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Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish.

Wu J, Xiong S, Jing J, Chen X, Wang W, Gui JF, Mei J - PLoS ONE (2015)

Bottom Line: In our previous study, significant differences in morphology and miRNA expression were detected between XY and YY testis of yellow catfish.Here, solexa sequencing technology was further performed to compare mRNA expression between XY and YY testis.KEGG analysis suggested different levels of PI3K-AKT and G protein-coupled receptor (GPCR) signaling pathways between XY and YY testis.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
YY super-males have rarely been detected in nature and only been artificially created in some fish species including tilapia and yellow catfish (Pelteobagrusfulvidraco), which provides a promising model for testis development and spermatogenesis. In our previous study, significant differences in morphology and miRNA expression were detected between XY and YY testis of yellow catfish. Here, solexa sequencing technology was further performed to compare mRNA expression between XY and YY testis. Compared with unigenes expressed in XY testis, 1146 and 1235 unigenes have significantly higher and lower expression in YY testis, respectively. 605 differentially expressed unigenes were annotated to 1604 GO terms with 319 and 286 genes having relative higher expression in XY and YY testis. KEGG analysis suggested different levels of PI3K-AKT and G protein-coupled receptor (GPCR) signaling pathways between XY and YY testis. Down-regulation of miR-141/429 in YY testis was speculated to promote testis development and maturation, and several factors in PI3K-AKT and GPCR signaling pathways were found as predicted targets of miR-141/429, several of which were confirmed by dual-luciferase reporter assays. Our study provides a comparative transcriptome analysis between XY and YY testis, and reveals interactions between miRNAs and their target genes that are possibly involved in regulating testis development and spermatogenesis.

No MeSH data available.


Related in: MedlinePlus

Species distribution of the top BlastX hits using the assembled unigenes of yellow catfish.The unigenes were searched against the NCBI Nr database with a cut-off E-value<1e-5.
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pone.0134626.g001: Species distribution of the top BlastX hits using the assembled unigenes of yellow catfish.The unigenes were searched against the NCBI Nr database with a cut-off E-value<1e-5.

Mentions: The unigenes of de novo assembly were searched against the NCBI non-redundant (nr), SWISS-PROT, KEGG, GO and KOG protein databases by using BLASTX with a cut-off E-value of 1e-5 (S1 Table). Finally, a total of 19,795 (25.33%) unigenes were significantly matched with nr database. Among these BlastX-hit unigenes (Fig 1), 11591 (58.56%) hits were assigned to Daniorerio while only 776 (3.92%) hits overlapped with Ictalurus punctatus (the close species to Pelteobagrus fulvidraco), perhaps due to the limited amount of genomic data on GeneBank about the species of Siluriformes. In addition, there were a number of unigenes without hitting results, which may contain non-coding fragments or some undetected genes.


Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish.

Wu J, Xiong S, Jing J, Chen X, Wang W, Gui JF, Mei J - PLoS ONE (2015)

Species distribution of the top BlastX hits using the assembled unigenes of yellow catfish.The unigenes were searched against the NCBI Nr database with a cut-off E-value<1e-5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524600&req=5

pone.0134626.g001: Species distribution of the top BlastX hits using the assembled unigenes of yellow catfish.The unigenes were searched against the NCBI Nr database with a cut-off E-value<1e-5.
Mentions: The unigenes of de novo assembly were searched against the NCBI non-redundant (nr), SWISS-PROT, KEGG, GO and KOG protein databases by using BLASTX with a cut-off E-value of 1e-5 (S1 Table). Finally, a total of 19,795 (25.33%) unigenes were significantly matched with nr database. Among these BlastX-hit unigenes (Fig 1), 11591 (58.56%) hits were assigned to Daniorerio while only 776 (3.92%) hits overlapped with Ictalurus punctatus (the close species to Pelteobagrus fulvidraco), perhaps due to the limited amount of genomic data on GeneBank about the species of Siluriformes. In addition, there were a number of unigenes without hitting results, which may contain non-coding fragments or some undetected genes.

Bottom Line: In our previous study, significant differences in morphology and miRNA expression were detected between XY and YY testis of yellow catfish.Here, solexa sequencing technology was further performed to compare mRNA expression between XY and YY testis.KEGG analysis suggested different levels of PI3K-AKT and G protein-coupled receptor (GPCR) signaling pathways between XY and YY testis.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
YY super-males have rarely been detected in nature and only been artificially created in some fish species including tilapia and yellow catfish (Pelteobagrusfulvidraco), which provides a promising model for testis development and spermatogenesis. In our previous study, significant differences in morphology and miRNA expression were detected between XY and YY testis of yellow catfish. Here, solexa sequencing technology was further performed to compare mRNA expression between XY and YY testis. Compared with unigenes expressed in XY testis, 1146 and 1235 unigenes have significantly higher and lower expression in YY testis, respectively. 605 differentially expressed unigenes were annotated to 1604 GO terms with 319 and 286 genes having relative higher expression in XY and YY testis. KEGG analysis suggested different levels of PI3K-AKT and G protein-coupled receptor (GPCR) signaling pathways between XY and YY testis. Down-regulation of miR-141/429 in YY testis was speculated to promote testis development and maturation, and several factors in PI3K-AKT and GPCR signaling pathways were found as predicted targets of miR-141/429, several of which were confirmed by dual-luciferase reporter assays. Our study provides a comparative transcriptome analysis between XY and YY testis, and reveals interactions between miRNAs and their target genes that are possibly involved in regulating testis development and spermatogenesis.

No MeSH data available.


Related in: MedlinePlus