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Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica.

Meisel RP, Scott JG, Clark AG - Genome Biol Evol (2015)

Bottom Line: To test whether the III(M) chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine whether isogenic males that differ only in the presence of the Y(M) or III(M) chromosome have different gene expression profiles.We find that more genes are differentially expressed between Y(M) and III(M) males in testis than head, and that genes with male-biased expression are most likely to be differentially expressed between Y(M) and III(M) males.We additionally find that III(M) males have a "masculinized" gene expression profile, suggesting that the III(M) chromosome has accumulated an excess of male-beneficial alleles because of its male-limited transmission.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biochemistry, University of Houston rpmeisel@uh.edu.

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Sex-biased expression of genes differentially expressed between YM and IIIM males. Bar graphs indicate the percentage of genes with male-biased (blue), female-biased (pink), or unbiased (gray) expression in either (A) head or (B) gonad that are differentially expressed between YM and IIIM males in either (A) head or (B) testis. P values are for FET between groups.
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evv128-F3: Sex-biased expression of genes differentially expressed between YM and IIIM males. Bar graphs indicate the percentage of genes with male-biased (blue), female-biased (pink), or unbiased (gray) expression in either (A) head or (B) gonad that are differentially expressed between YM and IIIM males in either (A) head or (B) testis. P values are for FET between groups.

Mentions: Genes whose expression is significantly higher in males than females are said to have “male-biased” expression, and genes that are upregulated in females have “female-biased” expression (Ellegren and Parsch 2007). We found that genes with male-biased expression in head are more likely to be differentially expressed between YM and IIIM male heads than genes with either female-biased or unbiased expression (fig. 3A). Similarly, genes that are upregulated in testis relative to ovary (testis-biased) are more likely to be differentially expressed between YM and IIIM testes than genes with “ovary-biased” or unbiased expression in gonad (fig. 3B). We repeated this analysis using two replicates of each sample, and we consistently observe that genes with male-biased expression in head or gonad are more likely to be differentially expressed between YM and IIIM males (supplementary figs. S4 and S5, Supplementary Material online).Fig. 3.—


Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica.

Meisel RP, Scott JG, Clark AG - Genome Biol Evol (2015)

Sex-biased expression of genes differentially expressed between YM and IIIM males. Bar graphs indicate the percentage of genes with male-biased (blue), female-biased (pink), or unbiased (gray) expression in either (A) head or (B) gonad that are differentially expressed between YM and IIIM males in either (A) head or (B) testis. P values are for FET between groups.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4524491&req=5

evv128-F3: Sex-biased expression of genes differentially expressed between YM and IIIM males. Bar graphs indicate the percentage of genes with male-biased (blue), female-biased (pink), or unbiased (gray) expression in either (A) head or (B) gonad that are differentially expressed between YM and IIIM males in either (A) head or (B) testis. P values are for FET between groups.
Mentions: Genes whose expression is significantly higher in males than females are said to have “male-biased” expression, and genes that are upregulated in females have “female-biased” expression (Ellegren and Parsch 2007). We found that genes with male-biased expression in head are more likely to be differentially expressed between YM and IIIM male heads than genes with either female-biased or unbiased expression (fig. 3A). Similarly, genes that are upregulated in testis relative to ovary (testis-biased) are more likely to be differentially expressed between YM and IIIM testes than genes with “ovary-biased” or unbiased expression in gonad (fig. 3B). We repeated this analysis using two replicates of each sample, and we consistently observe that genes with male-biased expression in head or gonad are more likely to be differentially expressed between YM and IIIM males (supplementary figs. S4 and S5, Supplementary Material online).Fig. 3.—

Bottom Line: To test whether the III(M) chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine whether isogenic males that differ only in the presence of the Y(M) or III(M) chromosome have different gene expression profiles.We find that more genes are differentially expressed between Y(M) and III(M) males in testis than head, and that genes with male-biased expression are most likely to be differentially expressed between Y(M) and III(M) males.We additionally find that III(M) males have a "masculinized" gene expression profile, suggesting that the III(M) chromosome has accumulated an excess of male-beneficial alleles because of its male-limited transmission.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biochemistry, University of Houston rpmeisel@uh.edu.

Show MeSH
Related in: MedlinePlus