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Finasteride Has Regionally Different Effects on Brain Oxidative Stress and Acetylcholinesterase Activity in Acute Thioacetamide-Induced Hepatic Encephalopathy in Rats.

Mladenović D, Petronijević N, Stojković T, Velimirović M, Jevtić G, Hrnčić D, Radosavljević T, Rašić-Marković A, Maksić N, Djuric D, Stanojlović O - PLoS ONE (2015)

Bottom Line: FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity.Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs.TAA group (p<0.01).

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailovic", Faculty of Medicine, University of Belgrade, Dr Subotica 9, Belgrade, Serbia.

ABSTRACT
Finasteride (FIN) inhibits neurosteroid synthesis and potentially improves the course of hepatic encephalopathy (HE). This study aimed to investigate the effects of FIN on brain oxidative stress and acetylcholinesterase (AchE) activity in acute thioacetamide-induced HE in rats. Male Wistar rats were divided into groups: 1. control; 2. thioacetamide-treated group (TAA; 900 mg/kg); 3. finasteride-treated group (FIN; 150 mg/kg); 4. group treated with FIN and TAA (FIN+TAA). Daily doses of FIN (50 mg/kg) and TAA (300 mg/kg) were administered intraperitoneally during three days and in FIN+TAA group FIN was administered 2h before every dose of TAA. FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity. Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs. TAA group (p<0.05). GPx activity was lower in caudate nucleus in FIN+TAA vs. TAA group (p<0.01). FIN pretreatment prevented TAA-induced rise in AchE activity in the thalamus and caudate nucleus and AchE activity correlates inversely in the thalamus (p<0.05) and positively in caudate nucleus (p<0.01) with malondialdehyde level. FIN has regionally selective effects on oxidative stress and AchE activity in the brain in acute TAA-induced HE in rats. The prooxidant role of FIN in the thalamus may be causally linked with inhibition of AchE.

No MeSH data available.


Related in: MedlinePlus

The effects of finasteride (FIN) and thioacetamide (TAA) on expression of cytosolic (SOD1) and mitochondrial SOD izoenzyme (SOD2) in the (a) cortex and (b) the hippocampus.For determination of expression polyclonal goat anti-SOD1 (1:500) and polyclonal goat anti-SOD2 (1:500) were used. After incubation with primary antibodies the membranes were incubated with the horseradish peroxidase (HRP) labeled secondary anti-goat antibody (1:2000) in TBST for 1 hour at room temperature. Five subsequent washes with 0.1% TBST were performed between each step. All membranes were stripped and re-probed with mouse monoclonal anti-actin antibodies (1:10000) to ensure that all wells were equally loaded. The signal was detected by enhanced chemiluminescence and subsequent exposure on an X-ray film. The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, ##p<0.01 vs. TAA group, †p<0.01 vs. FIN group). For details see caption for Fig 1.
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pone.0134434.g003: The effects of finasteride (FIN) and thioacetamide (TAA) on expression of cytosolic (SOD1) and mitochondrial SOD izoenzyme (SOD2) in the (a) cortex and (b) the hippocampus.For determination of expression polyclonal goat anti-SOD1 (1:500) and polyclonal goat anti-SOD2 (1:500) were used. After incubation with primary antibodies the membranes were incubated with the horseradish peroxidase (HRP) labeled secondary anti-goat antibody (1:2000) in TBST for 1 hour at room temperature. Five subsequent washes with 0.1% TBST were performed between each step. All membranes were stripped and re-probed with mouse monoclonal anti-actin antibodies (1:10000) to ensure that all wells were equally loaded. The signal was detected by enhanced chemiluminescence and subsequent exposure on an X-ray film. The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, ##p<0.01 vs. TAA group, †p<0.01 vs. FIN group). For details see caption for Fig 1.

Mentions: Analysis of SOD izoenzymes revealed that SOD1 expression in the cortex was significantly higher in all experimental groups vs. control. However, in FIN+TAA group the expression of this izoenzyme was significantly higher by comparison with TAA (p<0.01) and significantly lower when compared with FIN group (p<0.01, Fig 3A). In hippocampus SOD1 expression was significantly higher in FIN (p<0.05) and FIN+TAA group (p<0.05) by comparison with control, but no change in the expression of this izoenzyme in the hippocampus was evident in TAA vs. control group (p>0.05, Fig 3B). No significant changes in SOD2 expression among groups were evident in both brain regions.


Finasteride Has Regionally Different Effects on Brain Oxidative Stress and Acetylcholinesterase Activity in Acute Thioacetamide-Induced Hepatic Encephalopathy in Rats.

Mladenović D, Petronijević N, Stojković T, Velimirović M, Jevtić G, Hrnčić D, Radosavljević T, Rašić-Marković A, Maksić N, Djuric D, Stanojlović O - PLoS ONE (2015)

The effects of finasteride (FIN) and thioacetamide (TAA) on expression of cytosolic (SOD1) and mitochondrial SOD izoenzyme (SOD2) in the (a) cortex and (b) the hippocampus.For determination of expression polyclonal goat anti-SOD1 (1:500) and polyclonal goat anti-SOD2 (1:500) were used. After incubation with primary antibodies the membranes were incubated with the horseradish peroxidase (HRP) labeled secondary anti-goat antibody (1:2000) in TBST for 1 hour at room temperature. Five subsequent washes with 0.1% TBST were performed between each step. All membranes were stripped and re-probed with mouse monoclonal anti-actin antibodies (1:10000) to ensure that all wells were equally loaded. The signal was detected by enhanced chemiluminescence and subsequent exposure on an X-ray film. The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, ##p<0.01 vs. TAA group, †p<0.01 vs. FIN group). For details see caption for Fig 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524603&req=5

pone.0134434.g003: The effects of finasteride (FIN) and thioacetamide (TAA) on expression of cytosolic (SOD1) and mitochondrial SOD izoenzyme (SOD2) in the (a) cortex and (b) the hippocampus.For determination of expression polyclonal goat anti-SOD1 (1:500) and polyclonal goat anti-SOD2 (1:500) were used. After incubation with primary antibodies the membranes were incubated with the horseradish peroxidase (HRP) labeled secondary anti-goat antibody (1:2000) in TBST for 1 hour at room temperature. Five subsequent washes with 0.1% TBST were performed between each step. All membranes were stripped and re-probed with mouse monoclonal anti-actin antibodies (1:10000) to ensure that all wells were equally loaded. The signal was detected by enhanced chemiluminescence and subsequent exposure on an X-ray film. The significance of the difference was estimated by ANOVA with Fisher’s post hoc test (*p<0.05 and **p<0.01 vs. control, ##p<0.01 vs. TAA group, †p<0.01 vs. FIN group). For details see caption for Fig 1.
Mentions: Analysis of SOD izoenzymes revealed that SOD1 expression in the cortex was significantly higher in all experimental groups vs. control. However, in FIN+TAA group the expression of this izoenzyme was significantly higher by comparison with TAA (p<0.01) and significantly lower when compared with FIN group (p<0.01, Fig 3A). In hippocampus SOD1 expression was significantly higher in FIN (p<0.05) and FIN+TAA group (p<0.05) by comparison with control, but no change in the expression of this izoenzyme in the hippocampus was evident in TAA vs. control group (p>0.05, Fig 3B). No significant changes in SOD2 expression among groups were evident in both brain regions.

Bottom Line: FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity.Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs.TAA group (p<0.01).

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailovic", Faculty of Medicine, University of Belgrade, Dr Subotica 9, Belgrade, Serbia.

ABSTRACT
Finasteride (FIN) inhibits neurosteroid synthesis and potentially improves the course of hepatic encephalopathy (HE). This study aimed to investigate the effects of FIN on brain oxidative stress and acetylcholinesterase (AchE) activity in acute thioacetamide-induced HE in rats. Male Wistar rats were divided into groups: 1. control; 2. thioacetamide-treated group (TAA; 900 mg/kg); 3. finasteride-treated group (FIN; 150 mg/kg); 4. group treated with FIN and TAA (FIN+TAA). Daily doses of FIN (50 mg/kg) and TAA (300 mg/kg) were administered intraperitoneally during three days and in FIN+TAA group FIN was administered 2h before every dose of TAA. FIN pretreatment prevented TAA-induced rise in malondialdehyde level in the cortex due to restoration of catalase activity and increased expression of superoxide dismutase 1 (SOD1) and induced an increase in malondialdehyde level in the thalamus due to reduction of glutathione peroxidase (GPx) and glutathione reductase (GR) activity. Although FIN pretreatment did not affect malondialdehyde level in hippocampus and caudate nucleus, hippocampal SOD1 expression was higher (p<0.05) and GR activity lower in FIN+TAA vs. TAA group (p<0.05). GPx activity was lower in caudate nucleus in FIN+TAA vs. TAA group (p<0.01). FIN pretreatment prevented TAA-induced rise in AchE activity in the thalamus and caudate nucleus and AchE activity correlates inversely in the thalamus (p<0.05) and positively in caudate nucleus (p<0.01) with malondialdehyde level. FIN has regionally selective effects on oxidative stress and AchE activity in the brain in acute TAA-induced HE in rats. The prooxidant role of FIN in the thalamus may be causally linked with inhibition of AchE.

No MeSH data available.


Related in: MedlinePlus