) glutathione reductase (GR) and (d) catalase activity in various brain regions.The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, #p<0.05 and ##p<0.01 vs. TAA group in the same region). For details see caption for Figs 1 and 2.
Mentions: GSH level in TAA group was significantly higher in the cortex (104.29 ± 31.24 nmol/mg prot., p<0.01) and significantly lower in the hippocampus (24.05 ± 7.92 nmol/mg prot., p<0.05) and caudate nucleus (23.46 ± 3.71 nmol/mg prot., p<0.05) when compared with control. Similar to TAA group, in FIN+TAA group GSH level was significantly higher in the cortex (86.52 ± 27.23 nmol/mg prot., p<0.01) and significantly lower in caudate nucleus (13.18 ± 2.79 nmol/mg prot., p<0.01) by comparison with control group (43.98 ± 14.04 nmol/mg prot. and 34.70 ± 9.91 nmol/mg prot. in the cortex and caudate nucleus respectively). However, in caudate nucleus GSH level was significantly lower in FIN+TAA vs. TAA group (p<0.01), while in the cortex no significant difference in GSH level was evident between these experimental groups (p>0.05). Hippocampal GSH level was not significantly different in FIN+TAA (34.26 ± 9.17 nmol/mg prot.) vs. control group (39.67 ± 7.11 nmol/mg prot., p>0.05). Additionally, while TAA alone did not induce significant changes in thalamic GSH level (p>0.05), its level in the thalamus was significantly lower in FIN+TAA group (19.37 ± 3.31 nmol/mg prot., p<0.05) by comparison with control (28.76 ± 7.37 nmol/mg prot., Fig 4A).

Bottom Line: FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity.Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs.TAA group (p<0.01).

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailovic", Faculty of Medicine, University of Belgrade, Dr Subotica 9, Belgrade, Serbia.

ABSTRACT
Finasteride (FIN) inhibits neurosteroid synthesis and potentially improves the course of hepatic encephalopathy (HE). This study aimed to investigate the effects of FIN on brain oxidative stress and acetylcholinesterase (AchE) activity in acute thioacetamide-induced HE in rats. Male Wistar rats were divided into groups: 1. control; 2. thioacetamide-treated group (TAA; 900 mg/kg); 3. finasteride-treated group (FIN; 150 mg/kg); 4. group treated with FIN and TAA (FIN+TAA). Daily doses of FIN (50 mg/kg) and TAA (300 mg/kg) were administered intraperitoneally during three days and in FIN+TAA group FIN was administered 2h before every dose of TAA. FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity. Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs. TAA group (p<0.05). GPx activity was lower in caudate nucleus in FIN+TAA vs. TAA group (p<0.01). FIN pretreatment prevented TAA-induced rise in AchE activity in the thalamus and caudate nucleus and AchE activity correlates inversely in the thalamus (p<0.05) and positively in caudate nucleus (p<0.01) with malondialdehyde level. FIN has regionally selective effects on oxidative stress and AchE activity in the brain in acute TAA-induced HE in rats. The prooxidant role of FIN in the thalamus may be causally linked with inhibition of AchE.

No MeSH data available.


Related in: MedlinePlus