Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica.
Bottom Line: An M factor on the third chromosome (III(M)) has reached high frequencies in multiple populations across the world, but the evolutionary forces responsible for the invasion of III(M) are not resolved.To test whether the III(M) chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine whether isogenic males that differ only in the presence of the Y(M) or III(M) chromosome have different gene expression profiles.We additionally find that III(M) males have a "masculinized" gene expression profile, suggesting that the III(M) chromosome has accumulated an excess of male-beneficial alleles because of its male-limited transmission.
Affiliation: Department of Biology and Biochemistry, University of Houston firstname.lastname@example.org.Show MeSH
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Mentions: Genes whose expression is significantly higher in males than females are said to have “male-biased” expression, and genes that are upregulated in females have “female-biased” expression (Ellegren and Parsch 2007). We found that genes with male-biased expression in head are more likely to be differentially expressed between YM and IIIM male heads than genes with either female-biased or unbiased expression (fig. 3A). Similarly, genes that are upregulated in testis relative to ovary (testis-biased) are more likely to be differentially expressed between YM and IIIM testes than genes with “ovary-biased” or unbiased expression in gonad (fig. 3B). We repeated this analysis using two replicates of each sample, and we consistently observe that genes with male-biased expression in head or gonad are more likely to be differentially expressed between YM and IIIM males (supplementary figs. S4 and S5, Supplementary Material online).Fig. 3.—
Affiliation: Department of Biology and Biochemistry, University of Houston email@example.com.