Limits...
Variation in the X:Autosome Distribution of Male-Biased Genes among Drosophila melanogaster Tissues and Its Relationship with Dosage Compensation.

Huylmans AK, Parsch J - Genome Biol Evol (2015)

Bottom Line: Previous studies of Drosophila melanogaster found a general paucity of male-biased genes on the X chromosome, although this is mainly limited to comparisons of whole flies or body segments containing the reproductive organs.The brain and head also differ from other tissues in that their male-biased genes are significantly closer to binding sites of the dosage compensation complex.We propose that the interplay of dosage compensation and sex-specific regulation can explain the observed differences between tissues and reconcile disparate results reported in previous studies.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology, Ludwig Maximilian University of Munich, Planegg, Germany.

Show MeSH
Expression level of DCC components in RNA-seq data sets. Expression level was measured in terms of RPKM. The data are from data sets 1, 2, 3, 4, 7, 8, 12, and 13 (table 1). Error bars indicate the standard error of the mean. MLE and MSL-2 are important for initial recognition of DCC binding sites and their colocalization defines the HAS, whereas MSL-1 and MOF do not colocalize with the other DCC components and are not specific to the X chromosome (Straub et al. 2013).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4524484&req=5

evv117-F5: Expression level of DCC components in RNA-seq data sets. Expression level was measured in terms of RPKM. The data are from data sets 1, 2, 3, 4, 7, 8, 12, and 13 (table 1). Error bars indicate the standard error of the mean. MLE and MSL-2 are important for initial recognition of DCC binding sites and their colocalization defines the HAS, whereas MSL-1 and MOF do not colocalize with the other DCC components and are not specific to the X chromosome (Straub et al. 2013).

Mentions: To investigate possible differences in the level of dosage compensation among tissues, we compared the expression of the five DCC components among all tissues for which RNA-seq data were available. For three DCC components (MLE, MSL-2, and MSL-3), we observe the highest expression in the brain and head (fig. 5). This is especially true for MLE and MSL-2, which colocalize to the HAS at which dosage compensation is initiated (Straub et al. 2013). For both MLE and MSL-2, the expression level in brain and head is approximately 2-fold higher than that in other tissues (fig. 5). This suggests that gene expression in the brain and head may be particularly sensitive to DCC-induced upregulation.Fig. 5.—


Variation in the X:Autosome Distribution of Male-Biased Genes among Drosophila melanogaster Tissues and Its Relationship with Dosage Compensation.

Huylmans AK, Parsch J - Genome Biol Evol (2015)

Expression level of DCC components in RNA-seq data sets. Expression level was measured in terms of RPKM. The data are from data sets 1, 2, 3, 4, 7, 8, 12, and 13 (table 1). Error bars indicate the standard error of the mean. MLE and MSL-2 are important for initial recognition of DCC binding sites and their colocalization defines the HAS, whereas MSL-1 and MOF do not colocalize with the other DCC components and are not specific to the X chromosome (Straub et al. 2013).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524484&req=5

evv117-F5: Expression level of DCC components in RNA-seq data sets. Expression level was measured in terms of RPKM. The data are from data sets 1, 2, 3, 4, 7, 8, 12, and 13 (table 1). Error bars indicate the standard error of the mean. MLE and MSL-2 are important for initial recognition of DCC binding sites and their colocalization defines the HAS, whereas MSL-1 and MOF do not colocalize with the other DCC components and are not specific to the X chromosome (Straub et al. 2013).
Mentions: To investigate possible differences in the level of dosage compensation among tissues, we compared the expression of the five DCC components among all tissues for which RNA-seq data were available. For three DCC components (MLE, MSL-2, and MSL-3), we observe the highest expression in the brain and head (fig. 5). This is especially true for MLE and MSL-2, which colocalize to the HAS at which dosage compensation is initiated (Straub et al. 2013). For both MLE and MSL-2, the expression level in brain and head is approximately 2-fold higher than that in other tissues (fig. 5). This suggests that gene expression in the brain and head may be particularly sensitive to DCC-induced upregulation.Fig. 5.—

Bottom Line: Previous studies of Drosophila melanogaster found a general paucity of male-biased genes on the X chromosome, although this is mainly limited to comparisons of whole flies or body segments containing the reproductive organs.The brain and head also differ from other tissues in that their male-biased genes are significantly closer to binding sites of the dosage compensation complex.We propose that the interplay of dosage compensation and sex-specific regulation can explain the observed differences between tissues and reconcile disparate results reported in previous studies.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology, Ludwig Maximilian University of Munich, Planegg, Germany.

Show MeSH